BRENDA - Enzyme Database show
show all sequences of 2.7.1.162

A chemoenzymatic route to N-acetylglucosamine-1-phosphate analogues: substrate specificity investigations of N-acetylhexosamine 1-kinase

Cai, L.; Guan, W.; Kitaoka, M.; Shen, J.; Xia, C.; Chen, W.; Wang, P.G.; Chem. Commun. (Camb. ) 2009, 2944-2946 (2009)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + N-acetyl-alpha-D-glucosamine
Bifidobacterium longum
-
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bifidobacterium longum
E8MF12
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2-(acetylamino)-2-deoxy-D-allopyranose
22% phosphorylation yield
703179
Bifidobacterium longum
ADP + 2-(acetylamino)-2-deoxy-D-allopyranose 1-phosphate
-
-
-
?
ATP + 6-deoxy-6-azido-N-acetyl-D-glucosamine
34% phosphorylation yield
703179
Bifidobacterium longum
ADP + 6-deoxy-6-azido-N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + 6-deoxy-N-acetyl-D-glucosamine
75% phosphorylation yield
703179
Bifidobacterium longum
ADP + 6-deoxy-N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-glucosamine
-
703179
Bifidobacterium longum
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-D-galactosamine
78% phosphorylation yield
703179
Bifidobacterium longum
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
90% phosphorylation yield
703179
Bifidobacterium longum
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-azidoacetyl-D-glucosamine
87% phosphorylation yield
703179
Bifidobacterium longum
ADP + N-azidoacetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-butanoyl-D-glucosamine
87% phosphorylation yield
703179
Bifidobacterium longum
ADP + N-butanoyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-phenylacetyl-D-glucosamine
88% phosphorylation yield
703179
Bifidobacterium longum
ADP + N-phenylacetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-propionyl-D-glucosamine
86% phosphorylation yield
703179
Bifidobacterium longum
ADP + N-propionyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
additional information
enzyme demonstrates a relaxed specificity when modifications are of the N-acyl type but also shows a much more limited tolerance for 6-modifications, N-acetylglucosamine 3- and 4-epimers can also be recognized by this enzyme
703179
Bifidobacterium longum
?
-
-
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + N-acetyl-alpha-D-glucosamine
Bifidobacterium longum
-
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2-(acetylamino)-2-deoxy-D-allopyranose
22% phosphorylation yield
703179
Bifidobacterium longum
ADP + 2-(acetylamino)-2-deoxy-D-allopyranose 1-phosphate
-
-
-
?
ATP + 6-deoxy-6-azido-N-acetyl-D-glucosamine
34% phosphorylation yield
703179
Bifidobacterium longum
ADP + 6-deoxy-6-azido-N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + 6-deoxy-N-acetyl-D-glucosamine
75% phosphorylation yield
703179
Bifidobacterium longum
ADP + 6-deoxy-N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-glucosamine
-
703179
Bifidobacterium longum
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-D-galactosamine
78% phosphorylation yield
703179
Bifidobacterium longum
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-D-glucosamine
90% phosphorylation yield
703179
Bifidobacterium longum
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-azidoacetyl-D-glucosamine
87% phosphorylation yield
703179
Bifidobacterium longum
ADP + N-azidoacetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-butanoyl-D-glucosamine
87% phosphorylation yield
703179
Bifidobacterium longum
ADP + N-butanoyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-phenylacetyl-D-glucosamine
88% phosphorylation yield
703179
Bifidobacterium longum
ADP + N-phenylacetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-propionyl-D-glucosamine
86% phosphorylation yield
703179
Bifidobacterium longum
ADP + N-propionyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
additional information
enzyme demonstrates a relaxed specificity when modifications are of the N-acyl type but also shows a much more limited tolerance for 6-modifications, N-acetylglucosamine 3- and 4-epimers can also be recognized by this enzyme
703179
Bifidobacterium longum
?
-
-
-
-
Other publictions for EC 2.7.1.162
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739523
Li
Efficient chemoenzymatic synth ...
Escherichia coli
Prep. Biochem. Biotechnol.
47
852-859
2017
-
1
1
-
-
-
-
-
-
-
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1
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2
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1
1
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-
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-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737770
Sato
Open-close structural change u ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217
Biochim. Biophys. Acta
1854
333-340
2015
-
-
1
1
4
-
-
8
-
1
-
2
-
5
-
-
1
1
-
-
-
-
4
2
1
-
-
8
1
-
-
-
-
-
-
-
-
1
-
1
4
-
-
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8
-
1
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2
-
-
-
1
-
-
-
-
4
2
1
-
-
8
1
-
-
-
-
1
1
-
-
-
737343
Wang
Insights into the binding spec ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217, Bifidobacterium longum subsp. infantis, Bifidobacterium longum subsp. infantis ATCC 15697
Acta Crystallogr. Sect. D
70
1401-1410
2014
-
-
2
2
17
-
-
1
-
2
2
3
-
12
-
-
2
2
-
-
-
-
24
3
2
-
-
-
2
-
-
2
-
-
-
-
-
2
2
2
17
-
-
-
-
1
-
2
2
3
-
-
-
2
-
-
-
-
24
3
2
-
-
-
2
-
-
-
-
4
4
-
-
-
726089
Li
Efficient enzymatic synthesis ...
Bifidobacterium longum subsp. infantis
Org. Lett.
15
5528-5530
2013
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
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-
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-
-
-
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-
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-
-
-
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-
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-
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-
-
-
-
723238
Li
Substrate promiscuity of N-ace ...
Bifidobacterium longum, Bifidobacterium longum ATCC 55813, Bifidobacterium longum subsp. infantis, Bifidobacterium longum subsp. infantis ATCC 15697
Molecules
16
6396-6407
2011
-
-
2
-
-
-
-
8
-
4
-
-
-
11
-
-
-
-
-
-
-
-
36
-
-
-
-
8
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
8
-
4
-
-
-
-
-
-
-
-
-
-
36
-
-
-
-
8
2
-
-
-
-
-
-
-
8
8
702588
Cai
Substrate specificity of N-ace ...
Bifidobacterium longum
Bioorg. Med. Chem. Lett.
19
5433-5435
2009
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
703179
Cai
A chemoenzymatic route to N-ac ...
Bifidobacterium longum
Chem. Commun. (Camb. )
2009
2944-2946
2009
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
11
-
-
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-
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-
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-
1
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-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677700
Nishimoto
Identification of N-acetylhexo ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217
Appl. Environ. Microbiol.
73
6444-6449
2007
1
-
1
-
-
1
-
3
-
6
-
6
-
7
-
-
1
1
-
1
13
-
17
-
1
-
1
3
1
-
1
3
-
-
-
1
-
1
3
-
-
1
-
-
-
3
-
6
-
6
-
-
-
1
-
1
13
-
17
-
1
-
1
3
1
-
1
-
-
-
-
-
-
-