BRENDA - Enzyme Database show
show all sequences of 2.7.1.165

Purification and characterization of glycerate kinase from a serine-producing methylotroph, Hyphomicrobium methylovorum GM2

Yoshida, T.; Fukuta, K.; Mitsunaga, T.; Yamada, H.; Izumi, Y.; Eur. J. Biochem. 210, 849-854 (1992)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2,3-diphospho-D-glycerate
-
Hyphomicrobium methylovorum
3-phosphoglycerate
-
Hyphomicrobium methylovorum
AgNO3
-
Hyphomicrobium methylovorum
CuCl2
-
Hyphomicrobium methylovorum
HgCl2
-
Hyphomicrobium methylovorum
Hydroxypyruvate
-
Hyphomicrobium methylovorum
L-Glycerate
-
Hyphomicrobium methylovorum
p-chloromercuribenzoate
-
Hyphomicrobium methylovorum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
ATP
cosubstrate D-glycerate, at pH 7.0, 30C
Hyphomicrobium methylovorum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
10 mM, 75% of the activity with Mg2+
Hyphomicrobium methylovorum
K+
50 mM, high stimulation
Hyphomicrobium methylovorum
Mg2+
10 mM, activates; required
Hyphomicrobium methylovorum
Mn2+
10 mM, 72% of the activity with 10 mM Mg2+
Hyphomicrobium methylovorum
NH4+
50 mM, high stimulation
Hyphomicrobium methylovorum
Ni2+
stimulates, 10 mM, 29% of the activity with Mg2+
Hyphomicrobium methylovorum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
48000
gel filtration
Hyphomicrobium methylovorum
52000
-
1 * 52000, SDS-PAGE
Hyphomicrobium methylovorum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hyphomicrobium methylovorum
-
GM2
-
Purification (Commentary)
Commentary
Organism
-
Hyphomicrobium methylovorum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
181
-
pH 7.0, 30C
Hyphomicrobium methylovorum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (R)-glycerate
activity towards L-glycerate is 13% of that towards D-glycerate
641338
Hyphomicrobium methylovorum
ADP + 3-phospho-(R)-glycerate
2-phosphoglycerate
641338
Hyphomicrobium methylovorum
ir
CTP + (R)-glycerate
at 59% of the activity with ATP
641338
Hyphomicrobium methylovorum
CDP + 3-phospho-(R)-glycerate
-
-
-
?
GTP + (R)-glycerate
at 59% of the activity with ATP
641338
Hyphomicrobium methylovorum
GDP + 3-phospho-(R)-glycerate
-
-
-
?
UTP + (R)-glycerate
at 64% of the activity with ATP
641338
Hyphomicrobium methylovorum
UDP + 3-phospho-(R)-glycerate
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 52000, SDS-PAGE
Hyphomicrobium methylovorum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
-
Hyphomicrobium methylovorum
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
35
-
30 min, stable
Hyphomicrobium methylovorum
40
-
30 min, 14.3% loss of activity
Hyphomicrobium methylovorum
50
-
30 min, 52.4% loss of activity
Hyphomicrobium methylovorum
60
-
30 min, 87.3% loss of activity
Hyphomicrobium methylovorum
70
-
30 min, 100% loss of activity
Hyphomicrobium methylovorum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Hyphomicrobium methylovorum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
10
30C, 30 min, stable
Hyphomicrobium methylovorum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,3-diphospho-D-glycerate
-
Hyphomicrobium methylovorum
3-phosphoglycerate
-
Hyphomicrobium methylovorum
AgNO3
-
Hyphomicrobium methylovorum
CuCl2
-
Hyphomicrobium methylovorum
HgCl2
-
Hyphomicrobium methylovorum
Hydroxypyruvate
-
Hyphomicrobium methylovorum
L-Glycerate
-
Hyphomicrobium methylovorum
p-chloromercuribenzoate
-
Hyphomicrobium methylovorum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
ATP
cosubstrate D-glycerate, at pH 7.0, 30C
Hyphomicrobium methylovorum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
10 mM, 75% of the activity with Mg2+
Hyphomicrobium methylovorum
K+
50 mM, high stimulation
Hyphomicrobium methylovorum
Mg2+
10 mM, activates; required
Hyphomicrobium methylovorum
Mn2+
10 mM, 72% of the activity with 10 mM Mg2+
Hyphomicrobium methylovorum
NH4+
50 mM, high stimulation
Hyphomicrobium methylovorum
Ni2+
stimulates, 10 mM, 29% of the activity with Mg2+
Hyphomicrobium methylovorum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
48000
gel filtration
Hyphomicrobium methylovorum
52000
-
1 * 52000, SDS-PAGE
Hyphomicrobium methylovorum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Hyphomicrobium methylovorum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
181
-
pH 7.0, 30C
Hyphomicrobium methylovorum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (R)-glycerate
activity towards L-glycerate is 13% of that towards D-glycerate
641338
Hyphomicrobium methylovorum
ADP + 3-phospho-(R)-glycerate
2-phosphoglycerate
641338
Hyphomicrobium methylovorum
ir
CTP + (R)-glycerate
at 59% of the activity with ATP
641338
Hyphomicrobium methylovorum
CDP + 3-phospho-(R)-glycerate
-
-
-
?
GTP + (R)-glycerate
at 59% of the activity with ATP
641338
Hyphomicrobium methylovorum
GDP + 3-phospho-(R)-glycerate
-
-
-
?
UTP + (R)-glycerate
at 64% of the activity with ATP
641338
Hyphomicrobium methylovorum
UDP + 3-phospho-(R)-glycerate
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 52000, SDS-PAGE
Hyphomicrobium methylovorum
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
-
Hyphomicrobium methylovorum
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
35
-
30 min, stable
Hyphomicrobium methylovorum
40
-
30 min, 14.3% loss of activity
Hyphomicrobium methylovorum
50
-
30 min, 52.4% loss of activity
Hyphomicrobium methylovorum
60
-
30 min, 87.3% loss of activity
Hyphomicrobium methylovorum
70
-
30 min, 100% loss of activity
Hyphomicrobium methylovorum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Hyphomicrobium methylovorum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
10
30C, 30 min, stable
Hyphomicrobium methylovorum
Other publictions for EC 2.7.1.165
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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739413
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e0122957
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722247
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1
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2
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1
3
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5
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1
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1
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6
1
1
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4
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1
1
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722379
Sass
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Homo sapiens
Hum. Mutat.
31
1280-1285
2010
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1
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3
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2
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1
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1
1
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696906
Liu
A MOFRL family glycerate kinas ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
Biotechnol. Lett.
31
1937-1941
2009
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-
1
-
-
1
13
-
-
8
2
2
-
2
-
-
1
-
-
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-
20
1
1
1
1
-
1
1
-
1
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1
1
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1
-
13
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8
2
2
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1
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20
1
1
1
1
-
1
1
-
-
-
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-
-
-
-
686769
Bartsch
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582
3025-3028
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1
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6
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3
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3
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672800
Kehrer
Glycerate kinase of the hypert ...
Thermoproteus tenax
BMC Genet.
8
301
2007
1
-
1
-
-
-
3
2
-
2
3
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-
1
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1
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3
1
1
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2
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1
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1
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3
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3
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1
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3
1
1
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2
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673501
Liu
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Pyrococcus horikoshii
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11
733-739
2007
1
-
1
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3
3
-
9
4
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5
2
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1
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13
1
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2
2
3
1
1
1
1
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1
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4
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14
6
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1
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1
13
2
2
2
2
3
2
1
1
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685823
Kehrer
Glycerate kinase of the hypert ...
Thermoproteus tenax
BMC Genomics
8
301
2007
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1
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3
2
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6
2
1
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7
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7
1
1
1
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2
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672720
Noh
-
Purification and characterizat ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2, Thermoplasma acidophilum
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11
344-350
2006
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1
3
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4
5
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42
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5
1
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2
4
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2
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7
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3
5
2
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2
4
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2
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673730
Reher
Characterization of glycerate ...
Picrophilus torridus
FEMS Microbiol. Lett.
259
113-119
2006
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4
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749246
Schwarzenbacher
Crystal structure of a glycer ...
Thermotoga maritima, Thermotoga maritima DSM 3109
Proteins
65
243-248
2006
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699594
Sims
-
Reed, G.H.: Method for the enz ...
Escherichia coli
J. Mol. Catal. B
32
77-81
2005
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5083
Hubbard
Evolution of enzymatic activit ...
Escherichia coli
Biochemistry
37
14369-14375
1998
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641338
Yoshida
Purification and characterizat ...
Hyphomicrobium methylovorum
Eur. J. Biochem.
210
849-854
1992
-
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8
1
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6
2
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1
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1
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1
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4
1
1
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5
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1
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1
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8
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1
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6
2
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1
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4
1
1
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5
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1
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1
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-
641336
Hill
The purification of glycerate ...
Hyphomicrobium sp., Pseudomonas sp.
J. Gen. Microbiol.
83
187-190
1974
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