Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.159 extracted from

  • Wilson, M.P.; Majerus, P.W.
    Isolation of inositol 1,3,4-trisphosphate 5/6-kinase, cDNA cloning, and expression of the recombinant enzyme (1996), J. Biol. Chem., 271, 11904-11910.
    View publication on PubMed
Search for more literature for 2.7.1.159

Cloned(Commentary)

Cloned (Comment) Organism
DNA and N-terminal amino acid sequence determination and analysis Bos taurus
DNA and N-terminal amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00008
-
 1D-myo-inositol-1,3,4-trisphosphate
-
 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Bos taurus
Mg2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45600
-
 x * 45600, about, sequence calculation Homo sapiens
48000
-
 x * 36000-38000, proteolytically C-terminally truncated enzyme, SDS-PAGE, x * 48000, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-
Homo sapiens Q13572
-
-

Purification (Commentary)

Purification (Comment) Organism
12900fold from brain by ion exchange chromatography, and heparin/inositol hexakisphosphate affinity chromatography Bos taurus
recombinant His-tagged enzyme from Escherichia coli by adsorption and calmodulin affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Bos taurus
-
brain
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25 48 calf brain Bos taurus
372
-
 purified enzyme Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol-1,3,4-trisphosphate lower activity compared to 1D-myo-inositol-1,3,4,6-tetrakisphosphate formation Bos taurus ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol-1,3,4-trisphosphate lower activity compared to 1D-myo-inositol-1,3,4,6-tetrakisphosphate formation Homo sapiens ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol-1,3,4-trisphosphate higher activity compared to 1D-myo-inositol-1,3,4,5-tetrakisphosphate formation Bos taurus ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol-1,3,4-trisphosphate higher activity compared to 1D-myo-inositol-1,3,4,5-tetrakisphosphate formation Homo sapiens ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 36000-38000, proteolytically C-terminally truncated enzyme, SDS-PAGE, x * 48000, SDS-PAGE Bos taurus
? x * 45600, about, sequence calculation Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Bos taurus
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Bos taurus
ATP
-
 Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens sequence calculation
-
 6