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Literature summary for 2.7.1.17 extracted from

  • Matsushika, A.; Watanabe, S.; Kodaki, T.; Makino, K.; Sawayama, S.
    Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP(+)-dependent xylitol dehydrogenase, and xylulokinase (2008), J. Biosci. Bioeng., 105, 296-299.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain D452-2, DNA from Saccharomyces cerevisiae strain S288C, strain D-XR/ARSdR with naturally occuring XK-activity Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae strain D452-2, DNA from Saccharomyces cerevisiae strain S288C, strain D-XR/ARSdR/XK with upegulated XK-activity, entire coding sequence of XK with PGK promoter and terminator inserted into pAUR101 shuttle vector (Takara Bio, Kyoto) integrating in Saccharomyces cerevisiae strain D452-2 Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae strain D452-2, DNA from Saccharomyces cerevisiae strain S288C, strain D-XR/XDH with naturally occuring enzyme-activity Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae strain D452-2, DNA from Saccharomyces cerevisiae strain S288C, strain D-XR/XDH/XK with upegulated XK-activity, entire coding sequence of XK with PGK promoter and terminator inserted into pAUR101 shuttle vector integrating in Saccharomyces cerevisiae strain D452-2 Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae D452-2 D-XR/ARSdR
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-
-
Saccharomyces cerevisiae D452-2 D-XR/ARSdR/XK
-
-
-
Saccharomyces cerevisiae D452-2 D-XR/XDH
-
-
-
Saccharomyces cerevisiae D452-2 D-XR/XDH/XK
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
0.034 +/-0.002 U/mg, more than 2fold higher values than D-XR/XDH and D-XR/ARSdR, XK reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute. Saccharomyces cerevisiae
additional information
-
strain D-XR/ARSdR/XK, 0.030 +/-0.002 U/mg, more than two-fold higher values than D-XR/XDH and D-XR/ARSdR, reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute. Saccharomyces cerevisiae
additional information
-
strain D452-2 D-XR/ARSdR, 0.013 +/-0.001 U/mg, reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute. Saccharomyces cerevisiae
additional information
-
strain D452-2 D-XR/XDH, 0.014 +/-0.002 U/mg, reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute. Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-xylulose
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Saccharomyces cerevisiae ADP + D-xylulose 5-phosphate
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r
ATP + D-xylulose
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Saccharomyces cerevisiae D452-2 D-XR/XDH/XK ADP + D-xylulose 5-phosphate
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r
ATP + D-xylulose
-
Saccharomyces cerevisiae D452-2 D-XR/ARSdR/XK ADP + D-xylulose 5-phosphate
-
r
ATP + D-xylulose
-
Saccharomyces cerevisiae D452-2 D-XR/XDH ADP + D-xylulose 5-phosphate
-
r
ATP + D-xylulose
-
Saccharomyces cerevisiae D452-2 D-XR/ARSdR ADP + D-xylulose 5-phosphate
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r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Saccharomyces cerevisiae