Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain D452-2, DNA from Saccharomyces cerevisiae strain S288C, strain D-XR/ARSdR with naturally occuring XK-activity | Saccharomyces cerevisiae |
expressed in Saccharomyces cerevisiae strain D452-2, DNA from Saccharomyces cerevisiae strain S288C, strain D-XR/ARSdR/XK with upegulated XK-activity, entire coding sequence of XK with PGK promoter and terminator inserted into pAUR101 shuttle vector (Takara Bio, Kyoto) integrating in Saccharomyces cerevisiae strain D452-2 | Saccharomyces cerevisiae |
expressed in Saccharomyces cerevisiae strain D452-2, DNA from Saccharomyces cerevisiae strain S288C, strain D-XR/XDH with naturally occuring enzyme-activity | Saccharomyces cerevisiae |
expressed in Saccharomyces cerevisiae strain D452-2, DNA from Saccharomyces cerevisiae strain S288C, strain D-XR/XDH/XK with upegulated XK-activity, entire coding sequence of XK with PGK promoter and terminator inserted into pAUR101 shuttle vector integrating in Saccharomyces cerevisiae strain D452-2 | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae D452-2 D-XR/ARSdR | - |
- |
- |
Saccharomyces cerevisiae D452-2 D-XR/ARSdR/XK | - |
- |
- |
Saccharomyces cerevisiae D452-2 D-XR/XDH | - |
- |
- |
Saccharomyces cerevisiae D452-2 D-XR/XDH/XK | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
0.034 +/-0.002 U/mg, more than 2fold higher values than D-XR/XDH and D-XR/ARSdR, XK reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute. | Saccharomyces cerevisiae |
additional information | - |
strain D-XR/ARSdR/XK, 0.030 +/-0.002 U/mg, more than two-fold higher values than D-XR/XDH and D-XR/ARSdR, reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute. | Saccharomyces cerevisiae |
additional information | - |
strain D452-2 D-XR/ARSdR, 0.013 +/-0.001 U/mg, reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute. | Saccharomyces cerevisiae |
additional information | - |
strain D452-2 D-XR/XDH, 0.014 +/-0.002 U/mg, reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute. | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-xylulose | - |
Saccharomyces cerevisiae | ADP + D-xylulose 5-phosphate | - |
r | |
ATP + D-xylulose | - |
Saccharomyces cerevisiae D452-2 D-XR/XDH/XK | ADP + D-xylulose 5-phosphate | - |
r | |
ATP + D-xylulose | - |
Saccharomyces cerevisiae D452-2 D-XR/ARSdR/XK | ADP + D-xylulose 5-phosphate | - |
r | |
ATP + D-xylulose | - |
Saccharomyces cerevisiae D452-2 D-XR/XDH | ADP + D-xylulose 5-phosphate | - |
r | |
ATP + D-xylulose | - |
Saccharomyces cerevisiae D452-2 D-XR/ARSdR | ADP + D-xylulose 5-phosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |