Literature summary for 2.7.1.17 extracted from

Jin, Y.S.; Jones, S.; Shi, N.Q.; Jeffries, T.W.
Molecular cloning of XYL3 (D-xylulokinase) from Pichia stipitis and characterization of its physiological function (2002), Appl. Environ. Microbiol., 68, 1232-1239.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli. Expression of XYL3 using its Pichia stipitis promoter increase Saccharomyces cerevisiae D-xylulose consumption 3fold and enables the transformants to produce ethanol from a mixture of xylulose and xylose, whereas the parental strain only accumulates xylitol. In vitro, D-xylulokinase activity in recombinant Saccharomyces cerevisiae is sixfold higher with a multicopy than with a single-copy XYL3 plasmid, but ethanol production decreases with increased copy number	Scheffersomyces stipitis

Cloned (Comment)

Organism

expression in Escherichia coli. Expression of XYL3 using its Pichia stipitis promoter increase Saccharomyces cerevisiae D-xylulose consumption 3fold and enables the transformants to produce ethanol from a mixture of xylulose and xylose, whereas the parental strain only accumulates xylitol. In vitro, D-xylulokinase activity in recombinant Saccharomyces cerevisiae is sixfold higher with a multicopy than with a single-copy XYL3 plasmid, but ethanol production decreases with increased copy number

Scheffersomyces stipitis

Organism

Organism	UniProt	Comment	Textmining
Scheffersomyces stipitis	-	CBS 6054	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-xylulose	-	Scheffersomyces stipitis	ADP + D-xylulose 5-phosphate	-	r

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Release 2023.1 (January 2023)