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Literature summary for 2.7.1.159 extracted from

  • Abdullah, M.; Hughes, P.J.; Craxton, A.; Gigg, R.; Desai, T.; Marecek, J.F.; Prestwich, G.D.; Shears, S.B.
    Purification and characterization of inositol-1,3,4-trisphosphate 5/6-kinase from rat liver using an inositol hexakisphosphate affinity column (1992), J. Biol. Chem., 267, 22340-22345.
    View publication on PubMed
Search for more literature for 2.7.1.159

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme activity is unaffected by protein kinase C, protein kinase A, and Ca2+/calmodulin Rattus norvegicus

General Stability

General Stability Organism
addition of 1 mM ATP at pH 6.1 stabilizes the enzyme during purification Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
1D-myo-inositol-1,3,4,5-tetrakisphosphate competitive Rattus norvegicus
1D-myo-inositol-1,3,4,6-tetrakisphosphate competitive Rattus norvegicus
additional information enzyme activity is unaffected by protein kinase C, protein kinase A, and Ca2+/calmodulin Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 x * 36000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
26171fold from liver by polyethylene glycol precipitation, ion exchange chromatography and heparin and inositol hexakisphosphate affinity chromatograpies Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1780
-
 purified enzyme, determination of 5-kinase and 6-kinase activities Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol-1,3,4-trisphosphate 5fold lower activity compared to 1D-myo-inositol-1,3,4,6-tetrakisphosphate formation Rattus norvegicus ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol-1,3,4-trisphosphate 5fold higher activity compared to 1D-myo-inositol-1,3,4,5-tetrakisphosphate formation Rattus norvegicus ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
inositol-1,3,4-trisphosphate kinase
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.002 0.003 1D-myo-inositol-1,3,4,6-tetrakisphosphate pH 7.2, 37°C, versus 1D-myo-inositol-1,3,4-trisphosphate Rattus norvegicus
0.003 0.004 1D-myo-inositol-1,3,4,5-tetrakisphosphate pH 7.2, 37°C, versus 1D-myo-inositol-1,3,4-trisphosphate Rattus norvegicus