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Literature summary for 2.7.1.157 extracted from

  • Kristiansson, H.; Timson, D.J.
    N-acetylgalactosamine kinase: a naturally promiscuous small molecule kinase (2012), Appl. Biochem. Biotechnol., 166, 57-63.
    View publication on PubMed
Search for more literature for 2.7.1.157

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
F444A mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme Homo sapiens
F444C mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme but increases turnover rate with N-acetylgalactosamine Homo sapiens
F444H mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme Homo sapiens
F444K mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme Homo sapiens
F444R mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme Homo sapiens
F444S mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme but increases turnover rate with N-acetylgalactosamine Homo sapiens
F444W mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 ATP mutant F444A, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine Homo sapiens
0.009
-
 ATP mutant F444R, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine Homo sapiens
0.009
-
 ATP wild-type, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine Homo sapiens
0.011
-
 ATP mutant F444H, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine Homo sapiens
0.013
-
 ATP mutant F444H, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine Homo sapiens
0.013
-
 ATP mutant F444S, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine Homo sapiens
0.015
-
 ATP mutant F444K, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine Homo sapiens
0.015
-
 ATP mutant F444K, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine Homo sapiens
0.015
-
 ATP mutant F444R, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine Homo sapiens
0.017
-
 ATP mutant F444C, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine Homo sapiens
0.017
-
 ATP mutant F444H, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine Homo sapiens
0.018
-
 ATP mutant F444C, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine Homo sapiens
0.018
-
 ATP wild-type, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine Homo sapiens
0.019
-
 ATP mutant F444S, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine Homo sapiens
0.024
-
 ATP mutant F444R, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine Homo sapiens
0.024
-
 ATP mutant F444W, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine Homo sapiens
0.025
-
 ATP mutant F444A, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine Homo sapiens
0.027
-
 ATP mutant F444C, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine Homo sapiens
0.028
-
 ATP mutant F444K, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine Homo sapiens
0.032
-
 ATP wild-type, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine Homo sapiens
0.039
-
 ATP mutant F444A, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine Homo sapiens
0.051
-
 N-acetyl-alpha-D-glucosamine wild-type, 37°C, pH 7.5 Homo sapiens
0.12
-
 N-acetyl-alpha-D-glucosamine mutant F444K, 37°C, pH 7.5 Homo sapiens
0.12
-
 N-acetyl-alpha-D-glucosamine mutant F444S, 37°C, pH 7.5 Homo sapiens
0.13
-
 ATP mutant F444S, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine Homo sapiens
0.17
-
 N-acetyl-alpha-D-galactosamine mutant F444R, 37°C, pH 7.5 Homo sapiens
0.22
-
 N-acetyl-alpha-D-glucosamine mutant F444H, 37°C, pH 7.5 Homo sapiens
0.24
-
 N-acetyl-alpha-D-galactosamine mutant F444A, 37°C, pH 7.5 Homo sapiens
0.25
-
 N-acetyl-alpha-D-mannosamine mutant F444H, 37°C, pH 7.5 Homo sapiens
0.27
-
 N-acetyl-alpha-D-galactosamine mutant F444H, 37°C, pH 7.5 Homo sapiens
0.28
-
 N-acetyl-alpha-D-glucosamine mutant F444R, 37°C, pH 7.5 Homo sapiens
0.33
-
 N-acetyl-alpha-D-galactosamine wild-type, 37°C, pH 7.5 Homo sapiens
0.34
-
 N-acetyl-alpha-D-galactosamine mutant F444K, 37°C, pH 7.5 Homo sapiens
0.37
-
 N-acetyl-alpha-D-glucosamine mutant F444A, 37°C, pH 7.5 Homo sapiens
0.39
-
 N-acetyl-alpha-D-glucosamine mutant F444C, 37°C, pH 7.5 Homo sapiens
0.47
-
 N-acetyl-alpha-D-mannosamine mutant F444C, 37°C, pH 7.5 Homo sapiens
0.56
-
 N-acetyl-alpha-D-galactosamine mutant F444S, 37°C, pH 7.5 Homo sapiens
0.65
-
 N-acetyl-alpha-D-mannosamine mutant F444R, 37°C, pH 7.5 Homo sapiens
0.69
-
 N-acetyl-alpha-D-galactosamine mutant F444W, 37°C, pH 7.5 Homo sapiens
0.79
-
 N-acetyl-alpha-D-mannosamine wild-type, 37°C, pH 7.5 Homo sapiens
0.93
-
 N-acetyl-alpha-D-mannosamine mutant F444A, 37°C, pH 7.5 Homo sapiens
0.95
-
 N-acetyl-alpha-D-galactosamine mutant F444C, 37°C, pH 7.5 Homo sapiens
0.95
-
 N-acetyl-alpha-D-mannosamine mutant F444K, 37°C, pH 7.5 Homo sapiens
4.1
-
 N-acetyl-alpha-D-mannosamine mutant F444S, 37°C, pH 7.5 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q01415 isoform GALK2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-alpha-D-galactosamine
-
 Homo sapiens ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
ATP + N-acetyl-alpha-D-glucosamine
-
 Homo sapiens ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
ATP + N-acetyl-alpha-D-mannosamine
-
 Homo sapiens ADP + N-acetyl-alpha-D-mannosamine 1-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
GALK2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.053
-
 N-acetyl-alpha-D-mannosamine mutant F444H, 37°C, pH 7.5 Homo sapiens
0.063
-
 N-acetyl-alpha-D-glucosamine mutant F444S, 37°C, pH 7.5 Homo sapiens
0.066
-
 N-acetyl-alpha-D-mannosamine mutant F444C, 37°C, pH 7.5 Homo sapiens
0.071
-
 N-acetyl-alpha-D-glucosamine mutant F444R, 37°C, pH 7.5 Homo sapiens
0.072
-
 N-acetyl-alpha-D-glucosamine mutant F444H, 37°C, pH 7.5 Homo sapiens
0.087
-
 N-acetyl-alpha-D-glucosamine mutant F444C, 37°C, pH 7.5 Homo sapiens
0.11
-
 N-acetyl-alpha-D-glucosamine mutant F444A, 37°C, pH 7.5 Homo sapiens
0.16
-
 N-acetyl-alpha-D-glucosamine wild-type, 37°C, pH 7.5 Homo sapiens
0.18
-
 N-acetyl-alpha-D-glucosamine mutant F444K, 37°C, pH 7.5 Homo sapiens
0.19
-
 N-acetyl-alpha-D-mannosamine mutant F444R, 37°C, pH 7.5 Homo sapiens
0.19
-
 N-acetyl-alpha-D-mannosamine wild-type, 37°C, pH 7.5 Homo sapiens
0.23
-
 N-acetyl-alpha-D-mannosamine mutant F444A, 37°C, pH 7.5 Homo sapiens
0.25
-
 N-acetyl-alpha-D-mannosamine mutant F444K, 37°C, pH 7.5 Homo sapiens
0.4
-
 N-acetyl-alpha-D-mannosamine mutant F444S, 37°C, pH 7.5 Homo sapiens
0.62
-
 N-acetyl-alpha-D-galactosamine mutant F444W, 37°C, pH 7.5 Homo sapiens
0.72
-
 N-acetyl-alpha-D-galactosamine mutant F444R, 37°C, pH 7.5 Homo sapiens
1
-
 N-acetyl-alpha-D-galactosamine mutant F444K, 37°C, pH 7.5 Homo sapiens
1.2
-
 N-acetyl-alpha-D-galactosamine wild-type, 37°C, pH 7.5 Homo sapiens
1.6
-
 N-acetyl-alpha-D-galactosamine mutant F444H, 37°C, pH 7.5 Homo sapiens
1.9
-
 N-acetyl-alpha-D-galactosamine mutant F444A, 37°C, pH 7.5 Homo sapiens
2.6
-
 N-acetyl-alpha-D-galactosamine mutant F444C, 37°C, pH 7.5 Homo sapiens
3.2
-
 N-acetyl-alpha-D-galactosamine mutant F444S, 37°C, pH 7.5 Homo sapiens