Any feedback?
Literature summary for 2.7.1.160 extracted from
- NAD+-dependent synthesis of a 5-phospho-ADP-ribosylated RNA/DNA cap by RNA 2-phosphotransferase Tpt1 (2018), Nucleic Acids Res., 46, 9617-9624 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CTHT_0025470, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Thermochaetoides thermophila |
| gene kptA, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Acetivibrio thermocellus |
| gene kptA, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Aeropyrum pernix |
| gene kptA, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Pyrococcus horikoshii |
| gene kptA, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Archaeoglobus fulgidus |
| gene TPT1, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
| recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Runella slithyformis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R64A | inactive step 2-defective RslTpt1-R64A mutant | Runella slithyformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Acetivibrio thermocellus |  |
| EDTA | - |
Aeropyrum pernix | |
| EDTA | - |
Archaeoglobus fulgidus | |
| EDTA | - |
Homo sapiens | |
| EDTA | - |
Pyrococcus horikoshii | |
| EDTA | - |
Runella slithyformis | |
| EDTA | - |
Saccharomyces cerevisiae | |
| EDTA | - |
Thermochaetoides thermophila | |
| Formamide | - |
Acetivibrio thermocellus | |
| Formamide | - |
Aeropyrum pernix | |
| Formamide | - |
Archaeoglobus fulgidus | |
| Formamide | - |
Homo sapiens | |
| Formamide | - |
Pyrococcus horikoshii | |
| Formamide | - |
Runella slithyformis | |
| Formamide | - |
Saccharomyces cerevisiae | |
| Formamide | - |
Thermochaetoides thermophila | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic profile | Aeropyrum pernix |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-phospho-[ligated tRNA] + NAD+ | Homo sapiens | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Saccharomyces cerevisiae | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Runella slithyformis | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Acetivibrio thermocellus | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Thermochaetoides thermophila | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Aeropyrum pernix | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Pyrococcus horikoshii | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Archaeoglobus fulgidus | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Aeropyrum pernix ATCC 700893 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Aeropyrum pernix DSM 11879 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Thermochaetoides thermophila IMI 039719 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Acetivibrio thermocellus DSM 1237 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Archaeoglobus fulgidus NBRC 100126 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Archaeoglobus fulgidus ATCC 49558 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Thermochaetoides thermophila DSM 1495 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Pyrococcus horikoshii DSM 12428 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Acetivibrio thermocellus JCM 9322 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Acetivibrio thermocellus NBRC 103400 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Aeropyrum pernix JCM 9820 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Pyrococcus horikoshii NBRC 100139 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Pyrococcus horikoshii JCM 9974 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Acetivibrio thermocellus ATCC 27405 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Acetivibrio thermocellus VPI 7372 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Archaeoglobus fulgidus VC-16 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Thermochaetoides thermophila CBS 144.50 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Aeropyrum pernix NBRC 100138 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Pyrococcus horikoshii ATCC 700860 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Acetivibrio thermocellus NCIMB 10682 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Pyrococcus horikoshii OT-3 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Acetivibrio thermocellus NRRL B-4536 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | Archaeoglobus fulgidus JCM 9628 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | A3DJX6 | Clostridium thermocellum | - |
| Acetivibrio thermocellus ATCC 27405 | A3DJX6 | Clostridium thermocellum | - |
| Acetivibrio thermocellus DSM 1237 | A3DJX6 | Clostridium thermocellum | - |
| Acetivibrio thermocellus JCM 9322 | A3DJX6 | Clostridium thermocellum | - |
| Acetivibrio thermocellus NBRC 103400 | A3DJX6 | Clostridium thermocellum | - |
| Acetivibrio thermocellus NCIMB 10682 | A3DJX6 | Clostridium thermocellum | - |
| Acetivibrio thermocellus NRRL B-4536 | A3DJX6 | Clostridium thermocellum | - |
| Acetivibrio thermocellus VPI 7372 | A3DJX6 | Clostridium thermocellum | - |
| Aeropyrum pernix | Q9YFP5 | - |
- |
| Aeropyrum pernix ATCC 700893 | Q9YFP5 | - |
- |
| Aeropyrum pernix DSM 11879 | Q9YFP5 | - |
- |
| Aeropyrum pernix JCM 9820 | Q9YFP5 | - |
- |
| Aeropyrum pernix NBRC 100138 | Q9YFP5 | - |
- |
| Archaeoglobus fulgidus | O29841 | - |
- |
| Archaeoglobus fulgidus ATCC 49558 | O29841 | - |
- |
| Archaeoglobus fulgidus JCM 9628 | O29841 | - |
- |
| Archaeoglobus fulgidus NBRC 100126 | O29841 | - |
- |
| Archaeoglobus fulgidus VC-16 | O29841 | - |
- |
| Homo sapiens | Q86TN4 | - |
- |
| Pyrococcus horikoshii | O57899 | - |
- |
| Pyrococcus horikoshii ATCC 700860 | O57899 | - |
- |
| Pyrococcus horikoshii DSM 12428 | O57899 | - |
- |
| Pyrococcus horikoshii JCM 9974 | O57899 | - |
- |
| Pyrococcus horikoshii NBRC 100139 | O57899 | - |
- |
| Pyrococcus horikoshii OT-3 | O57899 | - |
- |
| Runella slithyformis | - |
- |
- |
| Saccharomyces cerevisiae | Q12272 | - |
- |
| Thermochaetoides thermophila | G0S5Z5 | - |
- |
| Thermochaetoides thermophila CBS 144.50 | G0S5Z5 | - |
- |
| Thermochaetoides thermophila DSM 1495 | G0S5Z5 | - |
- |
| Thermochaetoides thermophila IMI 039719 | G0S5Z5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Acetivibrio thermocellus |
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Thermochaetoides thermophila |
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Archaeoglobus fulgidus |
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Aeropyrum pernix |
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Pyrococcus horikoshii |
| recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Runella slithyformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Homo sapiens | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Saccharomyces cerevisiae | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Runella slithyformis | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Acetivibrio thermocellus | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Thermochaetoides thermophila | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Aeropyrum pernix | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Pyrococcus horikoshii | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Archaeoglobus fulgidus | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 13% conversion by the Archaeoglobus fulgidus enzyme | Archaeoglobus fulgidus | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 20% conversion by the Pyrococcus horikoshii enzyme | Pyrococcus horikoshii | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 21% conversion by the Chaetomium thermophilum enzyme | Thermochaetoides thermophila | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 98% conversion by ApeTpt1 | Aeropyrum pernix | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Aeropyrum pernix ATCC 700893 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 98% conversion by ApeTpt1 | Aeropyrum pernix ATCC 700893 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Aeropyrum pernix DSM 11879 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 98% conversion by ApeTpt1 | Aeropyrum pernix DSM 11879 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Thermochaetoides thermophila IMI 039719 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 21% conversion by the Chaetomium thermophilum enzyme | Thermochaetoides thermophila IMI 039719 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Acetivibrio thermocellus DSM 1237 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Archaeoglobus fulgidus NBRC 100126 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 13% conversion by the Archaeoglobus fulgidus enzyme | Archaeoglobus fulgidus NBRC 100126 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Archaeoglobus fulgidus ATCC 49558 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 13% conversion by the Archaeoglobus fulgidus enzyme | Archaeoglobus fulgidus ATCC 49558 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Thermochaetoides thermophila DSM 1495 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 21% conversion by the Chaetomium thermophilum enzyme | Thermochaetoides thermophila DSM 1495 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Pyrococcus horikoshii DSM 12428 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 20% conversion by the Pyrococcus horikoshii enzyme | Pyrococcus horikoshii DSM 12428 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Acetivibrio thermocellus JCM 9322 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Acetivibrio thermocellus NBRC 103400 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Aeropyrum pernix JCM 9820 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 98% conversion by ApeTpt1 | Aeropyrum pernix JCM 9820 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Pyrococcus horikoshii NBRC 100139 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 20% conversion by the Pyrococcus horikoshii enzyme | Pyrococcus horikoshii NBRC 100139 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Pyrococcus horikoshii JCM 9974 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 20% conversion by the Pyrococcus horikoshii enzyme | Pyrococcus horikoshii JCM 9974 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Acetivibrio thermocellus ATCC 27405 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Acetivibrio thermocellus VPI 7372 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Archaeoglobus fulgidus VC-16 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 13% conversion by the Archaeoglobus fulgidus enzyme | Archaeoglobus fulgidus VC-16 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Thermochaetoides thermophila CBS 144.50 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 21% conversion by the Chaetomium thermophilum enzyme | Thermochaetoides thermophila CBS 144.50 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Aeropyrum pernix NBRC 100138 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 98% conversion by ApeTpt1 | Aeropyrum pernix NBRC 100138 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Pyrococcus horikoshii ATCC 700860 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 20% conversion by the Pyrococcus horikoshii enzyme | Pyrococcus horikoshii ATCC 700860 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Acetivibrio thermocellus NCIMB 10682 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Pyrococcus horikoshii OT-3 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 20% conversion by the Pyrococcus horikoshii enzyme | Pyrococcus horikoshii OT-3 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Acetivibrio thermocellus NRRL B-4536 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | - |
Archaeoglobus fulgidus JCM 9628 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2'-phospho-[ligated tRNA] + NAD+ | 13% conversion by the Archaeoglobus fulgidus enzyme | Archaeoglobus fulgidus JCM 9628 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| additional information | enzyme ApeTpt1 catalyzes a one-step synthesis of a 5'-phospho-ADP-ribosylated cap structure (with no subsequent 5'-phosphotransferase step). ApeTpt1 caps RNA or DNA 5'-phosphate ends with ADP-ribose. pDNA and pRNA substrates are capped quantitatively at saturating enzyme in preence of NAD+. ApeTpt1 with 200 nM 10mer 5' 32P-labeled RNA or DNA oligonucleotides of identical nucleotide sequence (excepting T for U in DNA versus RNA) in the presence of 1 mM NAD+ resulted in the conversion of the substrates into RNA and DNA products of slower electrophoretic mobility. The 32P-labeled capped oligonucleotides are gel-purified and reacted with ApeTpt1 in the presence of 1 mM nicotinamide. This results in decapping of 20% of the ADPR-pDNA substrate to regenerate the pDNA strand | Aeropyrum pernix | ? | - |
- |
|
| additional information | enzyme ApeTpt1 catalyzes a one-step synthesis of a 5'-phospho-ADP-ribosylated cap structure (with no subsequent 5'-phosphotransferase step). ApeTpt1 caps RNA or DNA 5'-phosphate ends with ADP-ribose. pDNA and pRNA substrates are capped quantitatively at saturating enzyme in preence of NAD+. ApeTpt1 with 200 nM 10mer 5' 32P-labeled RNA or DNA oligonucleotides of identical nucleotide sequence (excepting T for U in DNA versus RNA) in the presence of 1 mM NAD+ resulted in the conversion of the substrates into RNA and DNA products of slower electrophoretic mobility. The 32P-labeled capped oligonucleotides are gel-purified and reacted with ApeTpt1 in the presence of 1 mM nicotinamide. This results in decapping of 20% of the ADPR-pDNA substrate to regenerate the pDNA strand | Aeropyrum pernix ATCC 700893 | ? | - |
- |
|
| additional information | enzyme ApeTpt1 catalyzes a one-step synthesis of a 5'-phospho-ADP-ribosylated cap structure (with no subsequent 5'-phosphotransferase step). ApeTpt1 caps RNA or DNA 5'-phosphate ends with ADP-ribose. pDNA and pRNA substrates are capped quantitatively at saturating enzyme in preence of NAD+. ApeTpt1 with 200 nM 10mer 5' 32P-labeled RNA or DNA oligonucleotides of identical nucleotide sequence (excepting T for U in DNA versus RNA) in the presence of 1 mM NAD+ resulted in the conversion of the substrates into RNA and DNA products of slower electrophoretic mobility. The 32P-labeled capped oligonucleotides are gel-purified and reacted with ApeTpt1 in the presence of 1 mM nicotinamide. This results in decapping of 20% of the ADPR-pDNA substrate to regenerate the pDNA strand | Aeropyrum pernix DSM 11879 | ? | - |
- |
|
| additional information | enzyme ApeTpt1 catalyzes a one-step synthesis of a 5'-phospho-ADP-ribosylated cap structure (with no subsequent 5'-phosphotransferase step). ApeTpt1 caps RNA or DNA 5'-phosphate ends with ADP-ribose. pDNA and pRNA substrates are capped quantitatively at saturating enzyme in preence of NAD+. ApeTpt1 with 200 nM 10mer 5' 32P-labeled RNA or DNA oligonucleotides of identical nucleotide sequence (excepting T for U in DNA versus RNA) in the presence of 1 mM NAD+ resulted in the conversion of the substrates into RNA and DNA products of slower electrophoretic mobility. The 32P-labeled capped oligonucleotides are gel-purified and reacted with ApeTpt1 in the presence of 1 mM nicotinamide. This results in decapping of 20% of the ADPR-pDNA substrate to regenerate the pDNA strand | Aeropyrum pernix JCM 9820 | ? | - |
- |
|
| additional information | enzyme ApeTpt1 catalyzes a one-step synthesis of a 5'-phospho-ADP-ribosylated cap structure (with no subsequent 5'-phosphotransferase step). ApeTpt1 caps RNA or DNA 5'-phosphate ends with ADP-ribose. pDNA and pRNA substrates are capped quantitatively at saturating enzyme in preence of NAD+. ApeTpt1 with 200 nM 10mer 5' 32P-labeled RNA or DNA oligonucleotides of identical nucleotide sequence (excepting T for U in DNA versus RNA) in the presence of 1 mM NAD+ resulted in the conversion of the substrates into RNA and DNA products of slower electrophoretic mobility. The 32P-labeled capped oligonucleotides are gel-purified and reacted with ApeTpt1 in the presence of 1 mM nicotinamide. This results in decapping of 20% of the ADPR-pDNA substrate to regenerate the pDNA strand | Aeropyrum pernix NBRC 100138 | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ApeTpt1 | - |
Aeropyrum pernix |
| CTHT_0025470 | - |
Thermochaetoides thermophila |
| KptA | - |
Acetivibrio thermocellus |
| KptA | - |
Aeropyrum pernix |
| KptA | - |
Pyrococcus horikoshii |
| kptA1 | - |
Archaeoglobus fulgidus |
| RNA 2'-phosphotransferase | - |
Homo sapiens |
| RNA 2'-phosphotransferase | - |
Saccharomyces cerevisiae |
| RNA 2'-phosphotransferase | - |
Runella slithyformis |
| RNA 2'-phosphotransferase | - |
Acetivibrio thermocellus |
| RNA 2'-phosphotransferase | - |
Thermochaetoides thermophila |
| RNA 2'-phosphotransferase | - |
Aeropyrum pernix |
| RNA 2'-phosphotransferase | - |
Pyrococcus horikoshii |
| RNA 2'-phosphotransferase | - |
Archaeoglobus fulgidus |
| RslTpt1 | - |
Runella slithyformis |
| Tpt1 | - |
Homo sapiens |
| Tpt1 | - |
Saccharomyces cerevisiae |
| Tpt1 | - |
Runella slithyformis |
| Tpt1 | - |
Acetivibrio thermocellus |
| Tpt1 | - |
Thermochaetoides thermophila |
| Tpt1 | - |
Aeropyrum pernix |
| Tpt1 | - |
Pyrococcus horikoshii |
| Tpt1 | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Saccharomyces cerevisiae |
| 37 | - |
assay at | Runella slithyformis |
| 37 | - |
assay at | Acetivibrio thermocellus |
| 37 | - |
assay at | Thermochaetoides thermophila |
| 37 | - |
assay at | Aeropyrum pernix |
| 37 | - |
assay at | Pyrococcus horikoshii |
| 37 | - |
assay at | Archaeoglobus fulgidus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 95 | ApeTpt1 caps the pDNA substrate with high yield (94-100%) at temperatures ranging from 37°C to 65°C and with progressively lower yields at 75°C (68%), 85°C (32%), and 95°C (21%) | Aeropyrum pernix |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
| 7.5 | - |
assay at | Saccharomyces cerevisiae |
| 7.5 | - |
assay at | Runella slithyformis |
| 7.5 | - |
assay at | Acetivibrio thermocellus |
| 7.5 | - |
assay at | Thermochaetoides thermophila |
| 7.5 | - |
assay at | Aeropyrum pernix |
| 7.5 | - |
assay at | Pyrococcus horikoshii |
| 7.5 | - |
assay at | Archaeoglobus fulgidus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Homo sapiens | |
| NAD+ | - |
Saccharomyces cerevisiae | |
| NAD+ | - |
Runella slithyformis | |
| NAD+ | - |
Acetivibrio thermocellus | |
| NAD+ | - |
Thermochaetoides thermophila | |
| NAD+ | - |
Aeropyrum pernix | |
| NAD+ | - |
Pyrococcus horikoshii | |
| NAD+ | - |
Archaeoglobus fulgidus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | wide distribution of Tpt1 enzymes in taxa that have no fungal-type RNA ligase. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-PO4 to a 2'-OH (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Homo sapiens |
| evolution | wide distribution of Tpt1 enzymes in taxa that have no fungal-type RNA ligase. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-PO4 to a 2'-OH (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Saccharomyces cerevisiae |
| evolution | wide distribution of Tpt1 enzymes in taxa that have no fungal-type RNA ligase. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-PO4 to a 2'-OH (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Runella slithyformis |
| evolution | wide distribution of Tpt1 enzymes in taxa that have no fungal-type RNA ligase. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-PO4 to a 2'-OH (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Acetivibrio thermocellus |
| evolution | wide distribution of Tpt1 enzymes in taxa that have no fungal-type RNA ligase. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-PO4 to a 2'-OH (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Thermochaetoides thermophila |
| evolution | wide distribution of Tpt1 enzymes in taxa that have no fungal-type RNA ligase. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-PO4 to a 2'-OH (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Pyrococcus horikoshii |
| evolution | wide distribution of Tpt1 enzymes in taxa that have no fungal-type RNA ligase. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-PO4 to a 2'-OH (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Archaeoglobus fulgidus |
| evolution | wide distribution of Tpt1 enzymes in taxa that have no fungal-type RNA ligase. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-PO4 to a 2'-OH (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus. Aeropyrum pernix Tpt1 (ApeTpt1) is particularly adept in this respect | Aeropyrum pernix |
| physiological function | the RNA 2'-phosphotransferase Tpt1 converts an internal RNA 2'-phosphate to a 2'-OH via a two-step NAD+-dependent mechanism in which: (i) the 2'-phosphate attacks the C1'' of NAD+ to expel nicotinamide and form a 2'-phospho-ADP-ribosylated RNA intermediate, and (ii) the ADP-ribose O2'' attacks the phosphate of the RNA 2'-phospho-ADPR intermediate to expel the RNA 2'-OH and generate ADP-ribose 1''-2'' cyclic phosphate. Tpt1 is an essential component of the fungal tRNA splicing pathway that generates a unique 2'-phosphate, 3'-5' phosphodiester splice junction during tRNA ligation. Tpt1 can catalyze reactions other than RNA 2'-phosphate removal. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-phosphate to a 2'-hydroxy (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus. Aeropyrum pernix Tpt1 (ApeTpt1) is particularly adept in this respect | Aeropyrum pernix |
| physiological function | the RNA 2'-phosphotransferase Tpt1 converts an internal RNA 2'-phosphate to a 2'-OH via a two-step NAD+-dependent mechanism in which: (i) the 2'-phosphate attacks the C1'' of NAD+ to expel nicotinamide and form a 2'-phospho-ADP-ribosylated RNA intermediate, and (ii) the ADP-ribose O2'' attacks the phosphate of the RNA 2'-phospho-ADPR intermediate to expel the RNA 2'-OH and generate ADP-ribose 1''-2'' cyclic phosphate. Tpt1 is an essential component of the fungal tRNA splicing pathway that generates a unique 2'-phosphate, 3'-5'-phosphodiester splice junction during tRNA ligation. Tpt1 can catalyze reactions other than RNA 2'-phosphate removal. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-phosphate to a 2'-hydroxy (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Homo sapiens |
| physiological function | the RNA 2'-phosphotransferase Tpt1 converts an internal RNA 2'-phosphate to a 2'-OH via a two-step NAD+-dependent mechanism in which: (i) the 2'-phosphate attacks the C1'' of NAD+ to expel nicotinamide and form a 2'-phospho-ADP-ribosylated RNA intermediate, and (ii) the ADP-ribose O2'' attacks the phosphate of the RNA 2'-phospho-ADPR intermediate to expel the RNA 2'-OH and generate ADP-ribose 1''-2'' cyclic phosphate. Tpt1 is an essential component of the fungal tRNA splicing pathway that generates a unique 2'-phosphate, 3'-5'-phosphodiester splice junction during tRNA ligation. Tpt1 can catalyze reactions other than RNA 2'-phosphate removal. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-phosphate to a 2'-hydroxy (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Saccharomyces cerevisiae |
| physiological function | the RNA 2'-phosphotransferase Tpt1 converts an internal RNA 2'-phosphate to a 2'-OH via a two-step NAD+-dependent mechanism in which: (i) the 2'-phosphate attacks the C1'' of NAD+ to expel nicotinamide and form a 2'-phospho-ADP-ribosylated RNA intermediate, and (ii) the ADP-ribose O2'' attacks the phosphate of the RNA 2'-phospho-ADPR intermediate to expel the RNA 2'-OH and generate ADP-ribose 1''-2'' cyclic phosphate. Tpt1 is an essential component of the fungal tRNA splicing pathway that generates a unique 2'-phosphate, 3'-5'-phosphodiester splice junction during tRNA ligation. Tpt1 can catalyze reactions other than RNA 2'-phosphate removal. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-phosphate to a 2'-hydroxy (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Runella slithyformis |
| physiological function | the RNA 2'-phosphotransferase Tpt1 converts an internal RNA 2'-phosphate to a 2'-OH via a two-step NAD+-dependent mechanism in which: (i) the 2'-phosphate attacks the C1'' of NAD+ to expel nicotinamide and form a 2'-phospho-ADP-ribosylated RNA intermediate, and (ii) the ADP-ribose O2'' attacks the phosphate of the RNA 2'-phospho-ADPR intermediate to expel the RNA 2'-OH and generate ADP-ribose 1''-2'' cyclic phosphate. Tpt1 is an essential component of the fungal tRNA splicing pathway that generates a unique 2'-phosphate, 3'-5'-phosphodiester splice junction during tRNA ligation. Tpt1 can catalyze reactions other than RNA 2'-phosphate removal. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-phosphate to a 2'-hydroxy (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Acetivibrio thermocellus |
| physiological function | the RNA 2'-phosphotransferase Tpt1 converts an internal RNA 2'-phosphate to a 2'-OH via a two-step NAD+-dependent mechanism in which: (i) the 2'-phosphate attacks the C1'' of NAD+ to expel nicotinamide and form a 2'-phospho-ADP-ribosylated RNA intermediate, and (ii) the ADP-ribose O2'' attacks the phosphate of the RNA 2'-phospho-ADPR intermediate to expel the RNA 2'-OH and generate ADP-ribose 1''-2'' cyclic phosphate. Tpt1 is an essential component of the fungal tRNA splicing pathway that generates a unique 2'-phosphate, 3'-5'-phosphodiester splice junction during tRNA ligation. Tpt1 can catalyze reactions other than RNA 2'-phosphate removal. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-phosphate to a 2'-hydroxy (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Thermochaetoides thermophila |
| physiological function | the RNA 2'-phosphotransferase Tpt1 converts an internal RNA 2'-phosphate to a 2'-OH via a two-step NAD+-dependent mechanism in which: (i) the 2'-phosphate attacks the C1'' of NAD+ to expel nicotinamide and form a 2'-phospho-ADP-ribosylated RNA intermediate, and (ii) the ADP-ribose O2'' attacks the phosphate of the RNA 2'-phospho-ADPR intermediate to expel the RNA 2'-OH and generate ADP-ribose 1''-2'' cyclic phosphate. Tpt1 is an essential component of the fungal tRNA splicing pathway that generates a unique 2'-phosphate, 3'-5'-phosphodiester splice junction during tRNA ligation. Tpt1 can catalyze reactions other than RNA 2'-phosphate removal. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-phosphate to a 2'-hydroxy (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Pyrococcus horikoshii |
| physiological function | the RNA 2'-phosphotransferase Tpt1 converts an internal RNA 2'-phosphate to a 2'-OH via a two-step NAD+-dependent mechanism in which: (i) the 2'-phosphate attacks the C1'' of NAD+ to expel nicotinamide and form a 2'-phospho-ADP-ribosylated RNA intermediate, and (ii) the ADP-ribose O2'' attacks the phosphate of the RNA 2'-phospho-ADPR intermediate to expel the RNA 2'-OH and generate ADP-ribose 1''-2'' cyclic phosphate. Tpt1 is an essential component of the fungal tRNA splicing pathway that generates a unique 2'-phosphate, 3'-5'-phosphodiester splice junction during tRNA ligation. Tpt1 can catalyze reactions other than RNA 2'-phosphate removal. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-phosphate to a 2'-hydroxy (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Archaeoglobus fulgidus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
