Cloned (Comment) | Organism |
---|---|
Chromosomal in-frame deletion mutations are constructed for the cobU gene using linear transformation and moved into strains with the deletion mutation apt-18, which causes strains to synthesize only pseudo-B12 cofactors. | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | The cobU mutations all eliminate the ability of the apt-18 deletion mutant to grow on cobinamide | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosylcobinamide phosphate + GTP | Salmonella enterica subsp. enterica serovar Typhimurium | - |
adenosylcobinamide-GDP + diphosphate | - |
? | |
additional information | Salmonella enterica subsp. enterica serovar Typhimurium | CobU enzyme is required for synthesis of both coenzyme B12 (alpha ligand: 5,6-dimethylbenzimidazole) and pseudo-coenzyme B12 (alpha ligand: adenine). | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
RTP + adenosylcobinamide = adenosylcobinamide phosphate + RDP | Aminopropanol side chain of the corrin cobinamide is activated by the addition of GDP. Synthesis of the coenzyme pseudo-B12 cofactors requires the same enzymes (e.g. CobU) which install 5,6-dimethylbenzimidazole in the formation of coenzyme B12 | Salmonella enterica subsp. enterica serovar Typhimurium |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | strain LT2 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosylcobinamide phosphate + GTP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobinamide-GDP + diphosphate | - |
? | |
additional information | CobU enzyme is required for synthesis of both coenzyme B12 (alpha ligand: 5,6-dimethylbenzimidazole) and pseudo-coenzyme B12 (alpha ligand: adenine). | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CobU | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
GTP | - |
Salmonella enterica subsp. enterica serovar Typhimurium |