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Literature summary for 2.7.1.163 extracted from
- Stable genetic transformation of castor (Ricinus communis L.) via particle gun-mediated gene transfer using embryo axes from mature seeds (2008), Plant Cell Rep., 27, 1509-1519.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | used as selectable marker gene | Escherichia coli |
| molecular biology | resistance against hyromycin B mediated by transformation of the hph gene | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Physical parameters for transient transformation are optimized using the UidA gene encoding beta-glucuronidase as the reporter gene and with hygromycin-phosphotransferase (hptII) gene as selectable marker | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
Binary vector pCAMBIA-1305.2 contains hphII gene desenced from Escherichia coli, transformed via Agrobacterium tumefaciens | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | Seeds cultivar DCS-9 (Jyoti) of Ricinus communis L. are used for gun-mediated gene transfer. The cultivars 48-1 and VP-1 are also used for particle bombardement. | Escherichia coli | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hptII | - |
Escherichia coli |
| hygromycin-phosphotransferase | - |
Escherichia coli |
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