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Literature summary for 2.7.1.185 extracted from
- Attempts to develop an enzyme converting DHIV to KIV (2019), Protein Eng. Des. Sel., 32, 261-270 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of the PtM3K gene under a J23105 promoter | Picrophilus torridus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Picrophilus torridus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-mevalonate | Picrophilus torridus | - |
ADP + (R)-3-phosphomevalonate | - |
? | |
| ATP + (R)-mevalonate | Picrophilus torridus ATCC 700027 | - |
ADP + (R)-3-phosphomevalonate | - |
? | |
| ATP + (R)-mevalonate | Picrophilus torridus NBRC 100828 | - |
ADP + (R)-3-phosphomevalonate | - |
? | |
| ATP + (R)-mevalonate | Picrophilus torridus DSM 9790 | - |
ADP + (R)-3-phosphomevalonate | - |
? | |
| ATP + (R)-mevalonate | Picrophilus torridus JCM 10055 | - |
ADP + (R)-3-phosphomevalonate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Picrophilus torridus | Q6KZB1 | - |
- |
| Picrophilus torridus ATCC 700027 | Q6KZB1 | - |
- |
| Picrophilus torridus DSM 9790 | Q6KZB1 | - |
- |
| Picrophilus torridus JCM 10055 | Q6KZB1 | - |
- |
| Picrophilus torridus NBRC 100828 | Q6KZB1 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-2,3-dihydroxyisovalerate | the Picrophilus torridus mevalonate-3-kinase (M3K) exhibits adenosine triphosphate (ATP) hydrolysis activity when mixed with (R)-2,3-dihydroxyisovalerate (DHIV), indicating phosphorylation activity towards DHIV | Picrophilus torridus | ADP + (R)-2-hydroxy-3-phosphoisovalerate | - |
? | |
| ATP + (R)-2,3-dihydroxyisovalerate | the Picrophilus torridus mevalonate-3-kinase (M3K) exhibits adenosine triphosphate (ATP) hydrolysis activity when mixed with (R)-2,3-dihydroxyisovalerate (DHIV), indicating phosphorylation activity towards DHIV | Picrophilus torridus ATCC 700027 | ADP + (R)-2-hydroxy-3-phosphoisovalerate | - |
? | |
| ATP + (R)-2,3-dihydroxyisovalerate | the Picrophilus torridus mevalonate-3-kinase (M3K) exhibits adenosine triphosphate (ATP) hydrolysis activity when mixed with (R)-2,3-dihydroxyisovalerate (DHIV), indicating phosphorylation activity towards DHIV | Picrophilus torridus NBRC 100828 | ADP + (R)-2-hydroxy-3-phosphoisovalerate | - |
? | |
| ATP + (R)-2,3-dihydroxyisovalerate | the Picrophilus torridus mevalonate-3-kinase (M3K) exhibits adenosine triphosphate (ATP) hydrolysis activity when mixed with (R)-2,3-dihydroxyisovalerate (DHIV), indicating phosphorylation activity towards DHIV | Picrophilus torridus DSM 9790 | ADP + (R)-2-hydroxy-3-phosphoisovalerate | - |
? | |
| ATP + (R)-2,3-dihydroxyisovalerate | the Picrophilus torridus mevalonate-3-kinase (M3K) exhibits adenosine triphosphate (ATP) hydrolysis activity when mixed with (R)-2,3-dihydroxyisovalerate (DHIV), indicating phosphorylation activity towards DHIV | Picrophilus torridus JCM 10055 | ADP + (R)-2-hydroxy-3-phosphoisovalerate | - |
? | |
| ATP + (R)-mevalonate | - |
Picrophilus torridus | ADP + (R)-3-phosphomevalonate | - |
? | |
| ATP + (R)-mevalonate | - |
Picrophilus torridus ATCC 700027 | ADP + (R)-3-phosphomevalonate | - |
? | |
| ATP + (R)-mevalonate | - |
Picrophilus torridus NBRC 100828 | ADP + (R)-3-phosphomevalonate | - |
? | |
| ATP + (R)-mevalonate | - |
Picrophilus torridus DSM 9790 | ADP + (R)-3-phosphomevalonate | - |
? | |
| ATP + (R)-mevalonate | - |
Picrophilus torridus JCM 10055 | ADP + (R)-3-phosphomevalonate | - |
? | |
| additional information | a first reaction strategy is the activaion of (R)-2,3-dihydroxyisovalerate (DHIV) to 3-phospho-DHIV using M3K followed by dehydration by PpManR, StPutD or other sugar acid dehydratases. A second strategy is to use M3K and/or mevalonate decarboxylase (MVD) to convert DHIV to isobutyraldehyde, which can be an alternative pathway for isobutanol biosynthesis | Picrophilus torridus | ? | - |
- |
|
| additional information | a first reaction strategy is the activaion of (R)-2,3-dihydroxyisovalerate (DHIV) to 3-phospho-DHIV using M3K followed by dehydration by PpManR, StPutD or other sugar acid dehydratases. A second strategy is to use M3K and/or mevalonate decarboxylase (MVD) to convert DHIV to isobutyraldehyde, which can be an alternative pathway for isobutanol biosynthesis | Picrophilus torridus ATCC 700027 | ? | - |
- |
|
| additional information | a first reaction strategy is the activaion of (R)-2,3-dihydroxyisovalerate (DHIV) to 3-phospho-DHIV using M3K followed by dehydration by PpManR, StPutD or other sugar acid dehydratases. A second strategy is to use M3K and/or mevalonate decarboxylase (MVD) to convert DHIV to isobutyraldehyde, which can be an alternative pathway for isobutanol biosynthesis | Picrophilus torridus NBRC 100828 | ? | - |
- |
|
| additional information | a first reaction strategy is the activaion of (R)-2,3-dihydroxyisovalerate (DHIV) to 3-phospho-DHIV using M3K followed by dehydration by PpManR, StPutD or other sugar acid dehydratases. A second strategy is to use M3K and/or mevalonate decarboxylase (MVD) to convert DHIV to isobutyraldehyde, which can be an alternative pathway for isobutanol biosynthesis | Picrophilus torridus DSM 9790 | ? | - |
- |
|
| additional information | a first reaction strategy is the activaion of (R)-2,3-dihydroxyisovalerate (DHIV) to 3-phospho-DHIV using M3K followed by dehydration by PpManR, StPutD or other sugar acid dehydratases. A second strategy is to use M3K and/or mevalonate decarboxylase (MVD) to convert DHIV to isobutyraldehyde, which can be an alternative pathway for isobutanol biosynthesis | Picrophilus torridus JCM 10055 | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| M3K | - |
Picrophilus torridus |
| mevalonate-3-kinase | - |
Picrophilus torridus |
| PtM3K | - |
Picrophilus torridus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Picrophilus torridus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Picrophilus torridus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is specialized as a mevalonate 3-kinase catalyzing the first step of the mevalonate decarboxylation (MVD) reaction | Picrophilus torridus |
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