Application | Comment | Organism |
---|---|---|
synthesis | a microbial production platform in Escherichia coli to synthesize D-glyceric acid from D-galacturonate represents an alternative for the production of D-glyceric acid, an industrially relevant chemical, that addresses current challenges in using acetic acid bacteria for its synthesis: increasing yield, enantio-purity and biological stability, overview | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | development of a microbial production platform in Escherichia coli to synthesize D-glyceric acid from D-galacturonate. The expression of uronate dehydrogenase (udh) from Pseudomonas syringae and galactarolactone isomerase (gli) from Agrobacterium fabrum, along with the inactivation of garK, encoding for glycerate kinase, enables D-glyceric acid accumulation by utilizing the endogenous expression of galactarate dehydratase (garD), 5-keto-4-deoxy-D-glucarate aldolase (garL), and 2-hydroxy-3-oxopropionate reductase (garR). Optimization of carbon flux through the elimination of competing metabolic pathways leads to the development of a DELTAgarKDELTAhyiDELTAglxKDELTAuxaC mutant strain that produces 4.8 g/l of D-glyceric acid from D-galacturonate, with an 83% molar yield. Cultivation in a minimal medium produces similar yields and demonstrates that galactose or glycerol serve as possible carbon co-feeds for industrial production, method overview | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glycerate | Escherichia coli | - |
ADP + 2-phospho-(R)-glycerate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P23524 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glycerate | - |
Escherichia coli | ADP + 2-phospho-(R)-glycerate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
garK | - |
Escherichia coli |
glycerate kinase | - |
Escherichia coli |