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Literature summary for 2.7.1.16 extracted from

  • Zhang, L.; Leyn, S.A.; Gu, Y.; Jiang, W.; Rodionov, D.A.; Yang, C.
    Ribulokinase and transcriptional regulation of arabinose metabolism in Clostridium acetobutylicum (2012), J. Bacteriol., 194, 1055-1064.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Clostridium acetobutylicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.96
-
 L-ribulose in 50 mM Tris buffer (pH 7.5), at 30°C Clostridium acetobutylicum

Organism

Organism UniProt Comment Textmining
Clostridium acetobutylicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography Clostridium acetobutylicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-ribulose the enzyme is highly specific to L-ribulose Clostridium acetobutylicum ADP + L-ribulose 5-phosphate
-
 ?
additional information the enzyme is incapable of phosphorylating D-xylulose, D-glucose, D-fructose, and D-ribose Clostridium acetobutylicum ?
-
 ?

Synonyms

Synonyms Comment Organism
AraK
-
 Clostridium acetobutylicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
41
-
 L-ribulose in 50 mM Tris buffer (pH 7.5), at 30°C Clostridium acetobutylicum

General Information

General Information Comment Organism
malfunction inactivation of the araK gene severely impairs the growth on arabinose Clostridium acetobutylicum