Cloned (Comment) | Organism |
---|---|
gene DGK1 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of DGK1 causes the appearance of phosphatidate-enriched membranes around the nucleus and leads to its expansion, without proliferating the cortical endoplasmic reticulum membrane. Deletion of DGK1 returns the phosphatidate and phosphatidylethanolamine levels of the pah1DELTA cells to normal | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | integral membrane protein | Saccharomyces cerevisiae | 5789 | - |
nuclear membrane | integral membrane protein | Saccharomyces cerevisiae | 31965 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 1,2-diacyl-sn-glycerol | Saccharomyces cerevisiae | - |
CDP + 1,2-diacyl-sn-glycerol 3-phosphate | Dgk1p-derived phosphatidate accumulates on the nuclear membrane | ? | |
CTP + 1,2-diacyl-sn-glycerol | Saccharomyces cerevisiae BY4742 | - |
CDP + 1,2-diacyl-sn-glycerol 3-phosphate | Dgk1p-derived phosphatidate accumulates on the nuclear membrane | ? | |
additional information | Saccharomyces cerevisiae | Dgk1p does not function as a CDP-DAG synthase in vivo | ? | - |
? | |
additional information | Saccharomyces cerevisiae BY4742 | Dgk1p does not function as a CDP-DAG synthase in vivo | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q12382 | gene DGK1 | - |
Saccharomyces cerevisiae BY4742 | Q12382 | gene DGK1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 1,2-diacyl-sn-glycerol | - |
Saccharomyces cerevisiae | CDP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
CTP + 1,2-diacyl-sn-glycerol | - |
Saccharomyces cerevisiae | CDP + 1,2-diacyl-sn-glycerol 3-phosphate | Dgk1p-derived phosphatidate accumulates on the nuclear membrane | ? | |
CTP + 1,2-diacyl-sn-glycerol | - |
Saccharomyces cerevisiae BY4742 | CDP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
CTP + 1,2-diacyl-sn-glycerol | - |
Saccharomyces cerevisiae BY4742 | CDP + 1,2-diacyl-sn-glycerol 3-phosphate | Dgk1p-derived phosphatidate accumulates on the nuclear membrane | ? | |
additional information | Dgk1p does not function as a CDP-DAG synthase in vivo | Saccharomyces cerevisiae | ? | - |
? | |
additional information | Dgk1p does not function as a CDP-DAG synthase in vivo | Saccharomyces cerevisiae BY4742 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CTP-dependent DAG kinase | - |
Saccharomyces cerevisiae |
DGK1 | - |
Saccharomyces cerevisiae |
Dgk1p | - |
Saccharomyces cerevisiae |
More | formerly HSD1 protein | Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CTP | dependent on | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | mutations decrease phosphatidate levels decrease nuclear membrane growth in pah1DELTA cells, the inactivation of Pah1p leads to nuclear growth. A dgk1DLETA strain expressing a mutant DGK1 allele in which residue 177 is changed to alanine does not display any CTP-dependent DAG kinase activity | Saccharomyces cerevisiae |
metabolism | phosphatidate metabolism is a critical factor determining nuclear structure by regulating nuclear membrane biogenesis, DGK1 rescues the lethality of dephosphorylated PAH1 | Saccharomyces cerevisiae |
additional information | role of phospholipid metabolism in nuclear/ER membrane structure | Saccharomyces cerevisiae |
physiological function | Dgk1p is a unique diacylglycerol kinase that uses CTP, instead of ATP, to generate phosphatidate. The unconventional diacylglycerol kinase regulates phospholipid synthesis and nuclear membrane growth, detailed overview. DGK1 counteracts the activity of PAH1 at the nuclear envelope by controlling phosphatidate levels. Phosphatidate metabolism is a critical factor determining nuclear structure by regulating nuclear membrane biogenesis. DGK1 rescues the lethality of dephosphorylated PAH1 | Saccharomyces cerevisiae |