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Literature summary for 2.7.1.159 extracted from

  • Sun, Y.; Wilson, M.P.; Majerus, P.W.
    Inositol 1,3,4-trisphosphate 5/6-kinase associates with the COP9 signalosome by binding to CSN1 (2002), J. Biol. Chem., 277, 45759-45764.
    View publication on PubMed
Search for more literature for 2.7.1.159

Activating Compound

Activating Compound Comment Organism Structure
CSN1 the enzyme associates with the COP9 signalosome, an complex of 8 proteins, by binding to CSN1, activates Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of the enzyme tagged with 6 repeats of Myc at the N-terminus in Spodoptera frugiperda Sf21 cells using the baculovirus infection system, expression of His6-tagged and of FLAG-tagged enzyme in Escherichia coli strain BL21(DE3), co-overexpression of the enzyme with CSN1 in HeLa cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
curcumin inhibits both the free enzyme and the COP9 signalosome complex-associated kinase Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, recombinant enzyme with CSN1 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 COP9 signalosome, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from insect Sf21 cells to homogeneity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol-1,3,4-trisphosphate substrate of the free enzyme and the COP9 signalosome complex Homo sapiens ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol-1,3,4-trisphosphate substrate of the free enzyme and the COP9 signalosome complex Homo sapiens ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?
ATP + c-Jun substrate of the free enzyme and the COP9 signalosome complex, which both show no activity with the 1-79 residue fragment of c-Jun Homo sapiens ADP + phosphorylated c-Jun
-
 ?
ATP + IkappaBalpha substrate of the free enzyme and the COP9 signalosome complex Homo sapiens ADP + phosphorylated IkappaBalpha
-
 ?
ATP + p53 substrate of the free enzyme and the COP9 signalosome complex Homo sapiens ADP + phosphorylated p53
-
 ?
additional information the enzyme associates with the COP9 signalosome, an complex of 8 proteins, by binding to CSN1 Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 1D-myo-inositol-1,3,4-trisphosphate pH 7.6, 37°C, in absence of CSN1, first order rate constant Homo sapiens
20
-
 1D-myo-inositol-1,3,4-trisphosphate pH 7.6, 37°C, in presence of CSN1, first order rate constant Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens