Literature summary for 2.7.1.178 extracted from

Lamble, H.J.; Theodossis, A.; Milburn, C.C.; Taylor, G.L.;, Bull, S.D.; Hough, D.W.; Danson, M.J.
Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus (2005), FEBS Lett., 579, 6865-6869.

Search for more literature for 2.7.1.178

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.8	-	ATP	pH 7.5, 60°C	Saccharolobus solfataricus
3.6	-	2-dehydro-3-deoxy-D-gluconate	pH 7.5, 60°C	Saccharolobus solfataricus
8.1	-	2-dehydro-3-deoxy-D-galactonate	pH 7.5, 60°C	Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34875	-	x * 34875, calculated from sequence	Saccharolobus solfataricus
37513	-	x * 37513, mass spectrometric analysis	Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 2-dehydro-3-deoxy-D-galactonate	Saccharolobus solfataricus	-	ADP + 2-dehydro-3-deoxy-D-galactonate 6-phosphate	-	?
ATP + 2-dehydro-3-deoxy-D-gluconate	Saccharolobus solfataricus	-	ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q97U29	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + 2-dehydro-3-deoxy-D-galactonate = ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate	(2)	Saccharolobus solfataricus	a
ATP + 2-dehydro-3-deoxy-D-gluconate = ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate	(1)	Saccharolobus solfataricus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 2-dehydro-3-deoxy-D-galactonate	-	Saccharolobus solfataricus	ADP + 2-dehydro-3-deoxy-D-galactonate 6-phosphate	-	?
ATP + 2-dehydro-3-deoxy-D-galactonate	2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate are phosphorylated with similar catalytic efficiency	Saccharolobus solfataricus	ADP + 2-dehydro-3-deoxy-D-galactonate 6-phosphate	-	?
ATP + 2-dehydro-3-deoxy-D-gluconate	-	Saccharolobus solfataricus	ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate	-	?
ATP + 2-dehydro-3-deoxy-D-gluconate	2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate are phosphorylated with similar catalytic efficiency	Saccharolobus solfataricus	ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate	-	?

Subunits

Subunits	Comment	Organism
?	x * 34875, calculated from sequence	Saccharolobus solfataricus
?	x * 37513, mass spectrometric analysis	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
KDG kinase	-	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	assay at	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.8	-	ATP	pH 7.5, 60°C	Saccharolobus solfataricus
5	-	2-dehydro-3-deoxy-D-gluconate	pH 7.5, 60°C	Saccharolobus solfataricus
5.4	-	2-dehydro-3-deoxy-D-galactonate	pH 7.5, 60°C	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.7	-	2-dehydro-3-deoxy-D-galactonate	pH 7.5, 60°C	Saccharolobus solfataricus
1.4	-	ATP	pH 7.5, 60°C	Saccharolobus solfataricus
1.4	-	2-dehydro-3-deoxy-D-gluconate	pH 7.5, 60°C	Saccharolobus solfataricus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)