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Literature summary for 2.7.1.160 extracted from
- Two-step mechanism and step-arrest mutants of Runella slithyformis NAD+-dependent tRNA 2'-phosphotransferase Tpt1 (2018), RNA, 24, 1144-1157 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the enzyme RslTpt1 is encoded within a putative RNA repair gene cluster, recombinant expression of wild-type RslTpt1 and mutant R16A/H17A/R64A/R119A RslTpt1 in Saccharomyces cerevisiae tpt1DELTA haploid strain YBS501, in which the TPT1 ORF is deleted and replaced by LEU2 and which is dependent for viability on the p360-TPT1 plasmid (CEN URA3 SceTPT1), recombinant expression of His6-tagged wild-type RslTpt1 and mutant R16A/H17A/R64A/R119A RslTpt1 in Escherichia coli strain L21(DE3)-CodonPlus | Runella slithyformis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R16A/H17A/R64A/R119A | site-directed mutagenesis, the R16A, H17A, R64A, and R119A mutations are deemed lethal in vivo | Runella slithyformis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-phospho-[ligated tRNA] + NAD+ | Runella slithyformis | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? |  |
| 2'-phospho-[ligated tRNA] + NAD+ | Runella slithyformis ATCC 49304 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Runella slithyformis | - |
- |
- |
| Runella slithyformis ATCC 49304 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His6-tagged wild-type RslTpt1 and mutant R16A/H17A/R64A/R119A RslTpt1 from Escherichia coli strain L21(DE3)-CodonPlus by nickel affinity chromatography, ultrafiltration, and gel filtration | Runella slithyformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Runella slithyformis | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Runella slithyformis ATCC 49304 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RslTpt1 | - |
Runella slithyformis |
| Tpt1 | - |
Runella slithyformis |
| tRNA 2'-phosphotransferase | - |
Runella slithyformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Runella slithyformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Runella slithyformis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Runella slithyformis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | enzyme Tpt1 catalyzes the transfer of an internal 2'-phosphate moiety (2'-PO4) from a branched 2'-phosphate RNA splice junction to NAD+ to form a clean 2'-hydroxy, 3'-5'-phosphodiester junction, ADP-ribose 1''-2'' cyclic phosphate, and nicotinamide. Recombinant expression of RslTpt1 in a yeast mutant complements a lethal tpt1DELTA knockout phenotype | Runella slithyformis |
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