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Literature summary for 2.7.1.159 extracted from
- Inositol 1,3,4-trisphosphate 5/6-kinase is a protein kinase that phosphorylates the transcription factors c-Jun and ATF-2 (2001), J. Biol. Chem., 276, 40998-41004.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in 293 cells, expression in Spodoptera frugiperda Sf21 cells using the baculovirus infection system | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1D-myo-inositol-3,4,5,6-tetrakisphosphate | inhibits the 5/6-kinase activities, but not the phosphorylation of ATF-2 | Bos taurus |  |
| Mn2+ | activates the protein phopshorylation, but inhibits the inositol-1,3,4-trisphosphate 5/6-kinase reaction | Bos taurus | |
| protein kinase A | slight inhibition of enzyme activity with ATF-2 as substrate | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Bos taurus | |
| Mg2+ | - |
Homo sapiens | |
| Mn2+ | activates the protein phopshorylation, but inhibits the inositol-1,3,4-trisphosphate 5/6-kinase reaction | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | Bos taurus | - |
ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | Bos taurus | - |
ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate | - |
? | |
| additional information | Bos taurus | the enzyme is involved in both the inositol phosphate and the stress response pathways which are linked by the enzyme, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially as COP9 signalosome complex and further as a single protein | Bos taurus |
| recombinant enzyme from insect Sf21 cells, recombinant enzyme partially from 293 cells by ion exchange chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 372 | - |
purified calf brain enzyme, substrate 1D-myo-inositol-1,3,4-trisphosphate | Bos taurus |
| 500 | - |
purified recombinant enzyme from Sf21 cells, with substrate 1D-myo-inositol-1,3,4-trisphosphate | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | - |
Bos taurus | ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | 5fold lower activity compared to 1D-myo-inositol-1,3,4,6-tetrakisphosphate formation | Bos taurus | ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | 5fold lower activity compared to 1D-myo-inositol-1,3,4,6-tetrakisphosphate formation | Homo sapiens | ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | - |
Bos taurus | ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | 5fold higher activity compared to 1D-myo-inositol-1,3,4,5-tetrakisphosphate formation | Bos taurus | ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | 5fold higher activity compared to 1D-myo-inositol-1,3,4,5-tetrakisphosphate formation | Homo sapiens | ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate | - |
? | |
| ATP + ATF-2 | phosphorylation predominantly on serine residues | Bos taurus | ADP + phosphorylated ATF-2 | - |
? | |
| ATP + ATF-2 | phosphorylation predominantly on serine residues | Homo sapiens | ADP + phosphorylated ATF-2 | - |
? | |
| ATP + c-Jun | - |
Bos taurus | ADP + phosphorylated c-Jun | - |
? | |
| ATP + c-Jun | - |
Homo sapiens | ADP + phosphorylated c-Jun | - |
? | |
| additional information | the enzyme is involved in both the inositol phosphate and the stress response pathways which are linked by the enzyme, overview | Bos taurus | ? | - |
? | |
| additional information | the enzyme is part of the COP9 signalosome complex consisting of 8 proteins | Bos taurus | ? | - |
? | |
| additional information | the enzyme is part of the COP9 signalosome complex consisting of 8 proteins | Homo sapiens | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bos taurus |
| 7.5 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bos taurus | |
| ATP | - |
Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00003 | - |
1D-myo-inositol-3,4,5,6-tetrakisphosphate | pH 7.5, 37°C | Bos taurus | |
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