Literature summary for 2.7.1.174 extracted from

Qiu, Y.; Fakas, S.; Han, G.S.; Barbosa, A.D.; Siniossoglou, S.; Carman, G.M.
Transcription factor Reb1p regulates DGK1-encoded diacylglycerol kinase and lipid metabolism in Saccharomyces cerevisiae (2013), J. Biol. Chem., 288, 29124-29133.

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Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	associated with	Saccharomyces cerevisiae	5783	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	required	Saccharomyces cerevisiae
Mg2+	required	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
CTP + 1,2-diacyl-sn-glycerol	Saccharomyces cerevisiae	-	CDP + 1,2-diacyl-sn-glycerol 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	Q12382	gene DGK1	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CTP + 1,2-diacyl-sn-glycerol	-	Saccharomyces cerevisiae	CDP + 1,2-diacyl-sn-glycerol 3-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
CTP-dependent diacylglycerol kinase	-	Saccharomyces cerevisiae
DAG kinase	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
CTP	-	Saccharomyces cerevisiae

Expression

Organism	Comment	Expression
Saccharomyces cerevisiae	expression of diacylglycerol kinase is induced by the Reb1p transcription factor, Reb1p directly interacts with the Reb1p-binding sequence in the DGK1 promoter	up

General Information

General Information	Comment	Organism
malfunction	a Reb1p-binding site mutation abolishes the DGK1-mediated nuclear/endoplasmic reticulum membrane expansion	Saccharomyces cerevisiae
physiological function	the enzyme catalyzes the CTP-dependent phosphorylation of diacylglycerol to form phosphatidate, and in conjunction with PAH1-encoded phosphatidate phosphatase, it controls the levels of phosphatidate and diacylglycerol for phospholipid synthesis, membrane growth, and lipid droplet formation. A functional level of diacylglycerol kinase is regulated by the Reb1p transcription factor. Reb1p specifically binds the consensus recognition sequence (CGGGTAA, -166 to -160) in the DGK1 promoter. Diacylglycerol kinase is regulated at the level of transcription. The expression of diacylglycerol kinase is induced by the Reb1p transcription factor, and the resulting activity increase is essential for the enzyme function in phospholipid synthesis. The DGK1-encoded DAG kinase activity causes the accumulation of phosphatidic acid at the nuclear/endoplasmicreticulum membrane when the phospholipid is not hydrolyzed by PAH1-encoded phosphatidic acid phosphatase	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)