Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.156 extracted from

  • Thomas, M.G.; Thompson, T.B.; Rayment, I.; Escalante-Semerena, J.C.
    Analysis of the adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU) enzyme of Salmonella typhimurium LT2. Identification of residue His-46 as the site of guanylylation (2000), J. Biol. Chem., 275, 27576-27586.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
H45A site-directed mutagenesis Salmonella enterica subsp. enterica serovar Typhimurium
H45A/H46A site-directed mutagenesis Salmonella enterica subsp. enterica serovar Typhimurium
H46A site-directed mutagenesis Salmonella enterica subsp. enterica serovar Typhimurium
H46N site-directed mutagenesis Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
adenosylcobinamide-phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium
diphosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + adenosylcobinamide Salmonella enterica subsp. enterica serovar Typhimurium
-
adenosylcobinamide phosphate + ADP
-
?
ATP + adenosylcobinamide Salmonella enterica subsp. enterica serovar Typhimurium late steps of cobalamin biosynthesis, involved in de novo synthesis of vitamin B12, nucleotide loop assembly, enzyme functions only if Salmonella typhimurium is assimilating cobinamide from its environment, CobU becomes less active during the kinase reaction due to the formation of a covalent CobU-AMP complex that holds CobU in an altered conformation adenosylcobinamide phosphate + ADP
-
?
GTP + adenosylcobinamide Salmonella enterica subsp. enterica serovar Typhimurium
-
adenosylcobinamide phosphate + GDP
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00000003
-
GTP:kinase specific activity, mutant H46A Salmonella enterica subsp. enterica serovar Typhimurium
0.00000008
-
GTP:kinase specific activity, mutant H46N Salmonella enterica subsp. enterica serovar Typhimurium
0.000251
-
GTP:kinase specific activity, mutant H45A Salmonella enterica subsp. enterica serovar Typhimurium
0.001166
-
GTP:kinase specific activity, wild-type Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-deoxyadenosylcobinamide highest kinase activity, product generation relative to adenosylcobinamide 102% Salmonella enterica subsp. enterica serovar Typhimurium 5'-deoxyadenosylcobinamide phosphate + ADP
-
?
ATP + 5'-deoxycytosylcobinamide about 65% of the activity with AdoCbi Salmonella enterica subsp. enterica serovar Typhimurium 5'-deoxycytosylcobinamide phosphate + ADP
-
?
ATP + 5'-deoxyinosylcobinamide about 55% of the activity with AdoCbi Salmonella enterica subsp. enterica serovar Typhimurium 5'-deoxyinosylcobinamide phosphate + ADP
-
?
ATP + adenosylcobinamide
-
Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + ADP
-
?
ATP + adenosylcobinamide late steps of cobalamin biosynthesis, involved in de novo synthesis of vitamin B12, nucleotide loop assembly, enzyme functions only if Salmonella typhimurium is assimilating cobinamide from its environment, CobU becomes less active during the kinase reaction due to the formation of a covalent CobU-AMP complex that holds CobU in an altered conformation Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + ADP
-
?
dATP + adenosylcobinamide
-
Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + dADP
-
?
dGTP + adenosylcobinamide
-
Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + dGDP
-
?
GTP + adenosylcobinamide
-
Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + GDP
-
?
additional information (CN)2Cbi is no substrate, no product formation with CTP, UTP or dCTP Salmonella enterica subsp. enterica serovar Typhimurium ?
-
?

Synonyms

Synonyms Comment Organism
adenosylcobinamide kinase
-
Salmonella enterica subsp. enterica serovar Typhimurium
CobU
-
Salmonella enterica subsp. enterica serovar Typhimurium
CobU AdoCbi kinase
-
Salmonella enterica subsp. enterica serovar Typhimurium