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Literature summary for 2.7.1.17 extracted from

  • Wang, R.; Zhang, L.; Wang, D.; Gao, X.; Hong, J.
    Identification of a xylulokinase catalyzing xylulose phosphorylation in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777 (2011), J. Ind. Microbiol. Biotechnol., 38, 1739-1746.
    View publication on PubMed
Search for more literature for 2.7.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Kluyveromyces marxianus

Organism

Organism UniProt Comment Textmining
Kluyveromyces marxianus F6JVF3
-
-
Kluyveromyces marxianus NBRC1777 F6JVF3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sephacryl S200 gel filtration Kluyveromyces marxianus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.5
-
 using D-xylulose as substrate, 50 mM Tris-HCl (pH 7.5), at 25°C Kluyveromyces marxianus
46
-
 using D-fructose as substrate, 50 mM Tris-HCl (pH 7.5), at 25°C Kluyveromyces marxianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose
-
 Kluyveromyces marxianus ADP + D-fructose 5-phosphate
-
 ?
ATP + D-fructose
-
 Kluyveromyces marxianus NBRC1777 ADP + D-fructose 5-phosphate
-
 ?
ATP + D-xylulose
-
 Kluyveromyces marxianus ADP + D-xylulose 5-phosphate
-
 ?
ATP + D-xylulose
-
 Kluyveromyces marxianus NBRC1777 ADP + D-xylulose 5-phosphate
-
 ?
additional information no activity with D-xylose Kluyveromyces marxianus ?
-
 ?
additional information no activity with D-xylose Kluyveromyces marxianus NBRC1777 ?
-
 ?

Synonyms

Synonyms Comment Organism
D-xylulokinase
-
 Kluyveromyces marxianus
XYL3
-
 Kluyveromyces marxianus

General Information

General Information Comment Organism
malfunction disruption of XYL3 results in both loss of xylitol utilization and marked decrease in xylose utilization Kluyveromyces marxianus
metabolism the enzyme is critical for xylose and xylitol utilization Kluyveromyces marxianus