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Literature summary for 2.7.1.159 extracted from

  • Marathe, A.; Krishnan, V.; Mahajan, M.M.; Thimmegowda, V.; Dahuja, A.; Jolly, M.; Praveen, S.; Sachdev, A.
    Characterization and molecular modeling of inositol 1,3,4 tris phosphate 5/6 kinase-2 from Glycine max (L) Merr. comprehending its evolutionary conservancy at functional level (2018), 3 Biotech, 8, 50 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.159

Cloned(Commentary)

Cloned (Comment) Organism
single copy Itpk2 gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged GmITPK2 in Escherichia coli strain BL21(DE3) Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic localization of GmITPK2 protein is predicted by CELLO tool with a reliability index of 2.976 Glycine max 5829
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 1,3,4-trisphosphate Glycine max
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 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
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 ?
ATP + 1D-myo-inositol 1,3,4-trisphosphate Glycine max
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 ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Glycine max A7X665 cv. Pusa-16, grown in the fields of Division of Genetics, I.A.R.I, New Delhi
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Source Tissue

Source Tissue Comment Organism Textmining
flower
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 Glycine max
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root
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 Glycine max
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seed spatio-temporal transcript profiling signified GmItpk2 to be seed-specific, with higher transcript levels in the early stage of seed development, GmItpk2 expression profiling overview Glycine max
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seedling
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 Glycine max
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stem
-
 Glycine max
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1,3,4-trisphosphate
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 Glycine max ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,3,4-trisphosphate
-
 Glycine max ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Glycine max
additional information the predicted three-dimensional model confirms 12 alpha-helices and 14 beta-barrel sheets with ATP-binding site close to beta-sheet present towards the C-terminus of the protein molecule. Secondary structure prediction of GmITPK2 using PDBSum tool Glycine max

Synonyms

Synonyms Comment Organism
GmItpk2
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 Glycine max
inositol 1,3,4 tris phosphate 5/6 kinase-2
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 Glycine max
inositol 1,3,4 trisphosphate kinase-2
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 Glycine max
inositol 1,4,5 trisphosphate 3/6 kinase
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 Glycine max
Ipk2
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 Glycine max
ITPK2
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 Glycine max

Cofactor

Cofactor Comment Organism Structure
ATP
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 Glycine max

pI Value

Organism Comment pI Value Maximum pI Value
Glycine max sequence calculation
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 5.9

Expression

Organism Comment Expression
Glycine max GmITPK2 is an early dehydration responsive gene which is also induced by dehydration and exogenous treatment with abscisic acid up

General Information

General Information Comment Organism
evolution phylogenetic tree showing the evolutionary relatedness of GmITPK2 with ITPKs of monocot and dicot species using Entamoeba histolytica (EhITPK) as an outgroup member Glycine max
metabolism following the formation of myo-inositol-3-phosphate, sequential phosphorylation reactions via action of various inositol phosphate kinases-myo-inositol kinase (Mik), inositol 1,3,4-trisphosphate kinase (Itpk), inositol 1,4,5-trisphosphate 3/6 kinase (Ipk2), and inositol 1,3,4,5,6-pentakisphosphate kinase (Ipk1) lead to phytic acid (PA, myo-inositol 1,2,3,4,5,6-hexakisphosphate) synthesis via the lipid-independent pathway and fine-tune the phosphorous flux towards PA accumulation Glycine max
additional information enzyme homology-based modeling and structure comparisons, overview. Active site mapping and molecular docking with ATP Glycine max
physiological function inositol 1,3,4-trisphosphate kinase-2 (GmItpk2), catalyzing the ATP-dependent phosphorylation of inositol 1,3,4-trisphosphate (IP3) to inositol 1,3,4,5-tetraphosphate or inositol 1,3,4,6-tetraphosphate, is a key enzyme diverting the flux of inositol phosphate pool towards phytate biosynthesis Glycine max