BRENDA - Enzyme Database show
show all sequences of 2.7.1.165

Characterization of glycerate kinase (2-phosphoglycerate forming), a key enzyme of the nonphosphorylative Entner-Doudoroff pathway, from the thermoacidophilic euryarchaeon Picrophilus torridus

Reher, M.; Bott, M.; Schoenheit, P.; FEMS Microbiol. Lett. 259, 113-119 (2006)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.34
-
(R)-glycerate
-
Picrophilus torridus
0.5
-
ATP
-
Picrophilus torridus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
0.05 mM, most effective divalent cation
Picrophilus torridus
additional information
Mg2+ can be replaced to some extent by Ni2+ (25%), Mn2+ (11%), and Co2+ (11%); no activating effect of K+
Picrophilus torridus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47500
-
2 * 47500, gel filtration
Picrophilus torridus
50000
-
SDS-PAGE, denatured enzyme
Picrophilus torridus
93000
-
calculated from amino acid sequence
Picrophilus torridus
95000
-
gel filtration, native enzyme
Picrophilus torridus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Picrophilus torridus
-
-
-
Purification (Commentary)
Commentary
Organism
Resource Phenyl column chromatography, Superdex 200 HiLoad 16/60 column gel filtration, and UNO-Q1 column chromatography
Picrophilus torridus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.07
-
cell extract, at 50°C
Picrophilus torridus
358
-
after 5114fold purification, at 50°C
Picrophilus torridus
Storage Stability
Storage Stability
Organism
60°C, 100 mM Tris-HCl at pH 8.0, 2 h, no loss of activity
Picrophilus torridus
70°C, 100 mM Tris-HCl at pH 8.0, 5 min, 50% loss of activity
Picrophilus torridus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (R)-glycerate
-
673730
Picrophilus torridus
ADP + 3-phospho-(R)-glycerate
-
-
-
?
additional information
the enzyme shows highest activity with D-glycerate (100%), and lower activity (34%) with L-glycerate
673730
Picrophilus torridus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 47500, gel filtration
Picrophilus torridus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Picrophilus torridus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
-
Picrophilus torridus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.6
8.6
50% of activity at pH 5.6 and at pH 8.6
Picrophilus torridus
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Picrophilus torridus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Picrophilus torridus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.34
-
(R)-glycerate
-
Picrophilus torridus
0.5
-
ATP
-
Picrophilus torridus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
0.05 mM, most effective divalent cation
Picrophilus torridus
additional information
Mg2+ can be replaced to some extent by Ni2+ (25%), Mn2+ (11%), and Co2+ (11%); no activating effect of K+
Picrophilus torridus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47500
-
2 * 47500, gel filtration
Picrophilus torridus
50000
-
SDS-PAGE, denatured enzyme
Picrophilus torridus
93000
-
calculated from amino acid sequence
Picrophilus torridus
95000
-
gel filtration, native enzyme
Picrophilus torridus
Purification (Commentary) (protein specific)
Commentary
Organism
Resource Phenyl column chromatography, Superdex 200 HiLoad 16/60 column gel filtration, and UNO-Q1 column chromatography
Picrophilus torridus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.07
-
cell extract, at 50°C
Picrophilus torridus
358
-
after 5114fold purification, at 50°C
Picrophilus torridus
Storage Stability (protein specific)
Storage Stability
Organism
60°C, 100 mM Tris-HCl at pH 8.0, 2 h, no loss of activity
Picrophilus torridus
70°C, 100 mM Tris-HCl at pH 8.0, 5 min, 50% loss of activity
Picrophilus torridus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (R)-glycerate
-
673730
Picrophilus torridus
ADP + 3-phospho-(R)-glycerate
-
-
-
?
additional information
the enzyme shows highest activity with D-glycerate (100%), and lower activity (34%) with L-glycerate
673730
Picrophilus torridus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 47500, gel filtration
Picrophilus torridus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Picrophilus torridus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
-
Picrophilus torridus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.6
8.6
50% of activity at pH 5.6 and at pH 8.6
Picrophilus torridus
Other publictions for EC 2.7.1.165
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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722247
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Unraveling the function of the ...
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1
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1
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6
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722379
Sass
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Homo sapiens
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31
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1
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2
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1
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1
1
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-
696906
Liu
A MOFRL family glycerate kinas ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
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20
1
1
1
1
-
1
1
-
1
-
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-
1
1
-
-
1
-
13
-
-
-
8
2
2
-
-
-
1
-
-
-
-
20
1
1
1
1
-
1
1
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-
686769
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Only plant-type (GLYK) glycera ...
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6
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3
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672800
Kehrer
Glycerate kinase of the hypert ...
Thermoproteus tenax
BMC Genet.
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2007
1
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2
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2
3
-
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3
1
1
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2
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1
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3
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1
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1
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2
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Liu
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Pyrococcus horikoshii
Extremophiles
11
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2007
1
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-
-
3
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9
4
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5
2
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1
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13
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1
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2
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5
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6
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5
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1
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1
13
2
2
2
2
3
2
1
1
-
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-
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-
685823
Kehrer
Glycerate kinase of the hypert ...
Thermoproteus tenax
BMC Genomics
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2007
-
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1
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-
3
2
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6
2
1
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1
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7
1
1
1
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2
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1
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2
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1
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7
1
1
1
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2
-
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-
672720
Noh
-
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11
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-
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1
3
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4
5
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42
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1
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5
1
3
2
4
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7
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3
5
2
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2
4
-
2
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673730
Reher
Characterization of glycerate ...
Picrophilus torridus
FEMS Microbiol. Lett.
259
113-119
2006
-
-
-
-
-
-
-
2
-
2
4
-
-
6
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1
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2
2
2
1
1
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1
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4
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1
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749246
Schwarzenbacher
Crystal structure of a glycer ...
Thermotoga maritima, Thermotoga maritima DSM 3109
Proteins
65
243-248
2006
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699594
Sims
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Hubbard
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641336
Hill
The purification of glycerate ...
Hyphomicrobium sp., Pseudomonas sp.
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