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Literature summary for 2.7.1.163 extracted from

  • Rao, R.N.; Allen, N.E.; Hobbs, J.N.; Alborn, W.E.; Kirst, H.A.; Paschal, J.W.
    Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli (1983), Antimicrob. Agents Chemother., 24, 689-695.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.163

Application

Application Comment Organism
molecular biology Used as marker gene mediating hygromycin resistance. Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
hygromycin B resistance gene is cloned in pBR322, recombinant plasmids pKC241 pKC222 carrying the resistance Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ included in assay medium Escherichia coli
NH4+ included in assay medium Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + hygromycin B Escherichia coli phosphorylated product procures hygromcin B resistance ADP + 4-O-phosphohygromycin B
-
 ir
additional information Escherichia coli no phosphorylation of neomycin, kanamycin A and B, streptomycin, dideoxykanamycin B, tobramycin, gentamicin, G418, sisomicin, netilmicin, amikacin, apramycin, ribostamycin, butirosin, lividomycin, and paromomycin by this enzyme ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 used strains are K-12 derivatives, isolating of a plasmid conferring resistance to the aminocyclitol antibiotic hygromycin B
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture strains W677, BE783, BE827, DH1, BE1065, BE1092, and BE1098 are used Escherichia coli
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 107471 counts/minute/25 microl sample extract, hygromycin as substrate Escherichia coli
additional information
-
 14328 counts/minute/25 microl sample extract, A23444 as substrate Escherichia coli
additional information
-
 15508 counts/minute/25 microl sample extract, SS-56C as substrate Escherichia coli
additional information
-
 24483 counts/minute/25 microl sample extract, destomycin A as substrate Escherichia coli
additional information
-
 35387 counts/minute/25 microl sample extract, strain BE1065 containing plasmid pKC222 Escherichia coli
additional information
-
 50051 counts/minute/25 microl sample extract, strain BE1098 containing plasmid pKC241 Escherichia coli
additional information
-
 5986 counts/minute/25 microl sample extract, hygromycin b2 as substrate Escherichia coli
additional information
-
 8428 counts/minute/25 microl sample extract, destomycin B as substrate Escherichia coli
additional information
-
 hygromycin B is an aminocyclitol antibiotic with broad-spectrum activity against both procaryotic and eucaryotic cells Escherichia coli
additional information
-
 No activity is found in the plasmid-free DH1 strain. Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + destomycin A 1-N-methyl isomer of hygromycin B Escherichia coli ADP + 4-O-phosphodestomycin A
-
 ?
ATP + destomycin B 1-N-methyl-4',4"-epi hygromycin B Escherichia coli ADP + 4-O-phosphodestomycin B
-
 ?
ATP + hygromycin B phosphorylated product procures hygromcin B resistance Escherichia coli ADP + 4-O-phosphohygromycin B
-
 ir
ATP + hygromycin B2 pseudodisaccharide of D-talose and hyosamine Escherichia coli ADP + 4-O-phosphohygromycin B2
-
 ?
additional information no phosphorylation of neomycin, kanamycin A and B, streptomycin, dideoxykanamycin B, tobramycin, gentamicin, G418, sisomicin, netilmicin, amikacin, apramycin, ribostamycin, butirosin, lividomycin, and paromomycin by this enzyme Escherichia coli ?
-
 ?
additional information the analogs of destomycin A and B, A23444 (N-demethyl hygromycin B) and SS-56C (N-demethyl-2-hydroxy hygromycin B) with modifications in the cyclitol ring moiety, are phosphoryted by the enzyme Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
Hm phosphotransferase
-
 Escherichia coli
hygromycin B4-O-phosphohygromycin
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli