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Literature summary for 2.7.1.151 extracted from

  • Resnick, A.C.; Snowman, A.M.; Kang, B.N.; Hurt, K.J.; Snyder, S.H.; Saiardi, A.
    Inositol polyphosphate multikinase is a nuclear PI3-kinase with transcriptional regulatory activity (2005), Proc. Natl. Acad. Sci. USA, 102, 12783-12788.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.151

Cloned(Commentary)

Cloned (Comment) Organism
expression of HA-tagged enzyme in human HEK293 and in COS-7 cells Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by wortmannin and LY294002 Rattus norvegicus
additional information no inhibition by wortmannin and LY294002 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 1D-myo-inositol 4,5-bisphosphate pH 7.4, 37°C Rattus norvegicus
0.03
-
 1D-myo-inositol 4,5-bisphosphate pH 7.4, 30°C Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Rattus norvegicus 5634
-
nucleus
-
 Saccharomyces cerevisiae 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Rattus norvegicus
Mg2+
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 x * 44000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 4,5-bisphosphate Rattus norvegicus
-
 ADP + 1D-myo-inositol 3,4,5-trisphosphate
-
 ?
ATP + 1D-myo-inositol 4,5-bisphosphate Saccharomyces cerevisiae
-
 ADP + 1D-myo-inositol 3,4,5-trisphosphate
-
 ?
ATP + 1D-myo-inositol 4,5-bisphosphate Saccharomyces cerevisiae BY4741
-
 ADP + 1D-myo-inositol 3,4,5-trisphosphate
-
 ?
additional information Saccharomyces cerevisiae the enzyme is involved inregulation of arginine-sensitive ArgR-Mcm1 transcriptional complex, overview ?
-
 ?
additional information Saccharomyces cerevisiae BY4741 the enzyme is involved inregulation of arginine-sensitive ArgR-Mcm1 transcriptional complex, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Saccharomyces cerevisiae
-
 wild-type strain BY4741 and several mutant strains
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by nuclear fraction preparation Rattus norvegicus
native enzyme partially by nuclear fraction preparation Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining
brain primary hippocampal and corical cultures Rattus norvegicus
-
cell culture
-
 Rattus norvegicus
-
hepatocyte primary Rattus norvegicus
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
 partially purified enzyme Rattus norvegicus
0.7
-
 partially purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 4,5-bisphosphate
-
 Rattus norvegicus ADP + 1D-myo-inositol 3,4,5-trisphosphate
-
 ?
ATP + 1D-myo-inositol 4,5-bisphosphate
-
 Saccharomyces cerevisiae ADP + 1D-myo-inositol 3,4,5-trisphosphate
-
 ?
ATP + 1D-myo-inositol 4,5-bisphosphate the enzyme is specific for 1D-myo-inositol 4,5-bisphosphate Rattus norvegicus ADP + 1D-myo-inositol 3,4,5-trisphosphate product identification ?
ATP + 1D-myo-inositol 4,5-bisphosphate the enzyme is specific for 1D-myo-inositol 4,5-bisphosphate Saccharomyces cerevisiae ADP + 1D-myo-inositol 3,4,5-trisphosphate product identification ?
ATP + 1D-myo-inositol 4,5-bisphosphate
-
 Saccharomyces cerevisiae BY4741 ADP + 1D-myo-inositol 3,4,5-trisphosphate
-
 ?
ATP + 1D-myo-inositol 4,5-bisphosphate the enzyme is specific for 1D-myo-inositol 4,5-bisphosphate Saccharomyces cerevisiae BY4741 ADP + 1D-myo-inositol 3,4,5-trisphosphate product identification ?
additional information the enzyme is involved inregulation of arginine-sensitive ArgR-Mcm1 transcriptional complex, overview Saccharomyces cerevisiae ?
-
 ?
additional information the enzyme also performs the inositol phosphate 3-kinase reaction, EC 2.7.1.127 Rattus norvegicus ?
-
 ?
additional information the enzyme performs also the inositol phosphate 3-kinase reaction, EC 2.7.1.127 Saccharomyces cerevisiae ?
-
 ?
additional information the enzyme is involved inregulation of arginine-sensitive ArgR-Mcm1 transcriptional complex, overview Saccharomyces cerevisiae BY4741 ?
-
 ?
additional information the enzyme performs also the inositol phosphate 3-kinase reaction, EC 2.7.1.127 Saccharomyces cerevisiae BY4741 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
Arg82
-
 Saccharomyces cerevisiae
ArgRIII
-
 Saccharomyces cerevisiae
inositol polyphosphate multikinase
-
 Rattus norvegicus
inositol polyphosphate multikinase
-
 Saccharomyces cerevisiae
Ipk2
-
 Saccharomyces cerevisiae
IPMK
-
 Rattus norvegicus
IPMK
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Rattus norvegicus
7.4
-
 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus
ATP
-
 Saccharomyces cerevisiae