Cloned (Comment) | Organism |
---|---|
expression from Saccharomyces cerevisiae vectors | Saccharomyces cerevisiae |
expression in Saccharomyces cerevisiae | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F72A | temperature sensitive | Saccharomyces cerevisiae |
H117A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
H117A-T119A | lethal in vivo | Saccharomyces cerevisiae |
H142A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
H22A | lethal in vivo | Escherichia coli |
H24A | lethal in vivo | Saccharomyces cerevisiae |
H24N | lethal in vivo | Saccharomyces cerevisiae |
H24Q | lethal in vivo | Saccharomyces cerevisiae |
H90A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
K16A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
K69A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
R125A | lethal in vivo | Escherichia coli |
R138A | lethal in vivo | Saccharomyces cerevisiae |
R138K | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
R138Q | lethal in vivo | Saccharomyces cerevisiae |
R158A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
R21A | lethal in vivo | Escherichia coli |
R23A | lethal in vivo | Saccharomyces cerevisiae |
R23K | lethal in vivo | Saccharomyces cerevisiae |
R23Q | lethal in vivo | Saccharomyces cerevisiae |
R69A | lethal in vivo | Escherichia coli |
R71A | lethal in vivo | Saccharomyces cerevisiae |
R71K | lethal in vivo | Saccharomyces cerevisiae |
R71Q | grows slower than wild-type, temperature sensitive | Saccharomyces cerevisiae |
S91A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
T119A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
Y38A | colony size indistinguishable from that of wild-type TPT1 strain | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | - |
- |
- |
Archaeoglobus fulgidus | - |
- |
- |
Clostridium perfringens | - |
- |
- |
Drosophila melanogaster | - |
- |
- |
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Leishmania major | - |
- |
- |
Nostoc punctiforme | - |
- |
- |
Pyrococcus horikoshii | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Schizosaccharomyces pombe | - |
- |
- |
Trypanosoma cruzi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-agarose chromatography | Escherichia coli |
Ni-agarose chromatography | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-80, Tris-HCl buffer, pH 8.0, 10% glycerol | Saccharomyces cerevisiae |
Subunits | Comment | Organism |
---|---|---|
monomer | SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
KptA | - |
Escherichia coli |
Tpt1 | - |
Saccharomyces cerevisiae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
18 | 37 | growth of mutants K16A, K69A, H90A, S91A, H117A, T119A, H142A, R158A and R138K on yeast medium/peptone/dextrose agar, colony sizes were similar to that of wild-type TPT1 cells | Saccharomyces cerevisiae |
18 | 30 | F27A, R71Q, TPT1(1-220) and TPT1(1-210) cells failed to grow at 37°C on yeast medium/peptone/dextrose agar, R71Q and TPT1(1-210) strains grew slower than wild-type TPT1 | Saccharomyces cerevisiae |
25 | 30 | growth of mutants K16A, Y38A, K69A, F72A, H90A, S91A, H117A, T119A, H142A, R158A, TPT1(1-220) and TPT1(1-210) on 5-fluoroorotic acid agar medium | Saccharomyces cerevisiae |