Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | strong inhibitor of AnmK | Escherichia coli | |
additional information | AnmK is not inhibited by N-acetyl-beta-muramate 6-phosphate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
ATP | in 100 mM Tris-HCl (pH 7.5), 20 mM MgCl2, at 37°C | Escherichia coli | |
1 | - |
1,6-anhydro-N-acetyl-beta-muramate | in 100 mM Tris-HCl (pH 7.5), 20 mM MgCl2, at 37°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39500 | - |
calculated from amino acid sequence | Escherichia coli |
40000 | - |
SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli MG1655 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sephacel column chromatography, hydroxylapatite column chromatography, and Mono Q column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,6-anhydro-N-acetyl-beta-muramate + H2O | - |
Escherichia coli | ADP + N-acetyl-beta-muramate 6-phosphate + H+ | - |
? | |
ATP + 1,6-anhydro-N-acetyl-beta-muramate + H2O | - |
Escherichia coli MG1655 | ADP + N-acetyl-beta-muramate 6-phosphate + H+ | - |
? | |
additional information | AnmK does not phosphorylate N-acetyl-muramate | Escherichia coli | ? | - |
? | |
additional information | AnmK does not phosphorylate N-acetyl-muramate | Escherichia coli MG1655 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
anhMurNAc kinase | - |
Escherichia coli |
anhydro-N-acetylmuramic acid kinase | - |
Escherichia coli |
AnmK | - |
Escherichia coli |
YdhH | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
from no activity at pH 4.5 and 5% of the maximum activity at pH 6.0, the activity continues to increase to pH 10.0 | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
ADP | in 100 mM Tris-HCl (pH 7.5), 20 mM MgCl2, at 37°C | Escherichia coli |