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Literature summary for 2.7.1.174 extracted from

  • Han, G.S.; OHara, L.; Carman, G.M.; Siniossoglou, S.
    An unconventional diacylglycerol kinase that regulates phospholipid synthesis and nuclear membrane growth (2008), J. Biol. Chem., 283, 20433-20442.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene DGK1 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information overexpression of DGK1 causes the appearance of phosphatidate-enriched membranes around the nucleus and leads to its expansion, without proliferating the cortical endoplasmic reticulum membrane. Deletion of DGK1 returns the phosphatidate and phosphatidylethanolamine levels of the pah1DELTA cells to normal Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane integral membrane protein Saccharomyces cerevisiae 5789
-
nuclear membrane integral membrane protein Saccharomyces cerevisiae 31965
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + 1,2-diacyl-sn-glycerol Saccharomyces cerevisiae
-
CDP + 1,2-diacyl-sn-glycerol 3-phosphate Dgk1p-derived phosphatidate accumulates on the nuclear membrane ?
CTP + 1,2-diacyl-sn-glycerol Saccharomyces cerevisiae BY4742
-
CDP + 1,2-diacyl-sn-glycerol 3-phosphate Dgk1p-derived phosphatidate accumulates on the nuclear membrane ?
additional information Saccharomyces cerevisiae Dgk1p does not function as a CDP-DAG synthase in vivo ?
-
?
additional information Saccharomyces cerevisiae BY4742 Dgk1p does not function as a CDP-DAG synthase in vivo ?
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q12382 gene DGK1
-
Saccharomyces cerevisiae BY4742 Q12382 gene DGK1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 1,2-diacyl-sn-glycerol
-
Saccharomyces cerevisiae CDP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
CTP + 1,2-diacyl-sn-glycerol
-
Saccharomyces cerevisiae CDP + 1,2-diacyl-sn-glycerol 3-phosphate Dgk1p-derived phosphatidate accumulates on the nuclear membrane ?
CTP + 1,2-diacyl-sn-glycerol
-
Saccharomyces cerevisiae BY4742 CDP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?
CTP + 1,2-diacyl-sn-glycerol
-
Saccharomyces cerevisiae BY4742 CDP + 1,2-diacyl-sn-glycerol 3-phosphate Dgk1p-derived phosphatidate accumulates on the nuclear membrane ?
additional information Dgk1p does not function as a CDP-DAG synthase in vivo Saccharomyces cerevisiae ?
-
?
additional information Dgk1p does not function as a CDP-DAG synthase in vivo Saccharomyces cerevisiae BY4742 ?
-
?

Synonyms

Synonyms Comment Organism
CTP-dependent DAG kinase
-
Saccharomyces cerevisiae
DGK1
-
Saccharomyces cerevisiae
Dgk1p
-
Saccharomyces cerevisiae
More formerly HSD1 protein Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
CTP dependent on Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction mutations decrease phosphatidate levels decrease nuclear membrane growth in pah1DELTA cells, the inactivation of Pah1p leads to nuclear growth. A dgk1DLETA strain expressing a mutant DGK1 allele in which residue 177 is changed to alanine does not display any CTP-dependent DAG kinase activity Saccharomyces cerevisiae
metabolism phosphatidate metabolism is a critical factor determining nuclear structure by regulating nuclear membrane biogenesis, DGK1 rescues the lethality of dephosphorylated PAH1 Saccharomyces cerevisiae
additional information role of phospholipid metabolism in nuclear/ER membrane structure Saccharomyces cerevisiae
physiological function Dgk1p is a unique diacylglycerol kinase that uses CTP, instead of ATP, to generate phosphatidate. The unconventional diacylglycerol kinase regulates phospholipid synthesis and nuclear membrane growth, detailed overview. DGK1 counteracts the activity of PAH1 at the nuclear envelope by controlling phosphatidate levels. Phosphatidate metabolism is a critical factor determining nuclear structure by regulating nuclear membrane biogenesis. DGK1 rescues the lethality of dephosphorylated PAH1 Saccharomyces cerevisiae