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Literature summary for 2.7.1.177 extracted from
- The PduX enzyme of Salmonella enterica is an L-threonine kinase used for coenzyme B12 synthesis (2008), J. Biol. Chem., 283, 11322-11329.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0547 | - |
ATP | Michaelis-Menten kinetics, pH 7.4, temperature not specified in the publication | Salmonella enterica |  |
| 0.069 | - |
ATP | calculated from double-reciprocal plots, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.127 | - |
L-threonine | calculated from double-reciprocal plots, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.146 | - |
L-threonine | Michaelis-Menten kinetics, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32787 | - |
x * 32787, calculated from sequence, x * 35000, SDS-PAGE | Salmonella enterica |
| 35000 | - |
x * 32787, calculated from sequence, x * 35000, SDS-PAGE | Salmonella enterica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-threonine | Salmonella enterica | - |
ADP + O-phospho-L-threonine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica | Q9XDM4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Salmonella enterica |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-threonine = ADP + O-phospho-L-threonine | steady-state ordered BiBI mechanism | Salmonella enterica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-threonine | - |
Salmonella enterica | ADP + O-phospho-L-threonine | - |
? | |
| ATP + L-threonine | - |
Salmonella enterica | ADP + O-phospho-L-threonine | product indentified by 31P NMR spectroscopy | ? | |
| additional information | the activity with CTP, GTP, or UTP is 6, 11, 3% of the activity with ATP, respectively | Salmonella enterica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32787, calculated from sequence, x * 35000, SDS-PAGE | Salmonella enterica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PduX | - |
Salmonella enterica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | involved in the biosynthesis of adenosylcobalamin | Salmonella enterica |
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