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Literature summary for 2.7.1.154 extracted from

  • Crljen, V.; Volinia, S.; Banfic, H.
    Hepatocyte growth factor activates phosphoinositide 3-kinase C2beta in renal brush-border plasma membranes (2002), Biochem. J., 365, 791-799.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.154

Activating Compound

Activating Compound Comment Organism Structure
A23187 Ca2+-ionophore, activates enzyme form PI3K-C2beta, mimicks hepatocyte growth factor HGF Rattus norvegicus
hepatocyte growth factor HGF in renal brush-border and basal lateral plasma membranes, in the latter case mediated by calpain Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
1,2-bis-(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid i.e. BAPTA-AM, a cell-penetrating Ca2+-chelator Rattus norvegicus
calpeptin inhibits proteolytic activation of enzyme form PI3K C2beta in brush border plasma membranes Rattus norvegicus
Wortmannin phosphoinositide 3-kinase C2beta Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane renal brush-border and basal lateral, enzyme form phosphoinositide 3-kinase C2beta occurs only in renal brush-border plasma membrane Rattus norvegicus 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Rattus norvegicus
Mg2+
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
 x * 180000, enzyme form PI3K C2beta, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate Rattus norvegicus
-
 ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 class II enzyme
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification activation of enzyme form PI3K C2beta occurs probably via proteolysis in the brush broder plasma membrane Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
partially by subcellular fractionation of renal cortex, plasma membrane preparation Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Rattus norvegicus
-
renal cortex
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol preferred substrate of enzyme form PI3K-C2beta Rattus norvegicus ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Rattus norvegicus ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 180000, enzyme form PI3K C2beta, SDS-PAGE Rattus norvegicus
More class II PI3K enzymes are distinguished from other PI3K isoenzymes by the presence of two tandem domains at their C-termini, the first one is termed a Phox homology domain, which is revealed as a novel phosphoinositide-binding domain, whereas the second is the C2 domain, which is a phospholipid-binding domain that can confer Ca2+ sensitivity Rattus norvegicus

Synonyms

Synonyms Comment Organism
phosphoinositide 3-kinase
-
 Rattus norvegicus
phosphoinositide 3-kinase C2beta
-
 Rattus norvegicus
PI3K
-
 Rattus norvegicus