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Literature summary for 2.7.1.17 extracted from

  • Stumpf, P.K.; Horecker, B.L.
    The role of xylulose 5-phosphate in xylose metabolism of lactobacillus pentosus (1956), J. Biol. Chem., 218, 753-768.
    View publication on PubMed
Search for more literature for 2.7.1.17

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ activation, better than Mg2+ Lactiplantibacillus pentosus
Mg2+ activation, equally effective as Mn2+ or Zn2+ Lactiplantibacillus pentosus
Mn2+ activation is as effective as with Mg2+, equally effective as Zn2+ or Mg2+ Lactiplantibacillus pentosus
Zn2+ activation, equally effective as Mg2+ or Mn2+ Lactiplantibacillus pentosus

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus pentosus
-
-
-
Lactiplantibacillus pentosus 124-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Lactiplantibacillus pentosus

Storage Stability

Storage Stability Organism
0°C, several months Lactiplantibacillus pentosus
4°C, 1 mg protein/ml in 0.1 M potassium phosphate buffer, pH 7, 2 mM MgCl2, 0.1 mM EDTA, 0.1 mM PMSF, 10 mM 2-mercaptoethanol, 90% loss of activity within 18 h Lactiplantibacillus pentosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-xylulose specific for D-isomer Lactiplantibacillus pentosus ADP + D-xylulose 5-phosphate
-
 r
ATP + D-xylulose specific for D-isomer Lactiplantibacillus pentosus 124-2 ADP + D-xylulose 5-phosphate
-
 r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53
-
 5 min, 66%, 60%, 50%, 30% or 26% loss of activity at pH 8, pH 7, pH 6.2, pH 5.7 and pH 5.4, respectively Lactiplantibacillus pentosus
60
-
 5 min, inactivation at pH 5.4 Lactiplantibacillus pentosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.9
-
 Lactiplantibacillus pentosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.4
-
 5 min, 26% loss of activity at 53°C, inactivation at 60°C Lactiplantibacillus pentosus
5.7
-
 5 min, 30% loss of activity at 53°C Lactiplantibacillus pentosus
6.2
-
 5 min, 50% loss of activity at 53°C Lactiplantibacillus pentosus
7
-
 5 min, 60% loss of activity at 53°C Lactiplantibacillus pentosus
8
-
 5 min, 66% loss of activity at 53°C Lactiplantibacillus pentosus