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Literature summary for 2.7.1.149 extracted from

  • Clarke, J.H.; Giudici, M.L.; Burke, J.E.; Williams, R.L.; Maloney, D.J.; Marugan, J.; Irvine, R.F.
    The function of phosphatidylinositol 5-phosphate 4-kinase gamma (PI5P4Kgamma) explored using a specific inhibitor that targets the PI5P-binding site (2015), Biochem. J., 466, 359-367.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.149

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
NIH-12848 full designation NCGC00012848-02, specific inhibitor of isoform PI5P4Kgamma, does not inhibit the alpha and beta PI5P4K isoforms at concentrations up to 0.1 mM; inhibitor of isoform PI5P4Kgamma only Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate Mus musculus
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography Mus musculus
glutathione Sepharose column chromatography, and gel filtration Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
cortical collecting duct cell
-
 Mus musculus
-
kidney cortical collecting duct cell
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate
-
 Mus musculus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

Synonyms

Synonyms Comment Organism
phosphatidylinositol 5-phosphate 4-kinase
-
 Mus musculus
phosphatidylinositol 5-phosphate 4-kinase gamma
-
 Mus musculus
PI5P4Kgamma
-
 Mus musculus
PI5P4Kgamma isoform Mus musculus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.001
-
 at pH 7.5 and 30°C Mus musculus NIH-12848
0.001
-
 at 22°C, pH not specified in the publication Mus musculus NIH-12848

Expression

Organism Comment Expression
Mus musculus the expression of isoform PI5P4Kgamma increases when the cells grow to confluence and polarize up