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Literature summary for 2.7.1.157 extracted from
- Mechanistic studies on human N-acetylgalactosamine kinase (2009), J. Enzyme Inhib. Med. Chem., 25, 370-376.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Homo sapiens |
| GALK2, functional expression in Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-methylgalactopyranoside | uncompetitive inhibition with respect to ATP; uncompetitive with respect to ATP, poor inhibitor | Homo sapiens |  |
| N-acetyl-D-galactosamine | substrate inhibition | Homo sapiens | |
| N-acetylgalactosamine | substrate inhibition | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
ATP | recombinant enzyme | Homo sapiens | |
| 0.014 | - |
ATP | pH 8.0, 37°C | Homo sapiens | |
| 0.04 | - |
N-acetyl-D-galactosamine | recombinant enzyme | Homo sapiens | |
| 0.04 | - |
N-acetyl-alpha-D-galactosamine | pH 8.0, 37°C | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + N-acetyl-D-galactosamine | Homo sapiens | - |
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Homo sapiens | Q01415 | isoform GALK2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GALK2 from Escherichia coli | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + N-acetyl-alpha-D-galactosamine = ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | ordered ternary complex reaction mechanism, in which ATP binds first, overview. Ionizable residues play a role in substrate binding and catalysis | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + N-acetyl-alpha-D-galactosamine | - |
Homo sapiens | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
| ATP + N-acetyl-D-galactosamine | - |
Homo sapiens | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
| additional information | ordered ternary complex mechanism in which ATP is the first substrate to bind | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GALK2 | - |
Homo sapiens |
| More | GALK2 belongs to the GHMP family of enzymes | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
ATP | recombinant enzyme | Homo sapiens | |
| 1 | - |
N-acetyl-D-galactosamine | recombinant enzyme | Homo sapiens | |
| 1 | - |
N-acetyl-alpha-D-galactosamine | pH 8.0, 37°C | Homo sapiens | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH dependency of the reaction mechanism, overview | Homo sapiens |
| 7 | 8 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13 | - |
N-acetylgalactosamine | pH 8.0, 37°C | Homo sapiens | |
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