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Literature summary for 2.7.1.15 extracted from
- A deoxyribokinase from Lactobacillus plantarum (1959), J. Biol. Chem., 234, 481-487.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 50 mM | Lactiplantibacillus plantarum |  |
| Mg2+ | absolute requirement, optimal ratio of Mg2+ to ATP is 1.5 : 1, higher concentrations inhibit | Lactiplantibacillus plantarum | |
| Mn2+ | 50 mM inhibit | Lactiplantibacillus plantarum | |
| additional information | not: n-ethylmaleimide | Lactiplantibacillus plantarum | |
| p-chloromercuribenzoate | - |
Lactiplantibacillus plantarum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
2-deoxy-D-ribose | 37°C, pH 7.2 | Lactiplantibacillus plantarum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolute requirement, optimal ratio of Mg2+ to ATP is 1.5 : 1, higher concentrations inhibit | Lactiplantibacillus plantarum | |
| Mn2+ | cannot replace Mg2+ in the kinase reaction, 50 mM inhibit | Lactiplantibacillus plantarum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-deoxy-D-ribose | Lactiplantibacillus plantarum | - |
ADP + 2-deoxy-D-ribose 5-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactiplantibacillus plantarum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lactiplantibacillus plantarum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 77 | - |
with D-ribose as substrate | Lactiplantibacillus plantarum |
| 89 | - |
with 2-deoxy-D-ribose as substrate | Lactiplantibacillus plantarum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C gamma gel supernatants lose 64% activity in 3 months | Lactiplantibacillus plantarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-deoxy-D-ribose | - |
Lactiplantibacillus plantarum | ADP + 2-deoxy-D-ribose 5-phosphate | - |
? | |
| ATP + D-ribose | - |
Lactiplantibacillus plantarum | ADP + D-ribose 5-phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Lactiplantibacillus plantarum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
5 min, complete inactivation | Lactiplantibacillus plantarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 7.3 | 2-deoxy-D-ribose | Lactiplantibacillus plantarum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | pH 5.5: about 45% of activity maximum, pH 9.0: about 55% of activity maximum | Lactiplantibacillus plantarum |
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Release 2023.1 (January 2023)
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