BRENDA - Enzyme Database
show all sequences of 2.7.1.169

Identification of pantoate kinase and phosphopantothenate synthetase from Methanospirillum hungatei

Katoh, H.; Tamaki, H.; Tokutake, Y.; Hanada, S.; Chohnan, S.; J. Biosci. Bioeng. 115, 372-376 (2013)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Methanospirillum hungatei
Inhibitors
Inhibitors
Commentary
Organism
Structure
malonyl-CoA
0.2 mM, 83% residual activity
Methanospirillum hungatei
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
Co2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
Fe2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
Mg2+
maximum activity in presence of Mg2+
Methanospirillum hungatei
Mn2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
additional information
not accepted: Cu2+
Methanospirillum hungatei
Ni2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
Zn2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
Organism
Organism
UniProt
Commentary
Textmining
Methanospirillum hungatei
Q2FUB2
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + (R)-pantoate
-
722842
Methanospirillum hungatei
ADP + (R)-phosphopantoate
-
-
-
?
UTP + (R)-pantoate
52.7% of the activity with ATP
722842
Methanospirillum hungatei
UDP + (R)-phosphopantoate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
COG1829
-
Methanospirillum hungatei
Mhun_0831
-
Methanospirillum hungatei
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Methanospirillum hungatei
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
60
-
no residual activity
Methanospirillum hungatei
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Methanospirillum hungatei
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
9.5
more than 80% of maximum activity
Methanospirillum hungatei
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Methanospirillum hungatei
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
malonyl-CoA
0.2 mM, 83% residual activity
Methanospirillum hungatei
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
Co2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
Fe2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
Mg2+
maximum activity in presence of Mg2+
Methanospirillum hungatei
Mn2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
additional information
not accepted: Cu2+
Methanospirillum hungatei
Ni2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
Zn2+
broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+
Methanospirillum hungatei
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + (R)-pantoate
-
722842
Methanospirillum hungatei
ADP + (R)-phosphopantoate
-
-
-
?
UTP + (R)-pantoate
52.7% of the activity with ATP
722842
Methanospirillum hungatei
UDP + (R)-phosphopantoate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Methanospirillum hungatei
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
60
-
no residual activity
Methanospirillum hungatei
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Methanospirillum hungatei
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
9.5
more than 80% of maximum activity
Methanospirillum hungatei
General Information
General Information
Commentary
Organism
physiological function
coexpression of genes Mhun_0831 and Mhun_0832 complements the poor growth of the temperature-sensitive Escherichia coli pantothenate kinase mutant ts9. The recombinant Mhun_0831 and Mhun_0832 genes expressed in Escherichia coli cells exhibit pantoate kinase and phosphopantothenate synthetase activities, respectively
Methanospirillum hungatei
General Information (protein specific)
General Information
Commentary
Organism
physiological function
coexpression of genes Mhun_0831 and Mhun_0832 complements the poor growth of the temperature-sensitive Escherichia coli pantothenate kinase mutant ts9. The recombinant Mhun_0831 and Mhun_0832 genes expressed in Escherichia coli cells exhibit pantoate kinase and phosphopantothenate synthetase activities, respectively
Methanospirillum hungatei
Other publictions for EC 2.7.1.169
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722842
Katoh
Identification of pantoate kin ...
Methanospirillum hungatei
J. Biosci. Bioeng.
115
372-376
2013
-
-
1
-
-
-
1
-
-
8
-
-
-
15
-
-
-
-
-
-
-
-
2
-
2
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
8
-
-
-
-
-
-
-
-
-
-
2
-
1
1
-
-
1
1
-
-
-
1
1
-
-
-
722549
Tomita
Biochemical characterization o ...
Thermococcus kodakarensis
J. Bacteriol.
194
5434-5443
2012
-
-
1
-
7
-
2
5
-
2
-
-
-
9
-
-
-
-
-
-
-
-
5
-
2
1
-
-
5
1
-
-
-
1
-
-
-
-
1
-
-
7
-
-
2
1
5
-
2
-
-
-
-
-
-
-
-
-
-
5
-
1
-
-
5
1
-
-
-
-
-
-
-
4
4
704547
Yokooji
Pantoate kinase and phosphopan ...
Thermococcus kodakarensis
J. Biol. Chem.
284
28137-28145
2009
-
-
-
-
-
-
2
3
-
-
2
1
-
5
-
-
-
-
-
-
-
-
3
2
2
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
2
1
-
-
-
-
-
-
-
-
3
2
1
-
-
3
-
-
-
-
-
1
1
-
3
3