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Literature summary for 2.7.1.154 extracted from
- Cloning of a human phosphoinositide 3-kinase with a C2 domain that displays reduced sensitivity to the inhibitor wortmannin (1997), Biochem. J., 326, 139-147.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| LY294002 | IC50: 0.019 mM, maximal inhibition at 1 mM | Homo sapiens |  |
| additional information | introduction of phosphatidylserine, produces an 8fold decrease in Vmax for ATP using phosphatidylinositol. Without phosphatidylserine, phosphatidylinositol 4-phosphate produces non-linear enzyme kinetics | Homo sapiens | |
| Wortmannin | IC50: 420 nM, maximal inhibition at 10 mM | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
ATP | pH 7.5, 30°C, reaction with phosphatidylinositol, without phosphatidylserine | Homo sapiens | |
| 0.025 | - |
phosphatidylinositol 4-phosphate | pH 7.5, 30°C, in presence of phosphatidylserine | Homo sapiens | |
| 0.032 | - |
ATP | pH 7.5, 30°C, reaction with phosphatidylinositol, in presence of phosphatidylserine | Homo sapiens | |
| 0.054 | - |
ATP | pH 7.5, 30°C, reaction with phosphatidylinositol 4-phosphate, without phosphatidylserine | Homo sapiens | |
| 0.064 | - |
phosphatidylinositol | pH 7.5, 30°C, in presence of phosphatidylserine | Homo sapiens | |
| 0.122 | - |
phosphatidylinositol | pH 7.5, 30°C, without phosphatidylserine | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 190000 | - |
x * 190000, calculation from nucleotide sequence | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
PI3K-C2alpha | - |
| Homo sapiens PI3K-C2alpha | - |
PI3K-C2alpha | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Homo sapiens | - |
| kidney | mRNA is undetectable in kidney | Homo sapiens | - |
| ovary | - |
Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
| testis | - |
Homo sapiens | - |
| U-937 cell | monocyte | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-phosphatidyl-1D-myo-inositol-4-phosphate | - |
Homo sapiens | ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate | - |
? | |
| ATP + 1-phosphatidyl-1D-myo-inositol-4-phosphate | - |
Homo sapiens PI3K-C2alpha | ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate | - |
? | |
| ATP + phosphatidylinositol | - |
Homo sapiens | ADP + phosphatidylinositol 3-phosphate | - |
? | |
| ATP + phosphatidylinositol | - |
Homo sapiens PI3K-C2alpha | ADP + phosphatidylinositol 3-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 190000, calculation from nucleotide sequence | Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00042 | - |
IC50: 420 nM, maximal inhibition at 10 mM | Homo sapiens | Wortmannin | |
| 0.019 | - |
IC50: 0.019 mM, maximal inhibition at 1 mM | Homo sapiens | LY294002 | |
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