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Literature summary for 2.7.1.162 extracted from

  • Guan, W.; Bai, J.; Zhou, T.; Zhao, B.
    Active sites of N-acetylhexosamine 1-kinase from Bifidobacterium longum (2016), Wei Sheng Wu Xue Bao, 56, 68-77 .
No PubMed abstract available
Search for more literature for 2.7.1.162

Protein Variants

Protein Variants Comment Organism
D208A site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type Bifidobacterium longum subsp. Longum
D208E site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type Bifidobacterium longum subsp. Longum
D208N site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type Bifidobacterium longum subsp. Longum
F247A site-directed mutagenesis, the mutant shows altered pH optimum of pH 7.0 as compared to wild-type optimum of pH 7.5 Bifidobacterium longum subsp. Longum
H31A site-directed mutagenesis, the mutant shows altered pH optimum of pH 7.0 as compared to wild-type optimum of pH 7.5 Bifidobacterium longum subsp. Longum
H31V site-directed mutagenesis, the mutant shows altered pH optimum of pH 7.0 as compared to wild-type optimum of pH 7.5 Bifidobacterium longum subsp. Longum
I24A site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type Bifidobacterium longum subsp. Longum
I24V site-directed mutagenesis, the mutant shows altered pH optimum of pH 7.0 as compared to wild-type optimum of pH 7.5 Bifidobacterium longum subsp. Longum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for catalysis, optimum for wild-type enzyme is 5 mM, for mutants H31A and F247A 10 mM Bifidobacterium longum subsp. Longum

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum subsp. Longum E8MF12
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Bifidobacterium longum subsp. Longum ATCC 15707 E8MF12
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Bifidobacterium longum subsp. Longum DSM 20219 E8MF12
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-
Bifidobacterium longum subsp. Longum E194b E8MF12
-
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Bifidobacterium longum subsp. Longum JCM 1217 E8MF12
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Bifidobacterium longum subsp. Longum NCTC 11818 E8MF12
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-

Synonyms

Synonyms Comment Organism
BLLJ_1622 locus name Bifidobacterium longum subsp. Longum
NahK
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 Bifidobacterium longum subsp. Longum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 mutant enzymes H31A, H31V, F247A, and I24V Bifidobacterium longum subsp. Longum
7.5
-
 wild-type enzyme Bifidobacterium longum subsp. Longum