Literature summary for 2.7.1.158 extracted from

Franco-Echevarria, E.; Sanz-Aparicio, J.; Troffer-Charlier, N.; Poterszman, A.; Gonzalez, B.
Crystallization and preliminary X-ray diffraction analysis of a mammal inositol 1,3,4,5,6-pentakisphosphate 2-kinase (2017), Protein J., 36, 240-248 .

Search for more literature for 2.7.1.158

Cloned(Commentary)

Cloned (Comment)	Organism
gene ipk1, recombinant expression of LSLt-tagged enzyme in Escherichia coli strain BL21 Star (DE3), and recombinant expression of GST-tagged enzyme in Spodoptera frugiperda Sf21 cells. LSLt is a fragment of Laetiporus sulphurous lectin	Mus musculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant detagged enzyme, sitting drop vapour diffusion, mixing of 250 nl of 5-6 mg/ml protein solution with 250 nl of crystallization solution containing 0.2 M MgCl2, 0.1 M MES, pH 6.25, and 18% v/v PEG 6000 for insect cell-expressed enzyme, and 0.2 M MgCl2, 0.1 M MES, pH 6.25, and 12% v/v PEG 6000 for bacteria-expressed enzyme, equilibration against 0.065 ml of crystallization solution as reservoir, method screening and optimization, X-ray diffraction structure determination and analysis at resolution of 4.3 A (Escherichia coli expression) and 4.0 A (insect cell expression), modeling	Mus musculus

Crystallization (Comment)

Organism

purified recombinant detagged enzyme, sitting drop vapour diffusion, mixing of 250 nl of 5-6 mg/ml protein solution with 250 nl of crystallization solution containing 0.2 M MgCl2, 0.1 M MES, pH 6.25, and 18% v/v PEG 6000 for insect cell-expressed enzyme, and 0.2 M MgCl2, 0.1 M MES, pH 6.25, and 12% v/v PEG 6000 for bacteria-expressed enzyme, equilibration against 0.065 ml of crystallization solution as reservoir, method screening and optimization, X-ray diffraction structure determination and analysis at resolution of 4.3 A (Escherichia coli expression) and 4.0 A (insect cell expression), modeling

Mus musculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate	Mus musculus	-	ADP + 1D-myo-inositol hexakisphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q6P1C1	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant LSLt-tagged enzyme from Escherichia coli strain BL21 Star (DE3) by heparin affinity chromatography and gel filtration followed by tag cleavage with TEV protease and again heparin affinity chromatography and gel filtration. The recombinant GST-tagged enzyme from Sf21 insect cells is purified by glutathione affinity chromatography and tag cleavage with PreScission protease, followed by heparin affinity chromatography and gel filtration	Mus musculus

Purification (Comment)

Organism

recombinant LSLt-tagged enzyme from Escherichia coli strain BL21 Star (DE3) by heparin affinity chromatography and gel filtration followed by tag cleavage with TEV protease and again heparin affinity chromatography and gel filtration. The recombinant GST-tagged enzyme from Sf21 insect cells is purified by glutathione affinity chromatography and tag cleavage with PreScission protease, followed by heparin affinity chromatography and gel filtration

Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate	-	Mus musculus	ADP + 1D-myo-inositol hexakisphosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	-	Mus musculus

Synonyms

Synonyms	Comment	Organism
inositol 1,3,4,5,6-pentakisphosphate 2-kinase	-	Mus musculus
IP5 2-K	-	Mus musculus
Ipk1	-	Mus musculus
mIP5 2-K	-	Mus musculus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Mus musculus

General Information

General Information	Comment	Organism
physiological function	inositol 1,3,4,5,6-pentakisphosphate 2-kinase (IP5 2-K) phosphorylates inositol pentakisphosphate (IP5) to produce inositol 1,2,3,4,5,6-hexakisphosphate (IP6), from ATP	Mus musculus