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Literature summary for 2.7.1.171 extracted from

  • De Bruyne, S.; Van Dorpe, J.; Himpe, J.; Van Biesen, W.; Delanghe, S.; Speeckaert, M.M.; Delanghe, J.R.
    Detection and characterization of a biochemical signature associated with diabetic nephropathy using near-infrared spectroscopy on tissue sections (2019), J. Clin. Med., 8, 2869 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.171

Cloned(Commentary)

Cloned (Comment) Organism
gene FN3K, constitutive expression, recombinant expression of N-terminally His6-tagged enzyme from the codon-optimized gene in Pichia pastoris under control of the methanol inducible aldehyde oxidase 1 (AOX1) promoter Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [protein]-N6-D-fructosyl-L-lysine Homo sapiens
-
 ADP + [protein]-N6-(3-O-phospho-D-fructosyl)-L-lysine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9H479
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged enzyme from Pichia pastoris by nickel affinity chromatography and gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
erythrocyte
-
 Homo sapiens
-
eye
-
 Homo sapiens
-
kidney
-
 Homo sapiens
-
lens
-
 Homo sapiens
-
additional information the enzyme is more active in tissues with a long half-life (e.g. brain, erythrocytes and lens), but the expression of the genes for FN3K appears to be constitutive and unaffected by environmental signals Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [protein]-N6-D-fructosyl-L-lysine
-
 Homo sapiens ADP + [protein]-N6-(3-O-phospho-D-fructosyl)-L-lysine
-
 ?

Synonyms

Synonyms Comment Organism
FN3K
-
 Homo sapiens
fructosamine-3-kinase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens the expression of the genes for FN3K appears to be constitutive and unaffected by environmental signals additional information

General Information

General Information Comment Organism
physiological function fructosamine-3-kinase (FN3K) is involved in natural cellular repair mechanisms to control non-enzymatic glycation of proteins. It also has potential in the disruption of retinal advanced glycation end products (AGEs), which are important risk factor in pathogenesis in complement, lipid, angiogenic, inflammatory and extracellular matrix pathways in the eye. Increased levels of AGEs have been found in the Bruch's membrane, retinal pigment epithelium (RPE) and drusen of patients with age-related macular degeneration (AMD), a degenerative disorder of the macular region of the retina. Analysis of FN3K treatment of AGE-modified neural porcine, murine, and human retinas. The treatment reduces AGE-related autofluorescence. Murine and human eyes treated intravitreally with FN3K show less drusenoid material and lesions on stained tissue sections. Biochemical changes after FN3K treatment, overview. Near-infrared (NIR) microspectroscopy of the Bruch's membrane and drusen on hematoxylin and eosin stained slides originating from two patients with stage 3 age-related macular degeneration (AMD). Vivo intravitreal FN3K treatment on human eyes strongly reduces size of subretinal drusenoid deposits on optical coherence tomography Homo sapiens