BRENDA - Enzyme Database
show all sequences of 2.7.1.169

Pantoate kinase and phosphopantothenate synthetase, two novel enzymes necessary for CoA biosynthesis in the archaea

Yokooji, Y.; Tomita, H.; Atomi, H.; Imanaka, T.; J. Biol. Chem. 284, 28137-28145 (2009)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CoA
no inhibition in presence of 0.1 mM CoA, activity decrases by 20% in presence of 1 mM CoA
Thermococcus kodakarensis
additional information
no detectable decrase in activity at 0.1 mM: acetyl-CoA, dephospho-CoA, or 4'-phosphopantothenate
Thermococcus kodakarensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.47
-
ATP
42°C
Thermococcus kodakarensis
1.2
-
Pantoate
42°C
Thermococcus kodakarensis
1.3
-
pantothenate
42°C
Thermococcus kodakarensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32748
-
2 * 32748, calculated from sequence
Thermococcus kodakarensis
34000
-
2 * 34000, SDS-PAGE
Thermococcus kodakarensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + pantoate
Thermococcus kodakarensis
-
ADP + 4-phosphopantoate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Thermococcus kodakarensis
Q5JHF1
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + pantoate
-
704547
Thermococcus kodakarensis
ADP + 4-phosphopantoate
-
-
-
?
ATP + pantoate
kcat/Km value with pantoate as a substrate is over 7fold higher than that observed with pantothenate
704547
Thermococcus kodakarensis
ADP + 4-phosphopantoate
-
-
-
?
ATP + pantothenate
kcat/Km value with pantoate as a substrate is over 7fold higher than that observed with pantothenate
704547
Thermococcus kodakarensis
ADP + 4'-phosphopantothenate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 34000, SDS-PAGE
Thermococcus kodakarensis
dimer
2 * 32748, calculated from sequence
Thermococcus kodakarensis
Synonyms
Synonyms
Commentary
Organism
PoK
-
Thermococcus kodakarensis
TK2141 protein
-
Thermococcus kodakarensis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
42
-
assay
Thermococcus kodakarensis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.21
-
pantothenate
42°C
Thermococcus kodakarensis
1.48
-
ATP
42°C
Thermococcus kodakarensis
1.56
-
Pantoate
42°C
Thermococcus kodakarensis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CoA
no inhibition in presence of 0.1 mM CoA, activity decrases by 20% in presence of 1 mM CoA
Thermococcus kodakarensis
additional information
no detectable decrase in activity at 0.1 mM: acetyl-CoA, dephospho-CoA, or 4'-phosphopantothenate
Thermococcus kodakarensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.47
-
ATP
42°C
Thermococcus kodakarensis
1.2
-
Pantoate
42°C
Thermococcus kodakarensis
1.3
-
pantothenate
42°C
Thermococcus kodakarensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32748
-
2 * 32748, calculated from sequence
Thermococcus kodakarensis
34000
-
2 * 34000, SDS-PAGE
Thermococcus kodakarensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + pantoate
Thermococcus kodakarensis
-
ADP + 4-phosphopantoate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + pantoate
-
704547
Thermococcus kodakarensis
ADP + 4-phosphopantoate
-
-
-
?
ATP + pantoate
kcat/Km value with pantoate as a substrate is over 7fold higher than that observed with pantothenate
704547
Thermococcus kodakarensis
ADP + 4-phosphopantoate
-
-
-
?
ATP + pantothenate
kcat/Km value with pantoate as a substrate is over 7fold higher than that observed with pantothenate
704547
Thermococcus kodakarensis
ADP + 4'-phosphopantothenate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 34000, SDS-PAGE
Thermococcus kodakarensis
dimer
2 * 32748, calculated from sequence
Thermococcus kodakarensis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
42
-
assay
Thermococcus kodakarensis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.21
-
pantothenate
42°C
Thermococcus kodakarensis
1.48
-
ATP
42°C
Thermococcus kodakarensis
1.56
-
Pantoate
42°C
Thermococcus kodakarensis
General Information
General Information
Commentary
Organism
malfunction
the deletion mutant strain grows well in media supplemented with 1 mM CoA. The deletion mutant strain could not grow at all in the absence of CoA
Thermococcus kodakarensis
General Information (protein specific)
General Information
Commentary
Organism
malfunction
the deletion mutant strain grows well in media supplemented with 1 mM CoA. The deletion mutant strain could not grow at all in the absence of CoA
Thermococcus kodakarensis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.17
-
pantothenate
42°C
Thermococcus kodakarensis
1.3
-
Pantoate
42°C
Thermococcus kodakarensis
3.1
-
ATP
42°C
Thermococcus kodakarensis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.17
-
pantothenate
42°C
Thermococcus kodakarensis
1.3
-
Pantoate
42°C
Thermococcus kodakarensis
3.1
-
ATP
42°C
Thermococcus kodakarensis
Other publictions for EC 2.7.1.169
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722842
Katoh
Identification of pantoate kin ...
Methanospirillum hungatei
J. Biosci. Bioeng.
115
372-376
2013
-
-
1
-
-
-
1
-
-
8
-
-
-
15
-
-
-
-
-
-
-
-
2
-
2
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
8
-
-
-
-
-
-
-
-
-
-
2
-
1
1
-
-
1
1
-
-
-
1
1
-
-
-
722549
Tomita
Biochemical characterization o ...
Thermococcus kodakarensis
J. Bacteriol.
194
5434-5443
2012
-
-
1
-
7
-
2
5
-
2
-
-
-
9
-
-
-
-
-
-
-
-
5
-
2
1
-
-
5
1
-
-
-
1
-
-
-
-
1
-
-
7
-
-
2
1
5
-
2
-
-
-
-
-
-
-
-
-
-
5
-
1
-
-
5
1
-
-
-
-
-
-
-
4
4
704547
Yokooji
Pantoate kinase and phosphopan ...
Thermococcus kodakarensis
J. Biol. Chem.
284
28137-28145
2009
-
-
-
-
-
-
2
3
-
-
2
1
-
5
-
-
-
-
-
-
-
-
3
2
2
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
2
1
-
-
-
-
-
-
-
-
3
2
1
-
-
3
-
-
-
-
-
1
1
-
3
3