BRENDA - Enzyme Database
show all sequences of 2.7.1.162

Identification of N-acetylhexosamine 1-kinase in the complete lacto-N-biose I/galacto-N-biose metabolic pathway in Bifidobacterium longum

Nishimoto, M.; Kitaoka, M.; Appl. Environ. Microbiol. 73, 6444-6449 (2007)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
isopropyl-1-thio-beta-D-galactoside
induction of protein expression at a final concentration of 0.5 mM
Bifidobacterium longum
Cloned(Commentary)
Cloned (Commentary)
Organism
Escherichia coli strains select96 and BL21(DE3) are used as hosts for cloning and expression.; expression in Escherichia coli
Bifidobacterium longum
General Stability
General Stability
Organism
loses half of enzyme activity during incubation for 30 min at 50°C in 0.1 M Tris buffer (pH 8.5)
Bifidobacterium longum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
N-acetyl-alpha-D-galactosamine
; pH 8.5, 30°C
Bifidobacterium longum
0.118
-
N-acetyl-alpha-D-glucosamine
; pH 8.5, 30°C
Bifidobacterium longum
0.172
-
ATP
at N-acetyl-alpha-D-glucosamine saturation; cosubstrate: N-acetyl-alpha-D-glucosamine, pH 8.5, 30°C
Bifidobacterium longum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
1 mM, 1% relative activity compared to Mg2+
Bifidobacterium longum
Co2+
1 mM, 21% relative activity compared to Mg2+; 21% of the activity with Mg2+
Bifidobacterium longum
Mg2+
1 mM, 100% relative activity; requires a divalent cation, with Mg2+ resulting in the greatest stimulation of enzyme activity
Bifidobacterium longum
Mn2+
1 mM, 7% relative activity compared to Mg2+
Bifidobacterium longum
Ni2+
1 mM, 4% relative activity compared to Mg2+
Bifidobacterium longum
Zn2+
1 mM, 8% relative activity compared to Mg2+
Bifidobacterium longum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + N-acetyl-alpha-D-galactosamine
Bifidobacterium longum
the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
Bifidobacterium longum
lacto-N-biose I/galacto-N-biose metabolic pathway
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
Bifidobacterium longum JCM 1217
the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
Bifidobacterium longum JCM 1217
lacto-N-biose I/galacto-N-biose metabolic pathway
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
?
ATP + N-acetyl-alpha-D-glucosamine
Bifidobacterium longum
-
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
ATP + N-acetyl-alpha-D-glucosamine
Bifidobacterium longum
the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Bifidobacterium longum
E8MF12
strain JCM1217
-
Bifidobacterium longum JCM 1217
E8MF12
strain JCM1217
-
Purification (Commentary)
Purification (Commentary)
Organism
; The transformant is cultivated and protein expression is induced by the addition of isopropyl-1-thio-beta-D-galactoside. The cells are harvested by centrifugation and sonicated. Cell debris is removed by centrifugation and enzyme is purified on an Ni-nitrilotriacetic acid agarose gel.
Bifidobacterium longum
Reaction
Reaction
Commentary
Organism
Reaction ID
ATP + N-acetyl-D-hexosamine = ADP + N-acetyl-alpha-D-hexosamine 1-phosphate
enzyme acts by a sequential bi bi (two substrates-two products) mechanism, with the reaction occurring after the binding of both ATP and N-acetylhexosamine
Bifidobacterium longum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
strain JCM1217
Bifidobacterium longum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
Incubation of 4 microM LnpB protein with 10 mM N-acetyl-alpha-D-glucosamine 6-sulfate in 0.1 M MOPS buffer (pH 7.5) at 30°C for 30 min shows that the protein have no hydrolytic activity
Bifidobacterium longum
0.001
-
D-glucose, substrate donor specificity
Bifidobacterium longum
0.002
-
D-mannosamine, substrate donor specificity
Bifidobacterium longum
0.004
-
D-glucosamine, substrate donor specificity
Bifidobacterium longum
0.007
-
2-deoxyglucose, substrate donor specificity
Bifidobacterium longum
0.024
-
D-mannose, substrate donor specificity
Bifidobacterium longum
0.049
-
D-talose, substrate donor specificity
Bifidobacterium longum
0.22
-
N-acetyl-D-mannosamine, substrate donor specificity
Bifidobacterium longum
0.48
-
ITP, substrate donor specificity
Bifidobacterium longum
0.65
-
GTP, substrate donor specificity
Bifidobacterium longum
0.89
-
N-acetyl-D-galactosamine, substrate donor specificity
Bifidobacterium longum
1.48
-
ATP, substrate donor specificity
Bifidobacterium longum
1.49
-
N-acetyl-D-glucosamine, substrate donor specificity
Bifidobacterium longum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + D-mannose
phosphorylation at 1.6% of the activity with N-acetylglucosamine
677700
Bifidobacterium longum
?
-
-
-
?
ATP + D-mannose
phosphorylation at 1.6% of the activity with N-acetylglucosamine
677700
Bifidobacterium longum JCM 1217
?
-
-
-
?
ATP + D-talose
phosphorylation at 3% of the activity with N-acetylglucosamine
677700
Bifidobacterium longum
?
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway
677700
Bifidobacterium longum
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
phosphorylation at 60% of the activity with N-acetylglucosamine
677700
Bifidobacterium longum
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
lacto-N-biose I/galacto-N-biose metabolic pathway
677700
Bifidobacterium longum
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway
677700
Bifidobacterium longum JCM 1217
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
phosphorylation at 60% of the activity with N-acetylglucosamine
677700
Bifidobacterium longum JCM 1217
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
lacto-N-biose I/galacto-N-biose metabolic pathway
677700
Bifidobacterium longum JCM 1217
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-glucosamine
-
677700
Bifidobacterium longum
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-glucosamine
the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway
677700
Bifidobacterium longum
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-D-mannosamine
phosphorylation at 15% of the activity with N-acetylglucosamine
677700
Bifidobacterium longum
?
-
-
-
?
ATP + N-acetyl-D-mannosamine
-
677700
Bifidobacterium longum
ADP + ?
-
-
-
?
GTP + N-acetyl-alpha-D-glucosamine
GTP shows about 45% of the activity with ATP
677700
Bifidobacterium longum
GDP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ITP + N-acetyl-alpha-D-glucosamine
ITP shows about 30% of the activity with ATP
677700
Bifidobacterium longum
IDP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
additional information
slightly active with D-talose, D-mannose, GTP, ITP
677700
Bifidobacterium longum
?
-
-
-
-
additional information
slightly active with D-talose, D-mannose, GTP, ITP
677700
Bifidobacterium longum JCM 1217
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
lnpB
-
Bifidobacterium longum
lnpB
protein
Bifidobacterium longum
N-acetylhexosamine 1-kinase
-
Bifidobacterium longum
NahK
-
Bifidobacterium longum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Bifidobacterium longum
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
pH 8.5, 30 min, 50% loss of activity
Bifidobacterium longum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.752
-
N-acetyl-alpha-D-galactosamine
-
Bifidobacterium longum
1.21
-
N-acetyl-alpha-D-glucosamine
; pH 8.5, 30°C
Bifidobacterium longum
6.08
-
N-acetyl-alpha-D-galactosamine
pH 8.5, 30°C
Bifidobacterium longum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
; assay at
Bifidobacterium longum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
9.5
stability at 30°C; stable
Bifidobacterium longum
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Bifidobacterium longum
GTP
-
Bifidobacterium longum
ITP
-
Bifidobacterium longum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
isopropyl-1-thio-beta-D-galactoside
induction of protein expression at a final concentration of 0.5 mM
Bifidobacterium longum
Cloned(Commentary) (protein specific)
Commentary
Organism
Escherichia coli strains select96 and BL21(DE3) are used as hosts for cloning and expression.; expression in Escherichia coli
Bifidobacterium longum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Bifidobacterium longum
GTP
-
Bifidobacterium longum
ITP
-
Bifidobacterium longum
General Stability (protein specific)
General Stability
Organism
loses half of enzyme activity during incubation for 30 min at 50°C in 0.1 M Tris buffer (pH 8.5)
Bifidobacterium longum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
N-acetyl-alpha-D-galactosamine
; pH 8.5, 30°C
Bifidobacterium longum
0.118
-
N-acetyl-alpha-D-glucosamine
; pH 8.5, 30°C
Bifidobacterium longum
0.172
-
ATP
at N-acetyl-alpha-D-glucosamine saturation; cosubstrate: N-acetyl-alpha-D-glucosamine, pH 8.5, 30°C
Bifidobacterium longum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
1 mM, 1% relative activity compared to Mg2+
Bifidobacterium longum
Co2+
1 mM, 21% relative activity compared to Mg2+; 21% of the activity with Mg2+
Bifidobacterium longum
Mg2+
1 mM, 100% relative activity; requires a divalent cation, with Mg2+ resulting in the greatest stimulation of enzyme activity
Bifidobacterium longum
Mn2+
1 mM, 7% relative activity compared to Mg2+
Bifidobacterium longum
Ni2+
1 mM, 4% relative activity compared to Mg2+
Bifidobacterium longum
Zn2+
1 mM, 8% relative activity compared to Mg2+
Bifidobacterium longum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + N-acetyl-alpha-D-galactosamine
Bifidobacterium longum
the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
Bifidobacterium longum
lacto-N-biose I/galacto-N-biose metabolic pathway
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
Bifidobacterium longum JCM 1217
the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
Bifidobacterium longum JCM 1217
lacto-N-biose I/galacto-N-biose metabolic pathway
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
?
ATP + N-acetyl-alpha-D-glucosamine
Bifidobacterium longum
-
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
ATP + N-acetyl-alpha-D-glucosamine
Bifidobacterium longum
the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
; The transformant is cultivated and protein expression is induced by the addition of isopropyl-1-thio-beta-D-galactoside. The cells are harvested by centrifugation and sonicated. Cell debris is removed by centrifugation and enzyme is purified on an Ni-nitrilotriacetic acid agarose gel.
Bifidobacterium longum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
strain JCM1217
Bifidobacterium longum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
Incubation of 4 microM LnpB protein with 10 mM N-acetyl-alpha-D-glucosamine 6-sulfate in 0.1 M MOPS buffer (pH 7.5) at 30°C for 30 min shows that the protein have no hydrolytic activity
Bifidobacterium longum
0.001
-
D-glucose, substrate donor specificity
Bifidobacterium longum
0.002
-
D-mannosamine, substrate donor specificity
Bifidobacterium longum
0.004
-
D-glucosamine, substrate donor specificity
Bifidobacterium longum
0.007
-
2-deoxyglucose, substrate donor specificity
Bifidobacterium longum
0.024
-
D-mannose, substrate donor specificity
Bifidobacterium longum
0.049
-
D-talose, substrate donor specificity
Bifidobacterium longum
0.22
-
N-acetyl-D-mannosamine, substrate donor specificity
Bifidobacterium longum
0.48
-
ITP, substrate donor specificity
Bifidobacterium longum
0.65
-
GTP, substrate donor specificity
Bifidobacterium longum
0.89
-
N-acetyl-D-galactosamine, substrate donor specificity
Bifidobacterium longum
1.48
-
ATP, substrate donor specificity
Bifidobacterium longum
1.49
-
N-acetyl-D-glucosamine, substrate donor specificity
Bifidobacterium longum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + D-mannose
phosphorylation at 1.6% of the activity with N-acetylglucosamine
677700
Bifidobacterium longum
?
-
-
-
?
ATP + D-mannose
phosphorylation at 1.6% of the activity with N-acetylglucosamine
677700
Bifidobacterium longum JCM 1217
?
-
-
-
?
ATP + D-talose
phosphorylation at 3% of the activity with N-acetylglucosamine
677700
Bifidobacterium longum
?
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway
677700
Bifidobacterium longum
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
phosphorylation at 60% of the activity with N-acetylglucosamine
677700
Bifidobacterium longum
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
lacto-N-biose I/galacto-N-biose metabolic pathway
677700
Bifidobacterium longum
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway
677700
Bifidobacterium longum JCM 1217
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
phosphorylation at 60% of the activity with N-acetylglucosamine
677700
Bifidobacterium longum JCM 1217
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
lacto-N-biose I/galacto-N-biose metabolic pathway
677700
Bifidobacterium longum JCM 1217
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-glucosamine
-
677700
Bifidobacterium longum
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-glucosamine
the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway
677700
Bifidobacterium longum
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-D-mannosamine
phosphorylation at 15% of the activity with N-acetylglucosamine
677700
Bifidobacterium longum
?
-
-
-
?
ATP + N-acetyl-D-mannosamine
-
677700
Bifidobacterium longum
ADP + ?
-
-
-
?
GTP + N-acetyl-alpha-D-glucosamine
GTP shows about 45% of the activity with ATP
677700
Bifidobacterium longum
GDP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ITP + N-acetyl-alpha-D-glucosamine
ITP shows about 30% of the activity with ATP
677700
Bifidobacterium longum
IDP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
additional information
slightly active with D-talose, D-mannose, GTP, ITP
677700
Bifidobacterium longum
?
-
-
-
-
additional information
slightly active with D-talose, D-mannose, GTP, ITP
677700
Bifidobacterium longum JCM 1217
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Bifidobacterium longum
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
pH 8.5, 30 min, 50% loss of activity
Bifidobacterium longum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.752
-
N-acetyl-alpha-D-galactosamine
-
Bifidobacterium longum
1.21
-
N-acetyl-alpha-D-glucosamine
; pH 8.5, 30°C
Bifidobacterium longum
6.08
-
N-acetyl-alpha-D-galactosamine
pH 8.5, 30°C
Bifidobacterium longum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
; assay at
Bifidobacterium longum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
9.5
stability at 30°C; stable
Bifidobacterium longum
Other publictions for EC 2.7.1.162
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739523
Li
Efficient chemoenzymatic synth ...
Escherichia coli
Prep. Biochem. Biotechnol.
47
852-859
2017
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737770
Sato
Open-close structural change u ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217
Biochim. Biophys. Acta
1854
333-340
2015
-
-
1
1
4
-
-
8
-
1
-
2
-
5
-
-
1
1
-
-
-
-
4
2
3
1
-
-
8
1
-
-
-
-
-
-
-
-
1
-
1
4
-
-
-
-
8
-
1
-
2
-
-
-
1
-
-
-
-
4
2
1
-
-
8
1
-
-
-
-
1
1
-
-
-
737343
Wang
Insights into the binding spec ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217, Bifidobacterium longum subsp. infantis, Bifidobacterium longum subsp. infantis ATCC 15697
Acta Crystallogr. Sect. D
70
1401-1410
2014
-
-
2
2
17
-
-
1
-
2
2
3
-
12
-
-
2
2
-
-
-
-
24
3
7
2
-
-
-
2
-
-
2
-
-
-
-
-
2
2
2
17
-
-
-
-
1
-
2
2
3
-
-
-
2
-
-
-
-
24
3
2
-
-
-
2
-
-
-
-
4
4
-
-
-
726089
Li
Efficient enzymatic synthesis ...
Bifidobacterium longum subsp. infantis
Org. Lett.
15
5528-5530
2013
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723238
Li
Substrate promiscuity of N-ace ...
Bifidobacterium longum, Bifidobacterium longum ATCC 55813, Bifidobacterium longum subsp. infantis, Bifidobacterium longum subsp. infantis ATCC 15697
Molecules
16
6396-6407
2011
-
-
2
-
-
-
-
8
-
4
-
-
-
11
-
-
-
-
-
-
-
-
36
-
2
-
-
-
8
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
8
-
4
-
-
-
-
-
-
-
-
-
-
36
-
-
-
-
8
2
-
-
-
-
-
-
-
8
8
702588
Cai
Substrate specificity of N-ace ...
Bifidobacterium longum
Bioorg. Med. Chem. Lett.
19
5433-5435
2009
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
11
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
703179
Cai
A chemoenzymatic route to N-ac ...
Bifidobacterium longum
Chem. Commun. (Camb. )
2009
2944-2946
2009
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
11
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677700
Nishimoto
Identification of N-acetylhexo ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217
Appl. Environ. Microbiol.
73
6444-6449
2007
1
-
1
-
-
1
-
3
-
6
-
6
-
7
-
-
1
1
-
1
13
-
17
-
4
1
-
1
3
1
-
1
3
-
-
-
1
-
1
3
-
-
1
-
-
-
3
-
6
-
6
-
-
-
1
-
1
13
-
17
-
1
-
1
3
1
-
1
-
-
-
-
-
-
-