Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged NRK1 in Escherichia coli strain BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
NRK1 in complex with the reaction product nicotinamide mononucleotide, and NRK1 in a ternary complex with ADP and the anticancer drug tiazofurin, sitting-drop vapor-diffusion method, 22°C, mixing of protein solution containing 20 mg/ml protein, 2 mM ligand, 20 mM Tris, pH 7.5-7.8, 200 mM NaCl, 5 mM DTT, with reservoir solution containing 28% w/v PEG 3350, 200 mM NH4Cl, 5 mM DTT, and 5 mM Na2HPO4, X-ray diffraction structure determination and analysis at 1.5 A and 2.7 A resolution, respectively, modeling | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-ribosylnicotinamide | Homo sapiens | - |
ADP + nicotinamide beta-D-ribonucleotide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NWW6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged NRK1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + beta-D-ribosylnicotinate = ADP + nicotinate beta-D-ribonucleotide | catalytic mechanism, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-ribosylnicotinamide | - |
Homo sapiens | ADP + nicotinamide beta-D-ribonucleotide | - |
? | |
ATP + nicotinamide mononucleotide | binding mode of the substrate, overview | Homo sapiens | ADP + nicotinamide mononucleotide phosphate | - |
? | |
ATP + tiazofurin | binding mode of the substrate, overview | Homo sapiens | ADP + ? | - |
? | |
additional information | ADP binding mode, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the structure of NRK1 contains a central, five-stranded parallel beta sheet, with five alpha helices on both faces, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
nicotinamide riboside kinase | - |
Homo sapiens |
Nrk | - |
Homo sapiens |
NRK1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | nicotinamide riboside kinase has an important role in the biosynthesis of NAD+ as well as the activation of tiazofurin and other nicotinamide riboside analogues for anticancer therapy | Homo sapiens |