Cloned (Comment) | Organism |
---|---|
expressed in Hansenula polymorpha strain CBS4732 double mutant with deleted genes of both XR (xylose reductase) and XDH (NAD-dependent xylitol dehydrogenase), expression driven by the Hansenula polymorpha glyceraldehyde-3-phosphate dehydrogenase promoter | Ogataea angusta |
expressed in Hansenula polymorpha strain CBS4732s leu2-2 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strain DH5alpha | - |
Escherichia coli DH5-alpha | - |
strain DH5alpha | - |
Ogataea angusta | - |
endogenous XYL3 gene | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
0.07 units/mg, at 37°C, in Tris-HCl buffer (pH 7.5) 100 mM, MgCl2 10 mM, NADH 0.15 mM, sorbitol dehydrogenase 2 units, and xylose 500 mM | Escherichia coli |
additional information | - |
0.144 +/-0.008 U/mg protein, the transformant grown in YNB medium with 4% D-xylose showes 2-fold higher XK activity as compared to that of the wild-type strain, activity in cell extracts is determined spectrophotometrically at 37°C, assay mixture: Tris-HCl (pH 7.8) 50 mM, MgCl2 5 mM, NADH 0.2 mM, phosphoenolpyruvate 1 mM, D-xylulose 8.5 mM, lactate dehydrogenase 10 U, pyruvate kinase 0.05 U, ATP 2 mM | Ogataea angusta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-xylulose | - |
Escherichia coli | ADP + D-xylulose 5-phosphate | - |
r | |
ATP + D-xylulose | - |
Ogataea angusta | ADP + D-xylulose 5-phosphate | - |
r | |
ATP + D-xylulose | - |
Escherichia coli DH5-alpha | ADP + D-xylulose 5-phosphate | - |
r |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 38 | - |
Ogataea angusta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |