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Literature summary for 2.7.1.157 extracted from

  • Pastuszak, I.; Drake, R.; Elbein, A.D.
    Kidney N-acetylgalactosamine (GalNAc)-1-phosphate kinase, a new pathway of GalNAc activation (1996), J. Biol. Chem., 271, 20776-20782.
    View publication on PubMed
Search for more literature for 2.7.1.157

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
 ATP
-
 Sus scrofa
0.14
-
 N-acetyl-D-galactosamine
-
 Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Sus scrofa 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation required, efficiency in descending order: Mg2+ > Mn2+ > Co2+, optimum Co2+ concentration is 10 mM, maximal activity is 50% of that with Mg2+ Sus scrofa
Mg2+ divalent cation required, efficiency in descending order: Mg2+ > Mn2+ > Co2+, optimum Mg2+ concentration is 5 mM Sus scrofa
Mn2+ divalent cation required, efficiency in descending order: Mg2+ > Mn2+ > Co2+, optimum Mn2+ concentration is 2-8 mM, maximal activity is 50% of that with Mg2+ Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Oryctolagus cuniculus
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Homo sapiens
-
kidney
-
 Oryctolagus cuniculus
-
kidney highest total and specific activity Sus scrofa
-
liver
-
 Sus scrofa
-
liver
-
 Oryctolagus cuniculus
-
lung
-
 Sus scrofa
-
additional information no activity in pancreas, brain, aorta, heart Sus scrofa
-
spleen
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.43
-
-
 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-phosphate + N-acetyl-alpha-D-galactosamine 8% of the specific activity with ATP Sus scrofa acetate + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
ATP + N-acetyl-alpha-D-galactosamine
-
 Homo sapiens ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
ATP + N-acetyl-alpha-D-galactosamine
-
 Sus scrofa ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
ATP + N-acetyl-alpha-D-galactosamine
-
 Oryctolagus cuniculus ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
ATP + N-acetyl-D-galactosamine
-
 Sus scrofa ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
ITP + N-acetyl-alpha-D-galactosamine 9% of the specific activity with ATP Sus scrofa IDP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
phosphoenolpyruvate + N-acetyl-alpha-D-galactosamine 11% of the specific activity with ATP Sus scrofa pyruvate + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Sus scrofa

Synonyms

Synonyms Comment Organism
GALK2
-
 Homo sapiens
GALK2
-
 Sus scrofa
GALK2
-
 Oryctolagus cuniculus
GalNAc kinase
-
 Homo sapiens
GalNAc kinase
-
 Sus scrofa
GalNAc kinase
-
 Oryctolagus cuniculus
GK2
-
 Homo sapiens
GK2
-
 Sus scrofa
GK2
-
 Oryctolagus cuniculus
N-acetylgalactosamine (GalNAc)-1-phosphate kinase
-
 Homo sapiens
N-acetylgalactosamine (GalNAc)-1-phosphate kinase
-
 Sus scrofa
N-acetylgalactosamine (GalNAc)-1-phosphate kinase
-
 Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6.0: about 45% of maximal activity, pH 10.0: about 35% of maximal activity Sus scrofa