Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method using 1.5 M ammonium sulfate, 50 mM sodium citrate, pH 6, 1% (w/v) t-butanol and 25% (v/v) ethylene glycol, at 25°C | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D233A | loss of activity | Escherichia coli |
D6A | 19fold decreased ATPase activity compared to the wild type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluoro-xylulose | 0.18 mM, strong inhibition of the ATPase activity of xylulokinase | Escherichia coli | |
AMPPNP | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
D-xylulose | wild type enzyme | Escherichia coli | |
8.2 | - |
ATP | mutant D233A | Escherichia coli | |
14 | - |
D-ribulose | wild type enzyme | Escherichia coli | |
127 | - |
xylitol | wild type enzyme | Escherichia coli | |
141 | - |
D-arabitol | wild type enzyme | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, dynamic light scattering | Escherichia coli |
110000 | - |
dynamic light scattering | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P09099 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-arabitol | - |
Escherichia coli | ADP + D-arabitol 5-phosphate | - |
? | |
ATP + D-ribulose | - |
Escherichia coli | ADP + D-ribulose 5-phosphate | - |
? | |
ATP + D-xylulose | - |
Escherichia coli | ADP + D-xylulose 5-phosphate | - |
r | |
ATP + xylitol | - |
Escherichia coli | ADP + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, dynamic light scattering | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0038 | - |
ATP | mutant D6A | Escherichia coli | |
0.13 | - |
ATP | mutant D233A | Escherichia coli | |
105 | - |
D-arabitol | wild type enzyme | Escherichia coli | |
235 | - |
D-ribulose | wild type enzyme | Escherichia coli | |
237 | - |
xylitol | wild type enzyme | Escherichia coli | |
255 | - |
D-xylulose | wild type enzyme | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
5-fluoro-xylulose | in the presence of 5 mM ATP | Escherichia coli | |
0.15 | - |
5-fluoro-xylulose | in the presence of 0.167 mM ATP | Escherichia coli | |
0.71 | - |
AMPPNP | in the presence of 4.25 mM xylulose | Escherichia coli | |
1.1 | - |
AMPPNP | in the presence of 0.28 mM xylulose | Escherichia coli | |
1.9 | - |
AMPPNP | in the presence of 0.167 mM ATP | Escherichia coli |