BRENDA - Enzyme Database
show all sequences of 2.7.1.162

Substrate specificity of N-acetylhexosamine kinase towards N-acetylgalactosamine derivatives

Cai, L.; Guan, W.; Wang, W.; Zhao, W.; Kitaoka, M.; Shen, J.; ONeil, C.; Wang, P.G.; Bioorg. Med. Chem. Lett. 19, 5433-5435 (2009)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
MgCl2
10 mM are included in the assay medium
Bifidobacterium longum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + N-acetyl-alpha-D-glucosamine
Bifidobacterium longum
-
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Bifidobacterium longum
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + 4- deoxy-N-acetyl-D-glucosamine
50% phosphorylation yield
702588
Bifidobacterium longum
ADP + 4-deoxy-N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + 4-deoxy-4-azido-N-acetyl-D-galactosamine
73% phosphorylation yield
702588
Bifidobacterium longum
ADP + 4-deoxy-4-azido-N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + 6-deoxy-6-azido-N-acetyl-D-galactosamine
42% phosphorylation yield
702588
Bifidobacterium longum
ADP + 6-deoxy-6-azido-N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + 6-deoxy-N-acetyl-D-galactosamine
37% phosphorylation yield
702588
Bifidobacterium longum
ADP + 6-deoxy-N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + D-galactopyranose
below 5% phosphorylation yield
702588
Bifidobacterium longum
ADP + alpha-D-galactopyranose 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-glucosamine
-
702588
Bifidobacterium longum
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-D-galactosamine
78% phosphorylation yield
702588
Bifidobacterium longum
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-azidoacetyl-D-galactosamine
65% phosphorylation yield
702588
Bifidobacterium longum
ADP + N-azidoacetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-butanoyl-D-galactosamine
86% phosphorylation yield
702588
Bifidobacterium longum
ADP + N-butanoyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-phenylacetyl-D-galactosamine
77% phosphorylation yield
702588
Bifidobacterium longum
ADP + N-phenylacetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-propionyl-D-galactosamine
85% phosphorylation yield
702588
Bifidobacterium longum
ADP + N-propionyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
N-acetylhexosamine kinase
-
Bifidobacterium longum
NahK
-
Bifidobacterium longum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bifidobacterium longum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
assay at
Bifidobacterium longum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
MgCl2
10 mM are included in the assay medium
Bifidobacterium longum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + N-acetyl-alpha-D-glucosamine
Bifidobacterium longum
-
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + 4- deoxy-N-acetyl-D-glucosamine
50% phosphorylation yield
702588
Bifidobacterium longum
ADP + 4-deoxy-N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + 4-deoxy-4-azido-N-acetyl-D-galactosamine
73% phosphorylation yield
702588
Bifidobacterium longum
ADP + 4-deoxy-4-azido-N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + 6-deoxy-6-azido-N-acetyl-D-galactosamine
42% phosphorylation yield
702588
Bifidobacterium longum
ADP + 6-deoxy-6-azido-N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + 6-deoxy-N-acetyl-D-galactosamine
37% phosphorylation yield
702588
Bifidobacterium longum
ADP + 6-deoxy-N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + D-galactopyranose
below 5% phosphorylation yield
702588
Bifidobacterium longum
ADP + alpha-D-galactopyranose 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-glucosamine
-
702588
Bifidobacterium longum
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-D-galactosamine
78% phosphorylation yield
702588
Bifidobacterium longum
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-azidoacetyl-D-galactosamine
65% phosphorylation yield
702588
Bifidobacterium longum
ADP + N-azidoacetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-butanoyl-D-galactosamine
86% phosphorylation yield
702588
Bifidobacterium longum
ADP + N-butanoyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-phenylacetyl-D-galactosamine
77% phosphorylation yield
702588
Bifidobacterium longum
ADP + N-phenylacetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-propionyl-D-galactosamine
85% phosphorylation yield
702588
Bifidobacterium longum
ADP + N-propionyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bifidobacterium longum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
assay at
Bifidobacterium longum
Other publictions for EC 2.7.1.162
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739523
Li
Efficient chemoenzymatic synth ...
Escherichia coli
Prep. Biochem. Biotechnol.
47
852-859
2017
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737770
Sato
Open-close structural change u ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217
Biochim. Biophys. Acta
1854
333-340
2015
-
-
1
1
4
-
-
8
-
1
-
2
-
5
-
-
1
1
-
-
-
-
4
2
3
1
-
-
8
1
-
-
-
-
-
-
-
-
1
-
1
4
-
-
-
-
8
-
1
-
2
-
-
-
1
-
-
-
-
4
2
1
-
-
8
1
-
-
-
-
1
1
-
-
-
737343
Wang
Insights into the binding spec ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217, Bifidobacterium longum subsp. infantis, Bifidobacterium longum subsp. infantis ATCC 15697
Acta Crystallogr. Sect. D
70
1401-1410
2014
-
-
2
2
17
-
-
1
-
2
2
3
-
12
-
-
2
2
-
-
-
-
24
3
7
2
-
-
-
2
-
-
2
-
-
-
-
-
2
2
2
17
-
-
-
-
1
-
2
2
3
-
-
-
2
-
-
-
-
24
3
2
-
-
-
2
-
-
-
-
4
4
-
-
-
726089
Li
Efficient enzymatic synthesis ...
Bifidobacterium longum subsp. infantis
Org. Lett.
15
5528-5530
2013
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723238
Li
Substrate promiscuity of N-ace ...
Bifidobacterium longum, Bifidobacterium longum ATCC 55813, Bifidobacterium longum subsp. infantis, Bifidobacterium longum subsp. infantis ATCC 15697
Molecules
16
6396-6407
2011
-
-
2
-
-
-
-
8
-
4
-
-
-
11
-
-
-
-
-
-
-
-
36
-
2
-
-
-
8
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
8
-
4
-
-
-
-
-
-
-
-
-
-
36
-
-
-
-
8
2
-
-
-
-
-
-
-
8
8
702588
Cai
Substrate specificity of N-ace ...
Bifidobacterium longum
Bioorg. Med. Chem. Lett.
19
5433-5435
2009
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
11
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
703179
Cai
A chemoenzymatic route to N-ac ...
Bifidobacterium longum
Chem. Commun. (Camb. )
2009
2944-2946
2009
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
11
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677700
Nishimoto
Identification of N-acetylhexo ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217
Appl. Environ. Microbiol.
73
6444-6449
2007
1
-
1
-
-
1
-
3
-
6
-
6
-
7
-
-
1
1
-
1
13
-
17
-
4
1
-
1
3
1
-
1
3
-
-
-
1
-
1
3
-
-
1
-
-
-
3
-
6
-
6
-
-
-
1
-
1
13
-
17
-
1
-
1
3
1
-
1
-
-
-
-
-
-
-