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Literature summary for 2.7.1.162 extracted from

  • Li, Y.; Yu, H.; Chen, Y.; Lau, K.; Cai, L.; Cao, H.; Tiwari, V.; Qu, J.; Thon, V.; Wang, P.; Chen, X.
    Substrate promiscuity of N-acetylhexosamine 1-kinases (2011), Molecules, 16, 6396-6407.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.162

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bifidobacterium longum subsp. infantis
expression in Escherichia coli Bifidobacterium longum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 N-acetyl-alpha-D-glucosamine pH 8.0, 37°C Bifidobacterium longum subsp. infantis
0.06
-
 N-acetyl-alpha-D-glucosamine pH 8.0, 37°C Bifidobacterium longum
0.06
-
 ATP cosubstrate N-acetyl-alpha-D-galactosamine, pH 8.0, 37°C Bifidobacterium longum
0.08
-
 N-acetyl-alpha-D-galactosamine pH 8.0, 37°C Bifidobacterium longum
0.08
-
 ATP cosubstrate N-acetyl-alpha-D-galactosamine, pH 8.0, 37°C Bifidobacterium longum subsp. infantis
0.09
-
 N-acetyl-alpha-D-galactosamine pH 8.0, 37°C Bifidobacterium longum subsp. infantis
0.1
-
 ATP cosubstrate N-acetyl-alpha-D-glucosamine, pH 8.0, 37°C Bifidobacterium longum subsp. infantis
0.11
-
 ATP cosubstrate N-acetyl-alpha-D-glucosamine, pH 8.0, 37°C Bifidobacterium longum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal concentration 1mM Bifidobacterium longum subsp. infantis
Mg2+ optimal concentration 1mM Bifidobacterium longum
additional information divalent cation required for activity Bifidobacterium longum subsp. infantis
additional information divalent cation required for activity Bifidobacterium longum

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum
-
-
-
Bifidobacterium longum ATCC 55813
-
-
-
Bifidobacterium longum subsp. infantis
-
-
-
Bifidobacterium longum subsp. infantis ATCC 15697
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-2-azido-D-mannose 0.75 microM, 40.2% conversion Bifidobacterium longum ADP + 2-deoxy-2-azido-alpha-D-mannose 1-phosphate
-
 ?
ATP + 2-deoxy-2-azido-D-mannose 0.75 microM, 53.3% conversion Bifidobacterium longum subsp. infantis ADP + 2-deoxy-2-azido-alpha-D-mannose 1-phosphate
-
 ?
ATP + 2-deoxy-2-azido-D-mannose 0.75 microM, 53.3% conversion Bifidobacterium longum subsp. infantis ATCC 15697 ADP + 2-deoxy-2-azido-alpha-D-mannose 1-phosphate
-
 ?
ATP + 2-deoxy-2-fluoro-D-mannose 0.75 microM, 44.4% conversion Bifidobacterium longum subsp. infantis ADP + 2-deoxy-2-fluoro-alpha-D-mannose 1-phosphate
-
 ?
ATP + 2-deoxy-2-fluoro-D-mannose 0.75 microM, 47.0% conversion Bifidobacterium longum ADP + 2-deoxy-2-fluoro-alpha-D-mannose 1-phosphate
-
 ?
ATP + 2-deoxy-D-glucose 0.75 microM, 28.4% conversion Bifidobacterium longum ADP + 2-deoxy-alpha-D-glucose 1-phosphate
-
 ?
ATP + 2-deoxy-D-glucose 0.75 microM, 44.8% conversion Bifidobacterium longum subsp. infantis ADP + 2-deoxy-alpha-D-glucose 1-phosphate
-
 ?
ATP + 4-deoxy-D-mannose 0.75 microM, 23.9% conversion Bifidobacterium longum ADP + 4-deoxy-alpha-D-mannose 1-phosphate
-
 ?
ATP + 4-deoxy-D-mannose 0.75 microM, 37.1% conversion Bifidobacterium longum subsp. infantis ADP + 4-deoxy-alpha-D-mannose 1-phosphate
-
 ?
ATP + 6-deoxy-6-azido-N-acetyl-D-glucosamine 0.75 microM, 23.4% conversion Bifidobacterium longum ADP + 6-deoxy-6-azido-N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
ATP + 6-deoxy-6-azido-N-acetyl-D-glucosamine 0.75 microM, 37.2% conversion Bifidobacterium longum subsp. infantis ADP + 6-deoxy-6-azido-N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
ATP + 6-deoxy-6-azido-N-acetyl-D-glucosamine 0.75 microM, 37.2% conversion Bifidobacterium longum subsp. infantis ATCC 15697 ADP + 6-deoxy-6-azido-N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
ATP + 6-deoxy-6-azido-N-acetyl-D-glucosamine 0.75 microM, 23.4% conversion Bifidobacterium longum ATCC 55813 ADP + 6-deoxy-6-azido-N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
ATP + 6-deoxy-6-methyl-N-acetyl-D-glucosamine 0.75 microM, 36.3% conversion Bifidobacterium longum ADP + 6-deoxy-6-methyl-N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
ATP + 6-deoxy-6-methyl-N-acetyl-D-glucosamine 0.75 microM, 41.8% conversion Bifidobacterium longum subsp. infantis ADP + 6-deoxy-6-methyl-N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
ATP + 6-methyl-N-acetyl-D-mannosamine 0.75 microM, 28.6% conversion Bifidobacterium longum ADP + 6-methyl-N-acetyl-alpha-D-mannosamine 1-phosphate
-
 ?
ATP + 6-methyl-N-acetyl-D-mannosamine 0.75 microM, 32.6% conversion Bifidobacterium longum subsp. infantis ADP + 6-methyl-N-acetyl-alpha-D-mannosamine 1-phosphate
-
 ?
ATP + D-mannose 0.75 microM, 37.1% conversion Bifidobacterium longum ADP + alpha-D-mannose 1-phosphate
-
 ?
ATP + D-mannose 0.75 microM, 68.0% conversion Bifidobacterium longum subsp. infantis ADP + alpha-D-mannose 1-phosphate
-
 ?
ATP + N-acetyl-alpha-D-galactosamine
-
 Bifidobacterium longum subsp. infantis ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
ATP + N-acetyl-alpha-D-galactosamine
-
 Bifidobacterium longum ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
ATP + N-acetyl-alpha-D-galactosamine
-
 Bifidobacterium longum subsp. infantis ATCC 15697 ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
ATP + N-acetyl-alpha-D-galactosamine
-
 Bifidobacterium longum ATCC 55813 ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
ATP + N-acetyl-alpha-D-glucosamine 0.75 microM, 35.4% conversion Bifidobacterium longum subsp. infantis ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
ATP + N-acetyl-alpha-D-glucosamine 0.75 microM, 42.3% conversion Bifidobacterium longum ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
ATP + N-acetyl-alpha-D-glucosamine 0.75 microM, 35.4% conversion Bifidobacterium longum subsp. infantis ATCC 15697 ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
ATP + N-acetyl-alpha-D-glucosamine 0.75 microM, 42.3% conversion Bifidobacterium longum ATCC 55813 ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
ATP + N-benzoyl-alpha-D-galactosamine 0.75 microM, 51.9% conversion Bifidobacterium longum ADP + N-benzoyl-alpha-D-galactosamine 1-phosphate
-
 ?
ATP + N-benzoyl-alpha-D-galactosamine 0.75 microM, 62.2% conversion Bifidobacterium longum subsp. infantis ADP + N-benzoyl-alpha-D-galactosamine 1-phosphate
-
 ?
ATP + N-butanoyl-D-galactosamine 0.75 microM, 24.0% conversion Bifidobacterium longum ADP + N-butanoyl-alpha-D-galactosamine 1-phosphate
-
 ?
ATP + N-butanoyl-D-galactosamine 0.75 microM, 24.0% conversion Bifidobacterium longum ATCC 55813 ADP + N-butanoyl-alpha-D-galactosamine 1-phosphate
-
 ?
ATP + N-butanoyl-D-glucosamine 0.75 microM, 20.9% conversion Bifidobacterium longum subsp. infantis ADP + N-butanoyl-alpha-D-glucosamine 1-phosphate
-
 ?
ATP + N-butanoyl-D-glucosamine 0.75 microM, 35.0% conversion Bifidobacterium longum ADP + N-butanoyl-alpha-D-glucosamine 1-phosphate
-
 ?
ATP + N-butanoyl-D-glucosamine 0.75 microM, 20.9% conversion Bifidobacterium longum subsp. infantis ATCC 15697 ADP + N-butanoyl-alpha-D-glucosamine 1-phosphate
-
 ?
ATP + N-butanoyl-D-glucosamine 0.75 microM, 35.0% conversion Bifidobacterium longum ATCC 55813 ADP + N-butanoyl-alpha-D-glucosamine 1-phosphate
-
 ?
ATP + N-trifluoroacetyl-alpha-D-galactosamine 0.75 microM, 21.8% conversion Bifidobacterium longum ADP + N-trifluoroacetyl-alpha-D-galactosamine 1-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
NahK
-
 Bifidobacterium longum subsp. infantis
NahK
-
 Bifidobacterium longum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
 ATP cosubstrate N-acetyl-alpha-D-galactosamine, pH 8.0, 37°C Bifidobacterium longum subsp. infantis
0.46
-
 N-acetyl-alpha-D-galactosamine pH 8.0, 37°C Bifidobacterium longum subsp. infantis
0.48
-
 ATP cosubstrate N-acetyl-alpha-D-galactosamine, pH 8.0, 37°C Bifidobacterium longum
0.57
-
 N-acetyl-alpha-D-galactosamine pH 8.0, 37°C Bifidobacterium longum
0.95
-
 N-acetyl-alpha-D-glucosamine pH 8.0, 37°C Bifidobacterium longum subsp. infantis
1.1
-
 N-acetyl-alpha-D-glucosamine pH 8.0, 37°C Bifidobacterium longum
1.1
-
 ATP cosubstrate N-acetyl-alpha-D-glucosamine, pH 8.0, 37°C Bifidobacterium longum subsp. infantis
1.3
-
 ATP cosubstrate N-acetyl-alpha-D-glucosamine, pH 8.0, 37°C Bifidobacterium longum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Bifidobacterium longum subsp. infantis
7 8
-
 Bifidobacterium longum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.8
-
 ATP cosubstrate N-acetyl-alpha-D-galactosamine, pH 8.0, 37°C Bifidobacterium longum subsp. infantis
5.1
-
 N-acetyl-alpha-D-galactosamine pH 8.0, 37°C Bifidobacterium longum subsp. infantis
7.1
-
 N-acetyl-alpha-D-galactosamine pH 8.0, 37°C Bifidobacterium longum
8
-
 ATP cosubstrate N-acetyl-alpha-D-galactosamine, pH 8.0, 37°C Bifidobacterium longum
11
-
 ATP cosubstrate N-acetyl-alpha-D-glucosamine, pH 8.0, 37°C Bifidobacterium longum subsp. infantis
11.8
-
 ATP cosubstrate N-acetyl-alpha-D-glucosamine, pH 8.0, 37°C Bifidobacterium longum
15.8
-
 N-acetyl-alpha-D-glucosamine pH 8.0, 37°C Bifidobacterium longum subsp. infantis
18.3
-
 N-acetyl-alpha-D-glucosamine pH 8.0, 37°C Bifidobacterium longum