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Literature summary for 2.7.1.169 extracted from

  • Yokooji, Y.; Tomita, H.; Atomi, H.; Imanaka, T.
    Pantoate kinase and phosphopantothenate synthetase, two novel enzymes necessary for CoA biosynthesis in the archaea (2009), J. Biol. Chem., 284, 28137-28145.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
CoA no inhibition in presence of 0.1 mM CoA, activity decrases by 20% in presence of 1 mM CoA Thermococcus kodakarensis
additional information no detectable decrase in activity at 0.1 mM: acetyl-CoA, dephospho-CoA, or 4'-phosphopantothenate Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
ATP 42°C Thermococcus kodakarensis
1.2
-
Pantoate 42°C Thermococcus kodakarensis
1.3
-
pantothenate 42°C Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32748
-
2 * 32748, calculated from sequence Thermococcus kodakarensis
34000
-
2 * 34000, SDS-PAGE Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pantoate Thermococcus kodakarensis
-
ADP + 4-phosphopantoate
-
?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JHF1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pantoate
-
Thermococcus kodakarensis ADP + 4-phosphopantoate
-
?
ATP + pantoate kcat/Km value with pantoate as a substrate is over 7fold higher than that observed with pantothenate Thermococcus kodakarensis ADP + 4-phosphopantoate
-
?
ATP + pantothenate kcat/Km value with pantoate as a substrate is over 7fold higher than that observed with pantothenate Thermococcus kodakarensis ADP + 4'-phosphopantothenate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Thermococcus kodakarensis
dimer 2 * 32748, calculated from sequence Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
PoK
-
Thermococcus kodakarensis
TK2141 protein
-
Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
assay Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.21
-
pantothenate 42°C Thermococcus kodakarensis
1.48
-
ATP 42°C Thermococcus kodakarensis
1.56
-
Pantoate 42°C Thermococcus kodakarensis

General Information

General Information Comment Organism
malfunction the deletion mutant strain grows well in media supplemented with 1 mM CoA. The deletion mutant strain could not grow at all in the absence of CoA Thermococcus kodakarensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.17
-
pantothenate 42°C Thermococcus kodakarensis
1.3
-
Pantoate 42°C Thermococcus kodakarensis
3.1
-
ATP 42°C Thermococcus kodakarensis