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Literature summary for 2.7.1.17 extracted from

  • Dmytruk, O.V.; Voronovsky, A.Y.; Abbas, C.A.; Dmytruk, K.V.; Ishchuk, O.P.; Sibirny, A.A.
    Overexpression of bacterial xylose isomerase and yeast host xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha (2008), FEMS Yeast Res., 8, 165-173.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Hansenula polymorpha strain CBS4732 double mutant with deleted genes of both XR (xylose reductase) and XDH (NAD-dependent xylitol dehydrogenase), expression driven by the Hansenula polymorpha glyceraldehyde-3-phosphate dehydrogenase promoter Ogataea angusta
expressed in Hansenula polymorpha strain CBS4732s leu2-2 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
strain DH5alpha
-
Escherichia coli DH5-alpha
-
strain DH5alpha
-
Ogataea angusta
-
endogenous XYL3 gene
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
0.07 units/mg, at 37°C, in Tris-HCl buffer (pH 7.5) 100 mM, MgCl2 10 mM, NADH 0.15 mM, sorbitol dehydrogenase 2 units, and xylose 500 mM Escherichia coli
additional information
-
0.144 +/-0.008 U/mg protein, the transformant grown in YNB medium with 4% D-xylose showes 2-fold higher XK activity as compared to that of the wild-type strain, activity in cell extracts is determined spectrophotometrically at 37°C, assay mixture: Tris-HCl (pH 7.8) 50 mM, MgCl2 5 mM, NADH 0.2 mM, phosphoenolpyruvate 1 mM, D-xylulose 8.5 mM, lactate dehydrogenase 10 U, pyruvate kinase 0.05 U, ATP 2 mM Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-xylulose
-
Escherichia coli ADP + D-xylulose 5-phosphate
-
r
ATP + D-xylulose
-
Ogataea angusta ADP + D-xylulose 5-phosphate
-
r
ATP + D-xylulose
-
Escherichia coli DH5-alpha ADP + D-xylulose 5-phosphate
-
r

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 38
-
Ogataea angusta

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli