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Literature summary for 2.7.1.172 extracted from

  • Fortpied, J.; Gemayel, R.; Stroobant, V.; van Schaftingen, E.
    Plant ribulosamine/erythrulosamine 3-kinase, a putative protein-repair enzyme (2005), Biochem. J., 388, 795-802.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.172

Cloned(Commentary)

Cloned (Comment) Organism
produced in Escherichia coli as an N-terminal fusion protein with His6 tag Spinacia oleracea
produced in Escherichia coli as an N-terminal fusion protein with His6 tag Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
1-deoxy-1-morpholino-D-ribulose 0.25 mM, 50% inhibition Spinacia oleracea
additional information neither fructosamines (fructoselysine and deoxymorpholinofructose) nor psicoselysine affect the enzymatic activity at concentrations up to 1 mM. No inhibition is observed with ribulosamines bound to the alpha-amino groups of glycine, valine or leucine, or with D-ribulose or D-erythrulose (all tested at 1 mM) Spinacia oleracea
ribuloselysine 0.1 mM, 50% inhibition Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 [lysozyme]-N6-D-erythrulosyl-L-lysine pH 7.1, 30°C Spinacia oleracea
0.025
-
 N6-D-erythrulosyl-L-lysine pH 7.1, 30°C Arabidopsis thaliana
0.104
-
 [lysozyme]-N6-D-erythrulosyl-L-lysine pH 7.1, 30°C Arabidopsis thaliana
0.119
-
 N6-D-ribulosyl-L-lysine pH 7.1, 30°C Spinacia oleracea
0.135
-
 N6-D-ribulosyl-L-lysine pH 7.1, 30°C Arabidopsis thaliana
0.274
-
 [lysozyme]-N6-D-ribulosyl-L-lysine pH 7.1, 30°C Arabidopsis thaliana
0.279
-
 N6-D-erythrulosyl-L-lysine pH 7.1, 30°C Spinacia oleracea
0.659
-
 [lysozyme]-N6-D-ribulosyl-L-lysine pH 7.1, 30°C Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 gel filtration Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea
-
 Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.174
-
 pH 7.1, 30°C, substrate: N5-D-ribulosyl-L-lysine Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N6-D-erythrulosyl-L-lysine
-
 Spinacia oleracea ADP + N6-(O3-phosphono-D-erythrulosyl)-L-lysine
-
 ?
ATP + N6-D-erythrulosyl-L-lysine
-
 Arabidopsis thaliana ADP + N6-(O3-phosphono-D-erythrulosyl)-L-lysine
-
 ?
ATP + N6-D-ribulosyl-L-lysine
-
 Spinacia oleracea ADP + N6-(O3-phosphono-D-ribulosyl)-L-lysine
-
 ?
ATP + N6-D-ribulosyl-L-lysine
-
 Arabidopsis thaliana ADP + N6-(O3-phosphono-D-ribulosyl)-L-lysine
-
 ?
ATP + [lysozyme]-N6-D-erythrulosyl-L-lysine
-
 Spinacia oleracea ADP + [lysozyme]-N6-(O3-phosphono-D-erythrulosyl)-L-lysine
-
 ?
ATP + [lysozyme]-N6-D-erythrulosyl-L-lysine
-
 Arabidopsis thaliana ADP + [lysozyme]-N6-(O3-phosphono-D-erythrulosyl)-L-lysine
-
 ?
ATP + [lysozyme]-N6-D-ribulosyl-L-lysine
-
 Spinacia oleracea ADP + [lysozyme]-N6-(O3-phosphono-D-ribulosyl)-L-lysine
-
 ?
ATP + [lysozyme]-N6-D-ribulosyl-L-lysine
-
 Arabidopsis thaliana ADP + [lysozyme]-N6-(O3-phosphono-D-ribulosyl)-L-lysine
-
 ?
additional information ribuloseglycine, ribulosevaline and ribuloseleucine are phosphorylated at 5–10% of the rate observed with 1 mM ribuloselysine, whereas no detectable phosphorylation (<1% of the rate observed with ribuloselysine) is observed with fructoselysine, deoxymorpholinofructose, D-ribulose and D-erythrulose. The purified enzyme does not phosphorylate protein-bound fructosamines, psicosamines or ribulosamine 5-phosphates, but does act on protein-bound ribulosamines and erythrulosamines Spinacia oleracea ?
-
 ?
additional information the enzyme does not act on fructosy-L-lysine or lysozyme glycated with glucose or allose Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
ribulosamine/erythrulosamine 3-kinase
-
 Spinacia oleracea
ribulosamine/erythrulosamine 3-kinase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Spinacia oleracea
30
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
 assay at Spinacia oleracea
7.1
-
 assay at Arabidopsis thaliana