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Literature summary for 2.7.1.151 extracted from

  • Stevenson-Paulik, J.; Odom, A.R.; York, J.D.
    Molecular and biochemical characterization of two plant inositol polyphosphate 6-/3-/5-kinases (2002), J. Biol. Chem., 277, 42711-42718.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isozyme Ipk2alpha, location on chromosome 5, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant isozyme in Escherichia coli with or without GST-fusion tag, functional complementation of an enzyme-deficient yeast strain Arabidopsis thaliana
isozyme Ipk2beta, location on chromosome 5, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant isozyme in Escherichia coli with or without GST-fusion tag, functional complementation of an enzyme-deficient yeast strain Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
D100A isozyme IPK2beta, site-directed mutagenesis, inactive mutant Arabidopsis thaliana
D98A isozyme IPK2alpha, site-directed mutagenesis, inactive mutant Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
1D-myo-inositol 1,4,5-trisphosphate pH 7.5, 37°C, recombinant isozyme Ipk2alpha Arabidopsis thaliana
0.016
-
1D-myo-inositol 1,3,4,5-tetrakisphosphate pH 7.5, 37°C, recombinant isozyme Ipk2alpha Arabidopsis thaliana
0.032
-
1D-myo-inositol 1,4,5,6-tetrakisphosphate pH 7.5, 37°C, recombinant isozyme Ipk2alpha Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
Arabidopsis thaliana
Mg2+
-
Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FLT2 isozyme Ipk2beta; isozyme Ipk2beta
-
Arabidopsis thaliana Q9LY23 isozyme Ipk2alpha; isozyme Ipk2alpha
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-fusion isozymes from Escherichia coli by glutathione affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
additional information ubiquitous expression of isoyzme Ipk2beta Arabidopsis thaliana
-
additional information ubiquitous expression of isozyme Ipk2alpha Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
silique
-
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1,2,3,4,6-pentakisphosphate
-
Arabidopsis thaliana ADP + 1D-myo-inositol 1,2,3,4,5,6-hexakisphosphate
-
?
ATP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
Arabidopsis thaliana ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
?
ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
Arabidopsis thaliana ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
?
ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
Arabidopsis thaliana ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
?
ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
Arabidopsis thaliana ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
?
additional information substrate specificity, no formation of 1D-myo-inositol 1,3,4,5,6-pentakisphosphate from 1D-myo-inositol 1,4,5-trisphosphate Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
IP3/IP4 6-/3-kinase
-
Arabidopsis thaliana
Ipk2
-
Arabidopsis thaliana
More the enzyme belongs to the IPK family of enzymes Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
1 min, inactivation, purified recombinant isozyme Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Arabidopsis thaliana