Literature summary for 2.7.1.178 extracted from

Potter, J.A.; Kerou, M.; Lamble, H.J.; Bull, S.D.; Hough, D.W.; Danson, M.J.; Taylor, G.L.
The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase (2008), Acta Crystallogr. Sect. D, 64, 1283-1287.

Search for more literature for 2.7.1.178

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapour diffusion method, crystallized in 0.1 M sodium acetate pH 4.1 and 1.4 M NaCl. The crystal structure of apoenzyme is solved by molecular replacement to a resolution of 2.0 A and a ternary complex with 2-dehydro-3-deoxygluconate and an ATP analogue is resolved at 2.1 A. The complex suggests that the structural basis for the enzymes ability to phosphorylate 2-oxo-3-deoxygluconate and 2-dehydro-3-deoxygalactonate is derived from a subtle repositioning of residues that are conserved in homologous nonpromiscuous kinases	Saccharolobus solfataricus

Crystallization (Comment)

Organism

sitting drop vapour diffusion method, crystallized in 0.1 M sodium acetate pH 4.1 and 1.4 M NaCl. The crystal structure of apoenzyme is solved by molecular replacement to a resolution of 2.0 A and a ternary complex with 2-dehydro-3-deoxygluconate and an ATP analogue is resolved at 2.1 A. The complex suggests that the structural basis for the enzymes ability to phosphorylate 2-oxo-3-deoxygluconate and 2-dehydro-3-deoxygalactonate is derived from a subtle repositioning of residues that are conserved in homologous nonpromiscuous kinases

Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q97U29	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)