Literature summary for 2.7.1.150 extracted from

Venkatareddy, M.; Verma, R.; Kalinowski, A.; Patel, S.R.; Shisheva, A.; Garg, P.
Distinct requirements for vacuolar protein sorting 34 downstream effector phosphatidylinositol 3-phosphate 5-kinase in podocytes versus proximal tubular cells (2016), J. Am. Soc. Nephrol., 27, 2702-2719.

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Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate	Mus musculus	-	ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q9Z1T6	gene PIKfyve	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
podocyte	-	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate	-	Mus musculus	ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate	-	?

Synonyms

Synonyms	Comment	Organism
PIKfyve	-	Mus musculus
PtdIns 3-phosphate 5-kinase	-	Mus musculus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Mus musculus

General Information

General Information	Comment	Organism
malfunction	the vacuolation phenotype in cultured Vps34 (EC 2.7.1.137)-deficient podocytes is caused by the absence of a substrate for the Vps34 downstream effector PtdIns 3-phosphate 5-kinase, which phosphorylates Vps34-generated 1-phosphatidyl-1D-myo-inositol 3-phosphate to produce + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate. PtdIns 3-phosphate 5-kinase perturbation and 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate reduction result in massive membrane vacuolation along the endosomal system. Genetic deletion of the enzyme in endocytically active proximal tubular cells results in the development of large cytoplasmic vacuoles caused by arrested endocytic traffic progression at a late-endosome stage, while deletion of the enzyme in glomerular podocytes does not significantly alter the endosomal morphology, even in age 18-month-old mice	Mus musculus

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Release 2023.1 (January 2023)